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Journal of Bone and Mineral Metabolism
Published in: Journal of Bone and Mineral Metabolism 5/2018

01-09-2018 | Original Article

Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone

Authors: Tomoyo Tanaka, Mitsuhiro Hoshijima, Junko Sunaga, Takashi Nishida, Mana Hashimoto, Naoya Odagaki, Ryuta Osumi, Taiji Aadachi, Hiroshi Kamioka

Published in: Journal of Bone and Mineral Metabolism | Issue 5/2018

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Abstract

Osteocytes form a three-dimensional (3D) cellular network within the mineralized bone matrix. The cellular network has important roles in mechanosensation and mechanotransduction related to bone homeostasis. We visualized the embedded osteocyte network in chick calvariae and observed the flow-induced Ca2+ signaling in osteocytes using 3D time-lapse imaging. In response to the flow, intracellular Ca2+ ([Ca2+]i) significantly increased in developmentally mature osteocytes in comparison with young osteocytes in the bone matrix. To investigate the differences in response between young and developmentally mature osteocytes in detail, we evaluated the expression of osteocyte-related genes using the osteocyte-like cell line MLO-Y4, which was 3D-cultured within type I collagen gels. We found that the c-Fos, Cx43, Panx3, Col1a1, and OCN mRNA levels significantly increased on day 15 in comparison with day 7. These findings indicate that developmentally mature osteocytes are more responsive to mechanical stress than young osteocytes and have important functions in bone formation and remodeling.
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Metadata
Title
Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone
Authors
Tomoyo Tanaka
Mitsuhiro Hoshijima
Junko Sunaga
Takashi Nishida
Mana Hashimoto
Naoya Odagaki
Ryuta Osumi
Taiji Aadachi
Hiroshi Kamioka
Publication date
01-09-2018
Publisher
Springer Japan
Published in
Journal of Bone and Mineral Metabolism / Issue 5/2018
Print ISSN: 0914-8779
Electronic ISSN: 1435-5604
DOI
https://doi.org/10.1007/s00774-017-0868-x

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Keynote webinar | Spotlight on medication adherence

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WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

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