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EJNMMI Research
Published in: EJNMMI Research 1/2016

Open Access 01-12-2016 | Original research

An exploratory study of volumetric analysis for assessing tumor response with 18F-FAZA PET/CT in patients with advanced non-small-cell lung cancer (NSCLC)

Authors: Gerald S. M. A. Kerner, Vikram R. Bollineni, Thijo J. N. Hiltermann, Nanna M. Sijtsema, Alexander Fischer, Alphons H. H. Bongaerts, Jan Pruim, Harry J. M. Groen

Published in: EJNMMI Research | Issue 1/2016

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Abstract

Background

Hypoxia is associated with resistance to chemotherapy and radiotherapy and is randomly distributed within malignancies. Characterization of changes in intratumoral hypoxic regions is possible with specially developed PET tracers such as 18F-fluoroazomycin arabinoside (18F-FAZA) while tumor metabolism can be measured with 2-deoxy-2-[18F]fluoro-d-glucose (18F-FDG). The purpose of this study was to study the effects of chemotherapy on 18F-FAZA and 18F-FDG uptake simultaneously in non-small-cell lung cancer (NSCLC) patients

Methods

At baseline and after the second chemotherapy cycle, both PET/CT with 18F-FDG and 18F-FAZA was performed in seven patients with metastasized NSCLC. 18F-FAZA and 18F-FDG scans were aligned with deformable image registration using Mirada DBx. The primary tumors were contoured, and on the 18F-FDG scan, volumes of interest (VOI) were drawn using a 41 % adaptive threshold technique. Subsequently, the resulting VOI was transferred to the 18F-FAZA scan. 18F-FAZA maximum tumor-to-background (T/Bgmax) ratio and the fractional hypoxic volume (FHV) were assessed. Measurements were corrected for partial volume effects. Finally, a voxel-by-voxel analysis of the primary tumor was performed to assess regional uptake differences.

Results

In the primary tumor of all seven patients, median 18F-FDG standard uptake value (SUVmax) decreased significantly (p = 0.03). There was no significant decrease in 18F-FAZA uptake as measured with T/Bgmax (p = 0.24) or the FHV (p = 0.35). Additionally, volumetric voxel-by-voxel analysis showed that low hypoxic tumors did not significantly change in hypoxic status between baseline and two cycles of chemotherapy, whereas highly hypoxic tumors did. Individualized volumetric voxel-by-voxel analysis revealed that hypoxia and metabolism were not associated before and after 2 cycles of chemotherapy.

Conclusions

Tumor hypoxia and metabolism are independent dynamic events as measured by 18F-FAZA PET and 18F-FDG PET, both prior to and after treatment with chemotherapy in NSCLC patients.
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Metadata
Title
An exploratory study of volumetric analysis for assessing tumor response with 18F-FAZA PET/CT in patients with advanced non-small-cell lung cancer (NSCLC)
Authors
Gerald S. M. A. Kerner
Vikram R. Bollineni
Thijo J. N. Hiltermann
Nanna M. Sijtsema
Alexander Fischer
Alphons H. H. Bongaerts
Jan Pruim
Harry J. M. Groen
Publication date
01-12-2016
Publisher
Springer Berlin Heidelberg
Published in
EJNMMI Research / Issue 1/2016
Electronic ISSN: 2191-219X
DOI
https://doi.org/10.1186/s13550-016-0187-6

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