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Immunity & Ageing
Published in: Immunity & Ageing 1/2024

Open Access 01-12-2024 | Research

An aging-related immune landscape in the hematopoietic immune system

Authors: Jianjie Lv, Chun Zhang, Xiuxing Liu, Chenyang Gu, Yidan Liu, Yuehan Gao, Zhaohao Huang, Qi Jiang, Binyao Chen, Daquan He, Tianfu Wang, Zhuping Xu, Wenru Su

Published in: Immunity & Ageing | Issue 1/2024

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Abstract

Background

Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques.

Results

We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process.

Conclusions

Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging.
Appendix
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Metadata
Title
An aging-related immune landscape in the hematopoietic immune system
Authors
Jianjie Lv
Chun Zhang
Xiuxing Liu
Chenyang Gu
Yidan Liu
Yuehan Gao
Zhaohao Huang
Qi Jiang
Binyao Chen
Daquan He
Tianfu Wang
Zhuping Xu
Wenru Su
Publication date
01-12-2024
Publisher
BioMed Central
Published in
Immunity & Ageing / Issue 1/2024
Electronic ISSN: 1742-4933
DOI
https://doi.org/10.1186/s12979-023-00403-2

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