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01-10-2019 | Alkaloids | ORIGINAL ARTICLE

Psychotria leiocarpa Extract and Vincosamide Reduce Chemically-Induced Inflammation in Mice and Inhibit the Acetylcholinesterase Activity

Authors: Anelise Samara Nazari Formagio, Carla Roberta Ferreira Volobuff, Candida Aparecida Leite Kassuya, Claudia Andréa Lima Cardoso, Maria do Carmo Vieira, Zefa Valdevina Pereira, Mariane Cristovão Bagatin, Gisele de Freitas Gauze

Published in: Inflammation | Issue 5/2019

Abstract

Species from Psychotria are used in folk medicine against inflammatory diseases, respiratory disturbances, and anti-hallucinogenic. In the present study, the compound vincosamide (PL-1) was identified for the first time in methanolic extract of the Psychotria leiocarpa (ME-PL) leaves, as well as the anti-inflammatory and anticholinesteric effects in rodents and molecular docking simulations. The fractionation of the chloroform fraction (CF-PL) through chromatographic methods afforded the known compound PL-1. The anti-inflammatory activity of the ME-PL (30, 100, and 300 mg/kg) and PL-1 (3, 30, and 100 mg/kg) was analyzed using experimental models: paw edema, pleurisy, and mechanical and thermal hyperalgesia induced by carrageenan. The anticholinesterase activity of the ME-PL (30 and 100 mg/kg) and PL-1 (30 mg/kg) was showed by acetylcholinesterase (AChE) inhibitory in brain structures. The molecular docking simulations were performed using Molegro Virtual Docker v6.0. Overall, the results indicated that ME-PL and PL-1 demonstrated an anti-edematogenic effect in Cg-induced paw edema, leukocyte migration in the pleurisy model, and significantly reduced mechanical hyperalgesia, cold response to acetone in mice. The samples exhibited maximal inhibition of enzyme acetylcholinesterase (AChE) in the frontal cortex. The molecular coupling of PL-1 with the AChE showed significant interactions with the catalytic and peripheral site, corroborating the activity presented in the inhibition assay. The acute administration of ME-PL did not cause signs of toxicity in the treated animals. The results showed that P. leiocarpa inhibited AChE and anti-inflammatory activity, and alkaloid vincosamide could be responsible, at least in part, for the observed effects, supporting the popular use of this genus.

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Title: Psychotria leiocarpa Extract and Vincosamide Reduce Chemically-Induced Inflammation in Mice and Inhibit the Acetylcholinesterase Activity
Authors: Anelise Samara Nazari Formagio
Carla Roberta Ferreira Volobuff
Candida Aparecida Leite Kassuya
Claudia Andréa Lima Cardoso
Maria do Carmo Vieira
Zefa Valdevina Pereira
Mariane Cristovão Bagatin
Gisele de Freitas Gauze
Publication date: 01-10-2019
Publisher: Springer US
Keywords: Alkaloids
Alkaloids
Edema
Published in: Inflammation / Issue 5/2019
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-019-01018-w

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