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Journal of Cardiovascular Translational Research

Published in:

01-04-2011

Adult Human Adipose Tissue Contains Several Types of Multipotent Cells

Authors: Tiziano Tallone, Claudio Realini, Andreas Böhmler, Christopher Kornfeld, Giuseppe Vassalli, Tiziano Moccetti, Silvana Bardelli, Gianni Soldati

Published in: Journal of Cardiovascular Translational Research | Issue 2/2011

Abstract

Multipotent mesenchymal stromal cells (MSCs) are a type of adult stem cells that can be easily isolated from various tissues and expanded in vitro. Many reports on their pluripotency and possible clinical applications have raised hopes and interest in MSCs. In an attempt to unify the terminology and the criteria to label a cell as MSC, in 2006 the International Society for Cellular Therapy (ISCT) proposed a standard set of rules to define the identity of these cells. However, MSCs are still extracted from different tissues, by diverse isolation protocols, are cultured and expanded in different media and conditions. All these variables may have profound effects on the selection of cell types and the composition of heterogeneous subpopulations, on the selective expansion of specific cell populations with totally different potentials and ergo, on the long-term fate of the cells upon in vitro culture. Therefore, specific molecular and cellular markers that identify MSCs subsets as well as standardization of expansion protocols for these cells are urgently needed. Here, we briefly discuss new useful markers and recent data supporting the rapidly emerging concept that many different types of progenitor cells are found in close association with blood vessels. This knowledge may promote the necessary technical improvements required to reduce variability and promote higher efficacy and safety when isolating and expanding these cells for therapeutic use. In the light of the discussed data, particularly the identification of new markers, and advances in the understanding of fundamental MSC biology, we also suggest a revision of the 2006 ISCT criteria.

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Title: Adult Human Adipose Tissue Contains Several Types of Multipotent Cells
Authors: Tiziano Tallone
Claudio Realini
Andreas Böhmler
Christopher Kornfeld
Giuseppe Vassalli
Tiziano Moccetti
Silvana Bardelli
Gianni Soldati
Publication date: 01-04-2011
Publisher: Springer US
Published in: Journal of Cardiovascular Translational Research / Issue 2/2011
Print ISSN: 1937-5387
Electronic ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-011-9257-3

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