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01-03-2019 | Original Contribution

Adipose tissue fatty acids present in dairy fat and risk of stroke: the Danish Diet, Cancer and Health cohort

Authors: Anne Sofie Dam Laursen, Christina Catherine Dahm, Søren Paaske Johnsen, Erik Berg Schmidt, Kim Overvad, Marianne Uhre Jakobsen

Published in: European Journal of Nutrition | Issue 2/2019

Abstract

Purpose

The role of dairy fat for the risk of stroke is not yet clear. Adipose tissue reflects long-term fatty acid intake and metabolism. We, therefore, investigated associations for percentages of adipose tissue fatty acids, for which dairy products are a major source (12:0, 14:0, 14:1 cis-9, 15:0, 17:0, 18:1 trans-11 and 18:2 cis-9, trans-11), with incident total stroke and stroke subtypes.

Methods

We conducted a case–cohort study within the Danish Diet, Cancer and Health cohort, including all incident stroke cases (n = 2108) and a random sample of the total cohort (n = 3186). The fatty acid composition of adipose tissue biopsies was determined by gas chromatography and specific fatty acids were expressed as percentage of total fatty acids. Stroke cases were identified in the Danish National Patient Registry and the diagnoses were individually verified.

Results

We recorded 2108 stroke cases of which 1745 were ischemic, 249 were intracerebral hemorrhages and 102 were subarachnoid hemorrhages. We observed a lower rate of ischemic stroke for a higher adipose tissue percentage of 12:0, 14:0, 15:0, 17:0, 18:1 trans-11 and 18:2 cis-9, trans-11. Adipose tissue percentages of 15:0 and 18:1 trans-11 were also inversely associated with intracerebral hemorrhage, whereas no associations between the adipose tissue fatty acids and subarachnoid hemorrhage were observed. No associations between 14:1 cis-9 and ischemic or hemorrhagic stroke were found.

Conclusions

Our results suggest that a larger percentage in adipose tissue of fatty acids for which dairy products are a major source is associated with a lower rate of ischemic stroke.

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Goldstein LB, Bushnell CD, Adams RJ, Appel LJ, Braun LT, Chaturvedi S, Creager MA, Culebras A, Eckel RH, Hart RG, Hinchey JA, Howard VJ, Jauch EC, Levine SR, Meschia JF, Moore WS, Nixon JV, Pearson TA,, Outcomes R (2011) American Heart Association Stroke C, Council on Cardiovascular N, Council on E, Prevention, Council for High Blood Pressure R, Council on Peripheral Vascular D, Interdisciplinary Council on Quality of C. Guidelines for the primary prevention of stroke: a guideline for healthcare professionals from the American Heart Association/American Stroke Association. Stroke 42(2):517–584. https://doi.org/10.1161/STR.0b013e3181fcb238 CrossRefPubMed

Appel LJ, Moore TJ, Obarzanek E, Vollmer WM, Svetkey LP, Sacks FM, Bray GA, Vogt TM, Cutler JA, Windhauser MM, Lin PH, Karanja N (1997) A clinical trial of the effects of dietary patterns on blood pressure. DASH Collaborative Research Group. N Engl J Med 336(16):1117–1124. https://doi.org/10.1056/nejm199704173361601 CrossRefPubMed

Iggman D, Gustafsson IB, Berglund L, Vessby B, Marckmann P, Riserus U (2011) Replacing dairy fat with rapeseed oil causes rapid improvement of hyperlipidaemia: a randomized controlled study. J Intern Med 270(4):356–364. https://doi.org/10.1111/j.1365-2796.2011.02383.x CrossRefPubMed

Mensink RP, Zock PL, Kester AD, Katan MB (2003) Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta-analysis of 60 controlled trials. Am J Clin Nutr 77(5):1146–1155CrossRefPubMed

Chiu S, Bergeron N, Williams PT, Bray GA, Sutherland B, Krauss RM (2016) Comparison of the DASH (Dietary Approaches to Stop Hypertension) diet and a higher-fat DASH diet on blood pressure and lipids and lipoproteins: a randomized controlled trial. Am J Clin Nutr 103(2):341–347. https://doi.org/10.3945/ajcn.115.123281 CrossRefPubMed

de Goede J, Soedamah-Muthu SS, Pan A, Gijsbers L, Geleijnse JM (2016) Dairy consumption and risk of stroke: a systematic review and updated dose-response meta-analysis of prospective cohort studies. J Am Heart Assoc. https://doi.org/10.1161/jaha.115.002787 CrossRefPubMedPubMedCentral

Arner P, Bernard S, Salehpour M, Possnert G, Liebl J, Steier P, Buchholz BA, Eriksson M, Arner E, Hauner H, Skurk T, Ryden M, Frayn KN, Spalding KL (2011) Dynamics of human adipose lipid turnover in health and metabolic disease. Nature 478(7367):110–113. https://doi.org/10.1038/nature10426 CrossRefPubMedPubMedCentral

Brevik A, Veierod MB, Drevon CA, Andersen LF (2005) Evaluation of the odd fatty acids 15:0 and 17:0 in serum and adipose tissue as markers of intake of milk and dairy fat. Eur J Clin Nutr 59(12):1417–1422. https://doi.org/10.1038/sj.ejcn.1602256 CrossRefPubMed

Wolk A, Vessby B, Ljung H, Barrefors P (1998) Evaluation of a biological marker of dairy fat intake. Am J Clin Nutr 68(2):291–295CrossRefPubMed

10.

Wolk A, Furuheim M, Vessby B (2001) Fatty acid composition of adipose tissue and serum lipids are valid biological markers of dairy fat intake in men. J Nutr 131(3):828–833CrossRefPubMed

11.

Katan MB, Deslypere JP, van Birgelen AP, Penders M, Zegwaard M (1997) Kinetics of the incorporation of dietary fatty acids into serum cholesteryl esters, erythrocyte membranes, and adipose tissue: an 18-month controlled study. J Lipid Res 38(10):2012–2022PubMed

12.

Jiang J, Wolk A, Vessby B (1999) Relation between the intake of milk fat and the occurrence of conjugated linoleic acid in human adipose tissue. Am J Clin Nutr 70(1):21–27CrossRefPubMed

13.

Bonham MP, Linderborg KM, Dordevic A, Larsen AE, Nguo K, Weir JM, Gran P, Luotonen MK, Meikle PJ, Cameron-Smith D, Kallio HP, Sinclair AJ (2013) Lipidomic profiling of chylomicron triacylglycerols in response to high fat meals. Lipids 48(1):39–50. https://doi.org/10.1007/s11745-012-3735-5 CrossRefPubMed

14.

Pallister T, Haller T, Thorand B, Altmaier E, Cassidy A, Martin T, Jennings A, Mohney RP, Gieger C, MacGregor A, Kastenmuller G, Metspalu A, Spector TD, Menni C (2016) Metabolites of milk intake: a metabolomic approach in UK twins with findings replicated in two European cohorts. Eur J Nutr. https://doi.org/10.1007/s00394-016-1278-x CrossRefPubMedPubMedCentral

15.

Woodward M, Tunstall-Pedoe H, Batty GD, Tavendale R, Hu FB, Czernichow S (2011) The prognostic value of adipose tissue fatty acids for incident cardiovascular disease: results from 3944 subjects in the Scottish Heart Health Extended Cohort Study. Eur Heart J 32(11):1416–1423. https://doi.org/10.1093/eurheartj/ehr036 CrossRefPubMedPubMedCentral

16.

Yakoob MY, Shi P, Hu FB, Campos H, Rexrode KM, Orav EJ, Willett WC, Mozaffarian D (2014) Circulating biomarkers of dairy fat and risk of incident stroke in US men and women in 2 large prospective cohorts. Am J Clin Nutr 100(6):1437–1447. https://doi.org/10.3945/ajcn.114.083097 CrossRefPubMedPubMedCentral

17.

Warensjo E, Smedman A, Stegmayr B, Hallmans G, Weinehall L, Vessby B, Johansson I (2009) Stroke and plasma markers of milk fat intake—a prospective nested case–control study. Nutr J 8:21. https://doi.org/10.1186/1475-2891-8-21 CrossRefPubMedPubMedCentral

18.

Yaemsiri S, Sen S, Tinker LF, Robinson WR, Evans RW, Rosamond W, Wasserthiel-Smoller S, He K (2013) Serum fatty acids and incidence of ischemic stroke among postmenopausal women. Stroke 44(10):2710–2717. https://doi.org/10.1161/strokeaha.111.000834 CrossRefPubMedPubMedCentral

19.

Yamagishi K, Folsom AR, Steffen LM (2013) Plasma fatty acid composition and incident ischemic stroke in middle-aged adults: the Atherosclerosis Risk in Communities (ARIC) Study. Cerebrovasc Dis 36(1):38–46. https://doi.org/10.1159/000351205 CrossRefPubMed

20.

Tjonneland A, Olsen A, Boll K, Stripp C, Christensen J, Engholm G, Overvad K (2007) Study design, exposure variables, and socioeconomic determinants of participation in Diet, Cancer and Health: a population-based prospective cohort study of 57,053 men and women in Denmark. Scand J Public Health 35(4):432–441. https://doi.org/10.1080/14034940601047986 CrossRefPubMed

21.

Beynen AC, Katan MB (1985) Rapid sampling and long-term storage of subcutaneous adipose-tissue biopsies for determination of fatty acid composition. Am J Clin Nutr 42(2):317–322CrossRefPubMed

22.

Tjonneland A, Overvad K, Thorling E, Ewertz M (1993) Adipose tissue fatty acids as biomarkers of dietary exposure in Danish men and women. Am J Clin Nutr 57(5):629–633CrossRefPubMed

23.

Rix TA, Joensen AM, Riahi S, Lundbye-Christensen S, Overvad K, Schmidt EB (2013) Marine n-3 fatty acids in adipose tissue and development of atrial fibrillation: a Danish cohort study. Heart 99(20):1519–1524. https://doi.org/10.1136/heartjnl-2013-304385 CrossRefPubMed

24.

Schmidt M, Schmidt SA, Sandegaard JL, Ehrenstein V, Pedersen L, Sorensen HT (2015) The Danish National Patient Registry: a review of content, data quality, and research potential. Clin Epidemiol 7:449–490. https://doi.org/10.2147/clep.s91125 CrossRefPubMedPubMedCentral

25.

Luhdorf P, Overvad K, Schmidt EB, Johnsen SP, Bach FW (2017) Predictive value of stroke discharge diagnoses in the Danish National Patient Register. Scand J Public Health 45(6):630–636. https://doi.org/10.1177/1403494817716582 CrossRefPubMed

26.

Adams HP, Bendixen BH, Kappelle LJ, Biller J, Love BB, Gordon DL, Marsh EE (1993) Classification of subtype of acute ischemic stroke. Definitions for use in a multicenter clinical trial. TOAST. Trial of Org 10172 in Acute Stroke Treatment. Stroke 24(1):35–41. https://doi.org/10.1161/01.str.24.1.35 CrossRefPubMed

27.

Kalbfleisch JD, Lawless JF (1988) Likelihood analysis of multi-state models for disease incidence and mortality. Stat Med 7(1–2):149–160CrossRefPubMed

28.

Petersen L, Sorensen TI, Andersen PK (2003) Comparison of case-cohort estimators based on data on premature death of adult adoptees. Stat Med 22(24):3795–3803. https://doi.org/10.1002/sim.1672 CrossRefPubMed

29.

Iso H, Jacobs DR Jr, Wentworth D, Neaton JD, Cohen JD (1989) Serum cholesterol levels and six-year mortality from stroke in 350,977 men screened for the multiple risk factor intervention trial. N Engl J Med 320(14):904–910. https://doi.org/10.1056/nejm198904063201405 CrossRefPubMed

30.

Leppala JM, Virtamo J, Fogelholm R, Albanes D, Heinonen OP (1999) Different risk factors for different stroke subtypes: association of blood pressure, cholesterol, and antioxidants. Stroke 30(12):2535–2540CrossRefPubMed

31.

Collins R, Reith C, Emberson J, Armitage J, Baigent C, Blackwell L, Blumenthal R, Danesh J, Smith GD, DeMets D, Evans S, Law M, MacMahon S, Martin S, Neal B, Poulter N, Preiss D, Ridker P, Roberts I, Rodgers A, Sandercock P, Schulz K, Sever P, Simes J, Smeeth L, Wald N, Yusuf S, Peto R Interpretation of the evidence for the efficacy and safety of statin therapy. Lancet 388 (10059):2532–2561. https://doi.org/10.1016/S0140-6736(16)31357-5

32.

Gaucheron F (2011) Milk and dairy products: a unique micronutrient combination. J Am Coll Nutr 30(5 Suppl 1):400 s–409 sCrossRef

33.

Larsson SC, Orsini N, Wolk A (2012) Dietary magnesium intake and risk of stroke: a meta-analysis of prospective studies. Am J Clin Nutr 95(2):362–366. https://doi.org/10.3945/ajcn.111.022376 CrossRefPubMed

34.

Larsson SC, Orsini N, Wolk A (2013) Dietary calcium intake and risk of stroke: a dose–response meta-analysis. Am J Clin Nutr 97(5):951–957. https://doi.org/10.3945/ajcn.112.052449 CrossRefPubMed

35.

Woods VB, Fearon AM (2009) Dietary sources of unsaturated fatty acids for animals and their transfer into meat, milk and eggs: a review. Livest Sci 126(1–3):1–20. https://doi.org/10.1016/j.livsci.2009.07.002 CrossRef

Title: Adipose tissue fatty acids present in dairy fat and risk of stroke: the Danish Diet, Cancer and Health cohort
Authors: Anne Sofie Dam Laursen
Christina Catherine Dahm
Søren Paaske Johnsen
Erik Berg Schmidt
Kim Overvad
Marianne Uhre Jakobsen
Publication date: 01-03-2019
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 2/2019
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-018-1608-2

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