Top

Archives of Gynecology and Obstetrics

Published in:

01-06-2011 | Materno-fetal Medicine

Adiponectin SNP45TG is associated with gestational diabetes mellitus

Authors: Chen Fei Low, Eusni Rahayu Mohd Tohit, Pei Pei Chong, Faridah Idris

Published in: Archives of Gynecology and Obstetrics | Issue 6/2011

Abstract

Introduction

Diabetes and pregnancy can be associated in two ways: pregnancy that occurs in women who are already diabetic (diabetes of pre-gestational origin); and diabetes that occur in women who are already pregnant [gestational diabetes mellitus (GDM) (O’sullivan 1961)]. Patients with previous GDM history have higher risk of developing diabetes outside of pregnancy. Accumulating literature had suggested that adiponectin plays a role in the pathophysiology of this metabolic syndrome, and several of the common single nucleotide polymorphisms (SNP) in adiponectin gene have been identified in type 2 diabetes. Thus, one of the commonly found SNP was studied to determine its association with GDM.

Objective

To identify the association of SNP45TG with GDM.

Methods

This is a cross-sectional study involving pregnant mothers of <18 gestational weeks, who were recruited from three local antenatal clinics in Selangor, Malaysia. Their genomic DNA was extracted from EDTA treated whole blood using commercialized kit. Adiponectin gene was amplified through conventional PCR and SNP was detected using restriction enzyme SmaI. Plasma adiponectin level, fructosamine level and HbA_1c percentage were also examined.

Results

Among the 79 antenatal patients recruited, 53 patients were normal and 26 were diagnosed with GDM. Among the 53 normal patients, 18 carry TG/GG genotype. Meanwhile, among the 26 patients that were diagnosed with GDM 15 carry TG/GG genotype. Significant association was found between SNP45TG with GDM ( χ² = 4.038; P < 0.05). In addition, normal patients with TT genotype have significantly higher plasma adiponectin level compared to other groups.

Conclusion

We concluded that SNP45TG in adiponectin gene is associated with the occurrence of GDM.

Ferrara A, Kahn HS, Quesenberry CP, Riley C, Hedderson MM (2004) An increase in the incidence of gestational diabetes mellitus: Northern California, 1991–2000. Obstet Gynecol 103:526–533PubMedCrossRef

Dabelea D, Snell-Bergeon JK, Hartsfield CL, Bischoff KJ, Hamman RF, McDuffie RS (2005) Increasing prevalence of gestational diabetes mellitus (GDM) over time and by birth cohort: Kaiser Permanente of Colorado GDM Screening Program. Diabetes Care 28:579–584PubMedCrossRef

Ryo M, Nakamura T, Kihara S, Kumada M, Shibazaki S, Takahashi M, Nagai M, Matsuzawa Y, Funahashi T (2004) Adiponectin as a biomarker of the metabolic syndrome. Circ J 68:975–981PubMedCrossRef

Hotta K, Funahashi T, Bodkin NL et al (2001) Circulating concentrations of the adipocyte protein adiponectin are decreased in parallel with reduced insulin sensitivity during the progression to type 2 diabetes in rhesus monkeys. Diabetes 50(5):1126–1133PubMedCrossRef

Berg AH, Combs TP, Du X, Brownlee M, Scherer PE (2001) The adipocyte-secreted protein Acrp30 enhances hepatic insulin action. Nat Med 7:947–953PubMedCrossRef

Hotta K, Funahashi T, Arita Y, Takahashi M, Matsuda M, Okamoto Y et al (2000) Plasma concentrations of a novel adipose-specific protein, adiponectin, in type 2 diabetic patients. Arterioscler Thromb Vasc Biol 20:1595–1599PubMed

Lindsay RS, Funahashi T, Hanson RL, Matsuzawa Y, Tanaka S, Tataranni PA et al (2002) Adiponectin and development of type 2 diabetes in the Pima Indian population. Lancet 360:57–58PubMedCrossRef

Hara K, Boutin P, Mori Y, Tobe K, Dina C, Yasuda K et al (2002) Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population. Diabetes 51:536–540PubMedCrossRef

Menzaghi C, Ercolino T, Di Paola R, Berg AH, Warram JH, Scherer PE et al (2002) A haplotype at the adiponectin locus is associated with obesity and other features of the insulin resistance syndrome. Diabetes 51:2306–2312PubMedCrossRef

10.

Li LL, Kang XL, Ran XJ, Wang Y, Wang CH, Huang L, Ren J, Luo X, Mao XM (2007) Association between 45T/G polymorphism of the adiponectin gene and plasma adiponectin levels with type 2 diabetes. Clin Exp Pharmacol Physiol 34:1287–1290PubMedCrossRef

11.

Stumvoll M, Tschritter O, Fritsche A, Staiger H, Renn W, Weisser M, Machicao F, Haring H (2002) Association of the T-G Polymorphism in adiponectin (Exon 2) with obesity and insulin sensitivity interaction with family history of type 2 diabetes. Diabetes 51:37–41PubMedCrossRef

12.

Farrell PM, Engle MJ, Frantz ID et al (1982) Complications of pregnancy and fetal development. Diabetes 31:89–94PubMed

13.

Kousseff BG, Ohashi K, Ouchi N, Kihara S, Funahashi T et al (1999) Gestational diabetes mellitus (class A): a human teratogen? Am J Med Genet 83:402–408PubMedCrossRef

14.

Williams MA, Qiu CF, Muy-Rivera M, Vadachkoria S, Song T, Luthy DA (2004) Plasma adiponectin concentrations in early pregnancy and subsequent risk of gestational diabetes mellitus. J Clin Endocrinol Metab 89(5):2306–2311PubMedCrossRef

15.

Spranger J, Kroke A, Mohlig M, Bergmann MM, Ristow M, Boeing H, Pfeiffer AFH (2003) Adiponectin and protection against type 2 diabetes mellitus. Lancet 361:226–228PubMedCrossRef

16.

Lain Kristine Y, Daftary Ashi R, Ness Roberta B, Roberts James M (2008) First trimester adipocytokines concentrations and risk of developing gestational diabetes later in pregnancy. Clin Endocrinol 69(3):407–411CrossRef

17.

Retnakaran R, Hanley AJG, Raif N, Hirning CR, Connelly PW, Sermer M, Kahn SE, Zinman B (2005) Adiponectin and beta cell dysfunction in gestational diabetes: pathophysiological implications. Diabetologia 48:993–1001PubMedCrossRef

18.

Tsai PJ, Yu CH, Hsu SP, Lee YH, Huang IT, Ho SC, Chu CH (2005) Maternal plasma adiponectin concentrations at 24 to 31 weeks of gestation: negative association with gestational diabetes mellitus. Nutrition 21:1095–1099PubMedCrossRef

19.

Retnakaran R, Hanley AJG, Raif N, Connelly PW, Sermer M, Zinman B (2004) Hypoadiponectinaemia in south Asian women during pregnancy: evidence of ethnic variation in adiponectin concentration. Diabet Med 21:388–392PubMedCrossRef

20.

Retnakaran R, Hanley AJG, Raif N, Connelly PW, Sermer M, Zinman B (2004) Reduced adiponectin concentration in women with gestational diabetes. a potential factor in progression to type 2 diabetes. Diabetes Care 27:799–800PubMedCrossRef

21.

Ranheim T, Haugen F, Staff AC, Braekke K, Harsem NK, Drevon CA (2004) Adiponectin is reduced in gestational diabetes mellitus in normal weight women. Acta Obstet Gynecol Scand 83:341–347PubMed

22.

Yamauchi T, Waki H, Kamon J et al (2001) Inhibition of RXR and PPAR gamma ameliorates diet-induced obesity and type 2 diabetes. J Clin Invest 108(7):1001–1013PubMed

23.

Yamauchi T, Kamon J, Waki H, Terauchi Y et al (2001) Replenishment of the fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med 7:941–946PubMedCrossRef

24.

Kissebah AH, Sonnenberg GE, Myklebust J, Goldstein M, Broman K et al (2000) Quantitative trait loci on chromosomes 3 and 17 influence phenotypes of the metabolic syndrome. Proc Natl Acad Sci USA 97:14478–14483PubMedCrossRef

25.

Gable DR, Hurel SJ, Humphries SE (2006) Adiponectin and its gene variants as risk factors for insulin resistance, the metabolic syndrome and cardiovascular disease. Atherosclerosis 188:231–244PubMedCrossRef

26.

Gable DR, Matin J, Whittall R, Cakmak H, Li KW, Cooper J, Miller GJ, Humphries SE (2007) Common adiponectin gene variants show different effect on risk of cardiovascular disease and type 2 diabetes in European subjects. Ann Hum Genet 71:453–466PubMedCrossRef

27.

Gonzalez-Sanchez JL, Zabena CA, Martinez-Larrad MT, Fernandez-Perez C et al (2005) An SNP in the adiponectin gene is associated with decreased serum adiponectin levels and risk for impaired glucose tolerance. Obes Res 13:807–812PubMedCrossRef

28.

Zacharova J, Chiasson JL, Laakso M (2005) The common polymorphism (single nucleotide polymorphism [SNP] +45 and SNP +276) of the adiponectin gene predict the conversion from impaired glucose tolerance to type 2 diabetes. Diabetes 54:893–899PubMedCrossRef

29.

Filippi E, Sentinelli F, Trischitta V, Romeo S et al (2004) Association of the human adiponectin gene and insulin resistance. Eur J Hum Genet 12:199–205PubMedCrossRef

30.

Hara K, Boutin P, Mori Y, Tobe K et al (2002) Genetic variation in the gene encoding adiponectin is associated with an increased risk of type 2 diabetes in the Japanese population. Diabetes 51:536–540PubMedCrossRef

31.

Vasseur F, Helbecque N, Dina C et al (2002) Single nucleotide polymorphism haplotype in the both proximal promoter and exon 3 of the APM1 gene modulate adipocyte-secreted adiponectin hormone levels and contribute to the genetic risk for type 2 diabetes in French Caucasians. Hum Mol Genet 11:2607–2614PubMedCrossRef

Title: Adiponectin SNP45TG is associated with gestational diabetes mellitus
Authors: Chen Fei Low
Eusni Rahayu Mohd Tohit
Pei Pei Chong
Faridah Idris
Publication date: 01-06-2011
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 6/2011
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-010-1548-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Adiponectin SNP45TG is associated with gestational diabetes mellitus

Abstract

Introduction

Objective

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Objective

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 6/2011

Visual estimation versus gravimetric measurement of postpartum blood loss: a prospective cohort study

Successful application of vacuum-assisted closure therapy for treatment of mastitis-associated chronic breast wounds

Comparison of isosorbide mononitrate and misoprostol for cervical ripening in termination of pregnancy between 8 and 12 weeks: a randomized controlled trial

Screening for Chlamydia trachomatis in pregnancy: a retrospective analysis in a German urban area

Bladder-opening technique for hysterectomy for placenta previa percreta

Influence of the mode of delivery on maternal and neonatal outcomes: a comparison between elective cesarean section and planned vaginal delivery in a low-risk obstetric population