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Published in: Journal of Cancer Research and Clinical Oncology 3/2024

Open Access 01-03-2024 | Acute Myeloid Leukemia | Research

Pan-cancer analysis of DDIT4 identifying its prognostic value and function in acute myeloid leukemia

Authors: Fangmei Li, Jiyu Miao, Rui Liu, Ru Zhang, Aili He

Published in: Journal of Cancer Research and Clinical Oncology | Issue 3/2024

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Abstract

Background

Acute myeloid leukemia (AML) is a hematological malignancy derived from the accumulation of abnormal proliferation of infantile leukocytes in the hematopoietic system. DNA-damage-inducible transcript 4 (DDIT4) acting as a negative regulator of rapamycin inhibitor is involved in various cellular functions. Many studies have suggested that DDIT4 plays a key role in tumorigenesis. However, the role of DDIT4 in AML has been poorly studied.

Method

In this study, we analyzed the expression of DDIT4 in AML patients using The Cancer Genome Atlas and real-time polymerase chain reaction. The Chi-square test was used to assess the correlation between DDIT4 and clinical characters in AML patients. Loss-of-function experiments were implemented to investigate the role of DDIT4 in AML carcinogenesis. The R package was applied to evaluate the correlation between DDIT4 expression and immune cells.

Results

Results showed that the expression of DDIT4 was associated with Age, Cytogenetic risk, Cytogenetics and OS event. Moreover, high expression of DDIT4 led to a terrible prognosis. KEGG analysis showed that differently expressed genes (DEGs) were involved in the PI3-Akt signaling pathway. GSEA enrichment analysis displayed DEGs were correlated with apoptosis. Functional experiments presented that knocking down DDIT4 suppressed cell cycle transition/proliferation and facilitated apoptosis. In addition, DDIT4 is associated with immune infiltration.

Conclusion

Our research verified that DDIT4 can be used as a prognostic marker and a potential therapeutic target for AML.
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Metadata
Title
Pan-cancer analysis of DDIT4 identifying its prognostic value and function in acute myeloid leukemia
Authors
Fangmei Li
Jiyu Miao
Rui Liu
Ru Zhang
Aili He
Publication date
01-03-2024
Publisher
Springer Berlin Heidelberg
Published in
Journal of Cancer Research and Clinical Oncology / Issue 3/2024
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI
https://doi.org/10.1007/s00432-024-05676-8

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