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American Journal of Clinical Dermatology
Published in: American Journal of Clinical Dermatology 1/2020

01-02-2020 | Acne | Short Communication

Characterization and Analysis of the Skin Microbiota in Rosacea: A Case–Control Study

Authors: Barbara M. Rainer, Katherine G. Thompson, Corina Antonescu, Liliana Florea, Emmanuel F. Mongodin, Jonathan Bui, Alexander H. Fischer, Helena B. Pasieka, Luis A. Garza, Sewon Kang, Anna L. Chien

Published in: American Journal of Clinical Dermatology | Issue 1/2020

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Abstract

Background

The efficacy of antibiotics in rosacea treatment suggests a role for microorganisms in its pathophysiology. Growing concern over the adverse effects of antibiotic use presents a need for targeted antimicrobial treatment in rosacea.

Objective

We performed a case–control study to investigate the skin microbiota in patients with rosacea compared to controls matched by age, sex, and race.

Methods

Nineteen participants with rosacea, erythematotelangiectatic, papulopustular, or both, were matched to 19 rosacea-free controls. DNA was extracted from skin swabs of the nose and bilateral cheeks of participants. Sequencing of the V3V4 region of the bacterial 16S ribosomal RNA gene was performed using Illumina MiSeq and analyzed using QIIME/MetaStats 2.0 software.

Results

Compared with controls, skin microbiota in erythematotelangiectatic rosacea was depleted in Roseomonas mucosa (p = 0.004). Papulopustular rosacea was enriched in Campylobacter ureolyticus (p = 0.001), Corynebacterium kroppenstedtii (p = 0.008), and the oral flora Prevotella intermedia (p = 0.001). The highest relative abundance of C. kroppenstedtii was observed in patients with both erythematotelangiectatic and papulopustular rosacea (19.2%), followed by papulopustular (5.06%) and erythematotelangiectatic (1.21%) rosacea. C. kroppenstedtii was also associated with more extensive disease, with the highest relative abundance in rosacea affecting both the cheeks and nose (2.82%), followed by rosacea sparing the nose (1.93%), and controls (0.19%).

Conclusions

The skin microbiota in individuals with rosacea displays changes from that of healthy skin, suggesting that further studies examining a potential role for the skin microbiota in the pathophysiology of rosacea may be warranted.
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Metadata
Title
Characterization and Analysis of the Skin Microbiota in Rosacea: A Case–Control Study
Authors
Barbara M. Rainer
Katherine G. Thompson
Corina Antonescu
Liliana Florea
Emmanuel F. Mongodin
Jonathan Bui
Alexander H. Fischer
Helena B. Pasieka
Luis A. Garza
Sewon Kang
Anna L. Chien
Publication date
01-02-2020
Publisher
Springer International Publishing
Published in
American Journal of Clinical Dermatology / Issue 1/2020
Print ISSN: 1175-0561
Electronic ISSN: 1179-1888
DOI
https://doi.org/10.1007/s40257-019-00471-5

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