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Clinical Oral Investigations
Published in: Clinical Oral Investigations 9/2016

Open Access 01-12-2016 | Original Article

Accuracy of commercial kits and published primer pairs for the detection of periodontopathogens

Authors: Elisabeth Santigli, Eva Leitner, Gernot Wimmer, Harald H. Kessler, Gebhard Feierl, Martin Grube, Katharina Eberhard, Barbara Klug

Published in: Clinical Oral Investigations | Issue 9/2016

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Abstract

Objectives

Despite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. The aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens.

Materials and methods

Fourteen bacterial mock communities, consisting of 16 randomly assembled bacterial strains, were used as reference standard for testing kits and primers. Extracted DNA from mock communities was analyzed by PCR in-house with specific primers and forwarded for analysis to the manufacturer’s laboratory of each of the following kits: ParoCheck®Kit 20, micro-IDent®plus11, and Carpegen® Perio Diagnostik.

Results

The kits accurately detected Fusobacterium nucleatum, Prevotella intermedia/Prevotella nigrescens, Parvimonas micra, Aggregatibacter actinomycetemcomitans, Campylobacter rectus/showae, Streptococcus mitis, Streptococcus mutans, and Veillonella parvula. The in-house primers for F.nucleatum were highly specific to subtypes of the respective periopathogen. Other primers repeatedly detected oral pathogens not present in the mock communities, indicating reduced specificity.

Conclusions

The commercial kits used in this study are reliable tools to support periodontal diagnostics. Whereas the detection profile of the kits is fixed at a general specificity level, the design of primers can be adjusted to differentiate between highly specific strains. In-house primers are more error-prone. Bacterial mock communities can be established as a reference standard for any similar testing.

Clinical relevance

The tested kits render good results with selected bacterial species. Primers appear to be less useful for routine clinical diagnostics and of limited applicability in research. Basic information about the periodontopathogens identified in this study supports clinical decision-making.
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Metadata
Title
Accuracy of commercial kits and published primer pairs for the detection of periodontopathogens
Authors
Elisabeth Santigli
Eva Leitner
Gernot Wimmer
Harald H. Kessler
Gebhard Feierl
Martin Grube
Katharina Eberhard
Barbara Klug
Publication date
01-12-2016
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 9/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-016-1748-9

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