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Archives of Virology
Published in: Archives of Virology 6/2013

01-06-2013 | Original Article

A serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NP

Authors: Nattamon Siboonnan, Wittawat Wiriyarat, Chompunuch Boonarkart, Warunya Chakritbudsabong, Anan Jongkaewwattana, Pilaipan Puthavathana, Prasert Auewarakul, Ornpreya Suptawiwat

Published in: Archives of Virology | Issue 6/2013

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Abstract

We have generated a temperature-sensitive (ts) mutant from a human isolate of the H5N1 avian influenza virus by classical adaptation in cell culture. After 20 passages at low temperature, the virus showed a ts phenotype. The ts mutant also showed an attenuated phenotype after nasal inoculation in mice. Using reverse genetics, we generated reassortants carrying individual genomic segments of the wild-type and mutant viruses in an A/Puerto Rico/8/34 background, and found that the nucleoprotein (NP) gene could confer the ts phenotype. This mutant NP contains a serine-to-asparagine mutation at position 314 (S314N). The mutant NP protein showed a defect in nuclear localization at high temperature in mammalian cells.
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Metadata
Title
A serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NP
Authors
Nattamon Siboonnan
Wittawat Wiriyarat
Chompunuch Boonarkart
Warunya Chakritbudsabong
Anan Jongkaewwattana
Pilaipan Puthavathana
Prasert Auewarakul
Ornpreya Suptawiwat
Publication date
01-06-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1595-1

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