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Published in: Irish Journal of Medical Science (1971 -) 2/2011

01-06-2011 | Original Article

A novel sequence-based coa genotyping method to discriminate nosocomial methicillin-resistant Staphylococcus aureus isolates

Authors: Q. T. Li, Y. Z. Zhu, K. Dong, C. Liu, Y. H. Zhou, Y. X. Ni, X. K. Guo

Published in: Irish Journal of Medical Science (1971 -) | Issue 2/2011

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Abstract

Aim

To evaluate a novel sequence-based coa typing method in classification for nosocomial methicillin-resistant Staphylococcus aureus.

Method

D1 and D2 regions of coa gene were used to discriminate methicillin-resistant Staphylococcus aureus and compared with other classical sequence-based methods with discriminate power.

Results

All MRSA isolates were divided into 11 types and about 20 subtypes with coa typing method here. The discriminate power of this coa typing method was better than MLST or spa method.

Conclusion

It was demonstrated that this coa method is a simple, rapid, and effective technique applicable for outbreak or local nosocomial MRSA investigations in the future.
Literature
1.
go back to reference Casey AL, Lambert PA, Elliott TS (2007) Staphylococci. Int J Antimicrob Agents 29(Suppl 3):S23–S32PubMedCrossRef Casey AL, Lambert PA, Elliott TS (2007) Staphylococci. Int J Antimicrob Agents 29(Suppl 3):S23–S32PubMedCrossRef
2.
go back to reference Aires de Sousa M, de Lencastre H (2004) Bridges from hospitals to the laboratory: genetic portraits of methicillin-resistant Staphylococcus aureus clones. FEMS Immunol Med Microbiol 40:101–111PubMedCrossRef Aires de Sousa M, de Lencastre H (2004) Bridges from hospitals to the laboratory: genetic portraits of methicillin-resistant Staphylococcus aureus clones. FEMS Immunol Med Microbiol 40:101–111PubMedCrossRef
3.
go back to reference Huang TD, Almpanis C, Denis O et al (2007) Reversion of resistance in relapsing infection caused by a glycopeptide-intermediate methicillin-resistant Staphylococcus aureus isolate. Eur J Clin Microbiol Infect Dis 26:419–422PubMedCrossRef Huang TD, Almpanis C, Denis O et al (2007) Reversion of resistance in relapsing infection caused by a glycopeptide-intermediate methicillin-resistant Staphylococcus aureus isolate. Eur J Clin Microbiol Infect Dis 26:419–422PubMedCrossRef
4.
go back to reference Zhang R, Eggleston K, Rotimi V, Zeckhauser RJ (2006) Antibiotic resistance as a global threat: evidence from China, Kuwait and the United States. Glob Health 2:6CrossRef Zhang R, Eggleston K, Rotimi V, Zeckhauser RJ (2006) Antibiotic resistance as a global threat: evidence from China, Kuwait and the United States. Glob Health 2:6CrossRef
5.
go back to reference Deurenberg RH, Vink C, Kalenic S et al (2007) The molecular evolution of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 13:222–235PubMedCrossRef Deurenberg RH, Vink C, Kalenic S et al (2007) The molecular evolution of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 13:222–235PubMedCrossRef
6.
go back to reference Alonso S, Pethe K, Russell DG, Purdy GE (2007) Lysosomal killing of Mycobacterium mediated by ubiquitin-derived peptides is enhanced by autophagy. Proc Natl Acad Sci USA 104:6031–6036PubMedCrossRef Alonso S, Pethe K, Russell DG, Purdy GE (2007) Lysosomal killing of Mycobacterium mediated by ubiquitin-derived peptides is enhanced by autophagy. Proc Natl Acad Sci USA 104:6031–6036PubMedCrossRef
7.
go back to reference Zhang K, McClure JA, Elsayed S, Louie T, Conly JM (2005) Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 43:5026–5033PubMedCrossRef Zhang K, McClure JA, Elsayed S, Louie T, Conly JM (2005) Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol 43:5026–5033PubMedCrossRef
8.
go back to reference Oliveira DC, de Lencastre H (2002) Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 46:2155–2161PubMedCrossRef Oliveira DC, de Lencastre H (2002) Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 46:2155–2161PubMedCrossRef
9.
go back to reference Oliveira DC, Crisostomo I, Santos-Sanches I et al (2001) Comparison of DNA sequencing of the protein A gene polymorphic region with other molecular typing techniques for typing two epidemiologically diverse collections of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 39:574–580PubMedCrossRef Oliveira DC, Crisostomo I, Santos-Sanches I et al (2001) Comparison of DNA sequencing of the protein A gene polymorphic region with other molecular typing techniques for typing two epidemiologically diverse collections of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 39:574–580PubMedCrossRef
10.
go back to reference Harmsen D, Claus H, Witte W et al (2003) Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 41:5442–5448PubMedCrossRef Harmsen D, Claus H, Witte W et al (2003) Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 41:5442–5448PubMedCrossRef
11.
go back to reference Ip M, Yung RW, Ng TK et al (2005) Contemporary methicillin-resistant Staphylococcus aureus clones in Hong Kong. J Clin Microbiol 43:5069–5073PubMedCrossRef Ip M, Yung RW, Ng TK et al (2005) Contemporary methicillin-resistant Staphylococcus aureus clones in Hong Kong. J Clin Microbiol 43:5069–5073PubMedCrossRef
12.
go back to reference Aanensen DM, Spratt BG (2005) The multilocus sequence typing network: mlst.net. Nucleic Acids Res 33:W728–W733PubMedCrossRef Aanensen DM, Spratt BG (2005) The multilocus sequence typing network: mlst.net. Nucleic Acids Res 33:W728–W733PubMedCrossRef
13.
go back to reference Watanabe S, Ito T, Takeuchi F et al (2005) Structural comparison of ten serotypes of staphylocoagulases in Staphylococcus aureus. J Bacteriol 187:3698–3707PubMedCrossRef Watanabe S, Ito T, Takeuchi F et al (2005) Structural comparison of ten serotypes of staphylocoagulases in Staphylococcus aureus. J Bacteriol 187:3698–3707PubMedCrossRef
14.
go back to reference Silva ER, Boechat JU, Silva N (2006) Coagulase gene polymorphism of Staphylococcus aureus isolated from goat mastitis in Brazilian dairy herds. Lett Appl Microbiol 42:30–34PubMedCrossRef Silva ER, Boechat JU, Silva N (2006) Coagulase gene polymorphism of Staphylococcus aureus isolated from goat mastitis in Brazilian dairy herds. Lett Appl Microbiol 42:30–34PubMedCrossRef
15.
go back to reference Drago L, De Vecchi E, Nicola L, Gismondo MR (2007) In vitro evaluation of antibiotics’ combinations for empirical therapy of suspected methicillin resistant Staphylococcus aureus severe respiratory infections. BMC Infect Dis 7:111PubMedCrossRef Drago L, De Vecchi E, Nicola L, Gismondo MR (2007) In vitro evaluation of antibiotics’ combinations for empirical therapy of suspected methicillin resistant Staphylococcus aureus severe respiratory infections. BMC Infect Dis 7:111PubMedCrossRef
16.
go back to reference Robinson DA, Enright MC (2004) Multilocus sequence typing and the evolution of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 10:92–97PubMedCrossRef Robinson DA, Enright MC (2004) Multilocus sequence typing and the evolution of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 10:92–97PubMedCrossRef
17.
go back to reference Cramton SE, Schnell NF, Gotz F, Bruckner R (2000) Identification of a new repetitive element in Staphylococcus aureus. Infect Immun 68:2344–2348PubMedCrossRef Cramton SE, Schnell NF, Gotz F, Bruckner R (2000) Identification of a new repetitive element in Staphylococcus aureus. Infect Immun 68:2344–2348PubMedCrossRef
18.
go back to reference Yamasaki O, Kaneko J, Morizane S et al (2005) The association between Staphylococcus aureus strains carrying Panton–Valentine leukocidin genes and the development of deep-seated follicular infection. Clin Infect Dis 40:381–385PubMedCrossRef Yamasaki O, Kaneko J, Morizane S et al (2005) The association between Staphylococcus aureus strains carrying Panton–Valentine leukocidin genes and the development of deep-seated follicular infection. Clin Infect Dis 40:381–385PubMedCrossRef
19.
go back to reference Ho PL, Wang TK, Ching P et al (2007) Epidemiology and genetic diversity of methicillin-resistant Staphylococcus aureus strains in residential care homes for elderly persons in Hong Kong. Infect Control Hosp Epidemiol 28:671–678PubMedCrossRef Ho PL, Wang TK, Ching P et al (2007) Epidemiology and genetic diversity of methicillin-resistant Staphylococcus aureus strains in residential care homes for elderly persons in Hong Kong. Infect Control Hosp Epidemiol 28:671–678PubMedCrossRef
20.
go back to reference Cookson BD, Robinson DA, Monk AB et al (2007) Evaluation of molecular typing methods in characterizing a European collection of epidemic methicillin-resistant Staphylococcus aureus strains: the HARMONY collection. J Clin Microbiol 45:1830–1837PubMedCrossRef Cookson BD, Robinson DA, Monk AB et al (2007) Evaluation of molecular typing methods in characterizing a European collection of epidemic methicillin-resistant Staphylococcus aureus strains: the HARMONY collection. J Clin Microbiol 45:1830–1837PubMedCrossRef
21.
go back to reference Xu Z, Shi L, Zhang C et al (2007) Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in South China. Clin Microbiol Infect 13:980–984PubMedCrossRef Xu Z, Shi L, Zhang C et al (2007) Nosocomial infection caused by class 1 integron-carrying Staphylococcus aureus in a hospital in South China. Clin Microbiol Infect 13:980–984PubMedCrossRef
22.
go back to reference Rodrigues da Silva E, da Silva N (2005) Coagulase gene typing of Staphylococcus aureus isolated from cows with mastitis in southeastern Brazil. Can J Vet Res 69:260–264PubMed Rodrigues da Silva E, da Silva N (2005) Coagulase gene typing of Staphylococcus aureus isolated from cows with mastitis in southeastern Brazil. Can J Vet Res 69:260–264PubMed
23.
go back to reference Koreen L, Ramaswamy SV, Graviss EA et al (2004) Spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation. J Clin Microbiol 42:792–799PubMedCrossRef Koreen L, Ramaswamy SV, Graviss EA et al (2004) Spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation. J Clin Microbiol 42:792–799PubMedCrossRef
Metadata
Title
A novel sequence-based coa genotyping method to discriminate nosocomial methicillin-resistant Staphylococcus aureus isolates
Authors
Q. T. Li
Y. Z. Zhu
K. Dong
C. Liu
Y. H. Zhou
Y. X. Ni
X. K. Guo
Publication date
01-06-2011
Publisher
Springer-Verlag
Published in
Irish Journal of Medical Science (1971 -) / Issue 2/2011
Print ISSN: 0021-1265
Electronic ISSN: 1863-4362
DOI
https://doi.org/10.1007/s11845-011-0680-x

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