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BMC Infectious Diseases
Published in: BMC Infectious Diseases 1/2011

Open Access 01-12-2011 | Research article

A molecular epidemiological study of respiratory viruses detected in Japanese children with acute wheezing illness

Authors: Asako Fujitsuka, Hiroyuki Tsukagoshi, Mika Arakawa, Kazuko Goto-Sugai, Akihide Ryo, Yoshimichi Okayama, Katsumi Mizuta, Atsuyoshi Nishina, Masakazu Yoshizumi, Yoichi Kaburagi, Masahiro Noda, Masato Tashiro, Nobuhiko Okabe, Masaaki Mori, Shumpei Yokota, Hirokazu Kimura

Published in: BMC Infectious Diseases | Issue 1/2011

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Abstract

Background

Recent studies strongly suggest that some respiratory viruses are associated with the induction of acute wheezing and/or exacerbation of bronchial asthma. However, molecular epidemiology of these viruses is not exactly known.

Methods

Using PCR technology, we attempted to detect various respiratory viruses from 115 Japanese children. Furthermore, the detected viruses were subjected to homology, pairwise distance, and phylogenetic analysis.

Results

Viruses were detected from 99 (86.1%) patients. Respiratory syncytial virus (RSV) alone and human rhinovirus (HRV) alone were detected in 47 (40.9%) and 36 (31.3%) patients, respectively. Both RSV and HRV were detected in 14 (12.2%) patients. Human metapneumovirus (HMPV) alone and human parainfluenza virus (HPIV) alone were detected in 1 (0.9%) patient each, respectively. Homology and phylogenetic analyses showed that the RSV and HRV strains were classified into genetically diverse species or subgroups. In addition, RSV was the dominant virus detected in patients with no history of wheezing, whereas HRV was dominant in patients with a history of wheezing.

Conclusions

The results suggested that these genetically diverse respiratory viruses, especially RSV and HRV, might be associated with wheezing in Japanese children.
Appendix
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Metadata
Title
A molecular epidemiological study of respiratory viruses detected in Japanese children with acute wheezing illness
Authors
Asako Fujitsuka
Hiroyuki Tsukagoshi
Mika Arakawa
Kazuko Goto-Sugai
Akihide Ryo
Yoshimichi Okayama
Katsumi Mizuta
Atsuyoshi Nishina
Masakazu Yoshizumi
Yoichi Kaburagi
Masahiro Noda
Masato Tashiro
Nobuhiko Okabe
Masaaki Mori
Shumpei Yokota
Hirokazu Kimura
Publication date
01-12-2011
Publisher
BioMed Central
Published in
BMC Infectious Diseases / Issue 1/2011
Electronic ISSN: 1471-2334
DOI
https://doi.org/10.1186/1471-2334-11-168

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