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Molecular Imaging and Biology

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Open Access 01-11-2009 | Research Article

[¹¹C]-DPA-713 and [¹⁸F]-DPA-714 as New PET Tracers for TSPO: A Comparison with [¹¹C]-(R)-PK11195 in a Rat Model of Herpes Encephalitis

Authors: Janine Doorduin, Hans C. Klein, Rudi A. Dierckx, Michelle James, Michael Kassiou, Erik F. J. de Vries

Published in: Molecular Imaging and Biology | Issue 6/2009

Abstract

Background

Activation of microglia cells plays an important role in neurological diseases. Positron emission tomography (PET) with [¹¹C]-(R)-PK11195 has already been used to visualize activated microglia cells in neurological diseases. However, [¹¹C]-(R)-PK11195 may not possess the required sensitivity to visualize mild neuroinflammation. In this study, we evaluated the PET tracers [¹¹C]-DPA-713 and [¹⁸F]-DPA-714 as agents for imaging of activated microglia in a rat model of herpes encephalitis.

Materials and Methods

Rats were intranasally inoculated with HSV-1. On day 6 or 7 after inoculation, small animal PET studies were performed to compare [¹¹C]-(R)-PK11195, [¹¹C]-DPA-713, and [¹⁸F]-DPA-714.

Results

Uptake of [¹¹C]-DPA-713 in infected brain areas was comparable to that of [¹¹C]-(R)-PK11195, but [¹¹C]-DPA-713 showed lower non-specific binding. Non-specific uptake of [¹⁸F]-DPA-714 was lower than that of [¹¹C]-(R)-PK11195. In the infected brain, total [¹⁸F]-DPA-714 uptake was lower than that of [¹¹C]-(R)-PK11195, with comparable specific uptake.

Conclusions

[¹¹C]-DPA-713 may be more suitable for visualizing mild inflammation than [¹¹C]-(R)-PK11195. In addition, the fact that [¹⁸F]-DPA-714 is an agonist PET tracer opens new possibilities to evaluate different aspects of neuroinflammation. Therefore, both tracers warrant further investigation in animal models and in a clinical setting.

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Title: [11C]-DPA-713 and [18F]-DPA-714 as New PET Tracers for TSPO: A Comparison with [11C]-(R)-PK11195 in a Rat Model of Herpes Encephalitis
Authors: Janine Doorduin
Hans C. Klein
Rudi A. Dierckx
Michelle James
Michael Kassiou
Erik F. J. de Vries
Publication date: 01-11-2009
Publisher: Springer-Verlag
Published in: Molecular Imaging and Biology / Issue 6/2009
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-009-0211-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

[¹¹C]-DPA-713 and [¹⁸F]-DPA-714 as New PET Tracers for TSPO: A Comparison with [¹¹C]-(R)-PK11195 in a Rat Model of Herpes Encephalitis

Abstract

Background

Materials and Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Materials and Methods

Results

Conclusions

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