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BMC Complementary Medicine and Therapies

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Open Access 01-12-2021 | Wound Infection | Research article

Identification of components in Kazakhstan honeys that correlate with antimicrobial activity against wound and skin infecting microorganisms

Authors: Pauline McLoone, Aizhan Zhumbayeva, Sofiya Yunussova, Yerkhat Kaliyev, Ludmila Yevstafeva, Susan Verrall, Julie Sungurtas, Ceri Austin, J. Will Allwood, Gordon J. McDougall

Published in: BMC Complementary Medicine and Therapies | Issue 1/2021

Abstract

Background

Antimicrobial drug resistance is a major public health threat that can render infections including wound and skin infections untreatable. The discovery of new antimicrobials is critical. Approaches to discover novel antimicrobial therapies have included investigating the antimicrobial activity of natural sources such as honey. In this study, the anti-microbial activity and chemical composition of 12 honeys from Kazakhstan and medical grade manuka honey were investigated.

Methods

Agar well diffusion and broth culture assays were used to determine anti-microbial activity against a range of skin and wound infecting micro-organisms. Folin-Ciocalteu method was used to determine the total phenol content of the honeys and non-targeted liquid chromatography analysis was performed to identify components that correlated with antimicrobial activity.

Results

In the well diffusion assay, the most susceptible micro-organisms were a clinical isolate of Methicillin resistant Staphylococcus aureus (MRSA) and Enterococcus faecalis (ATCC 19433). Buckwheat & multi-floral honey from Kazakhstan demonstrated the highest antimicrobial activity against these two micro-organisms. Kazakhstan honeys with a buckwheat floral source, and manuka honey had the highest total phenol content. Non-targeted liquid chromatography analysis identified components that correlated with anti-microbial activity as hydroxyphenyl acetic acid, p-coumaric acid, (1H)–quinolinone, and abscisic acid.

Conclusions

The Kazakhstan honeys selected in this study demonstrated antimicrobial activity against wound and skin infecting micro-organisms. Compounds identified as correlating with antimicrobial activity could be considered as potential bioactive agents for the treatment of wound and skin infections.

Available only for authorised users

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Title: Identification of components in Kazakhstan honeys that correlate with antimicrobial activity against wound and skin infecting microorganisms
Authors: Pauline McLoone
Aizhan Zhumbayeva
Sofiya Yunussova
Yerkhat Kaliyev
Ludmila Yevstafeva
Susan Verrall
Julie Sungurtas
Ceri Austin
J. Will Allwood
Gordon J. McDougall
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: Wound Infection
Pseudomonas Aeruginosa
Published in: BMC Complementary Medicine and Therapies / Issue 1/2021
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-021-03466-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Identification of components in Kazakhstan honeys that correlate with antimicrobial activity against wound and skin infecting microorganisms

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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