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Graefe's Archive for Clinical and Experimental Ophthalmology

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Open Access 28-03-2023 | Wound Debridement | Basic Science

A rabbit model for outer retinal atrophy caused by surgical RPE removal

Authors: Sami Al-Nawaiseh, Christina Krötz, Annekatrin Rickmann, Claudine Strack, Anja Germann, Hagen von Briesen, Peter Szurman, André Schulz, Boris V. Stanzel

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 8/2023

Abstract

Purpose

We aimed to establish a rabbit model with retinal atrophy induced by an iatrogenic retinal pigment epithelium (RPE) removal, for future testing of the efficacy and safety of cell therapy strategies.

Methods

A localized detachment of the retina from the RPE/choroid layer was created in 18 pigmented rabbits. The RPE was removed by scraping with a custom-made extendable loop instrument. The resulting RPE wound was observed over a time course of 12 weeks with optical coherence tomography and angiography. After 4 days (group 1) and 12 weeks (group 2), histology was done and staining with hematoxylin and eosin, as well as immunofluorescence performed to further investigate the effects of debridement on the RPE and the overlying retina.

Results

Already after 4 days, we observed a closure of the RPE wound by proliferating RPE and microglia/macrophage cells forming a multilayered clump. This pattern continued over the observation time course of 12 weeks, whereby the inner and outer nuclear layer of the retina became atrophic. No neovascularization was observed in the angiograms or histology. The observed changes were limited to the site of the former RPE wound.

Conclusions

Localized surgical RPE removal induced an adjacent progressive retinal atrophy. Altering the natural course of this model may serve as a basis to test RPE cell therapeutics.

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Title: A rabbit model for outer retinal atrophy caused by surgical RPE removal
Authors: Sami Al-Nawaiseh
Christina Krötz
Annekatrin Rickmann
Claudine Strack
Anja Germann
Hagen von Briesen
Peter Szurman
André Schulz
Boris V. Stanzel
Publication date: 28-03-2023
Publisher: Springer Berlin Heidelberg
Keywords: Wound Debridement
Retinotomy
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 8/2023
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-023-06014-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

A rabbit model for outer retinal atrophy caused by surgical RPE removal

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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