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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2024

Open Access 01-12-2024 | Research

Will Nigella sativa oil protect parotid glands of rats against cranium gamma irradiation? Histological and immunohistochemical evaluation

Authors: Salwa Farid Ahmed, Mostafa A. Bakr

Published in: BMC Complementary Medicine and Therapies | Issue 1/2024

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Abstract

Background

Radiation plays an essential role in treating malignancies. Radiation exposure of salivary glands often results in permanent loss of their functions; therefore, their protection against radiation is crucial. Nigella sativa oil (NSO) is a useful antioxidant against free radicals. The purpose of this study was to investigate the radio-protective effect of NSO on oxidative injury of parotid glands of gamma-irradiated rats. Methods: Twenty-eight male albino rats were divided into four groups (n = 7): Group 1: Neither NSO nor radiation, Group 2: Rats received NSO 400 mg/kg, Group 3: Rats received 15 Gy cranium gamma irradiation & Group 4: Rats received gamma irradiation and NSO. Rats were sacrificed two weeks after the last NSO dose. Histological sections of parotid glands were stained with H&E, Masson’s trichrome and anti-TGF-β antibodies. Area percentage of Masson’s trichrome and TGF-β expression was morphometrically examined. Results: Parotid glands of control and NSO groups revealed normal morphology. Gamma-irradiated glands showed loss of normal acinar architecture and slight acinar shrinkage. NSO treatment of gamma-irradiated glands preserved acinar outline and architecture. Masson’s trichrome stained samples revealed trace amounts of collagen fibers in control and NSO groups, and excessive amounts of collagen fibers in gamma-irradiated group, in addition to few collagen fibers for gamma-irradiated glands treated with NSO. Additionally, control and NSO groups showed negative TGF-β expression. Gamma-irradiated group showed high TGF-β expression, while NSO treated gamma-irradiated group showed moderate TGF-β expression. Conclusions: Gamma-irradiation adversely affected parotid glands, and in contrast, NSO seemed to positively counteract this adverse effect.
Literature
1.
2.
Roach MC, Bradley JD, Robinson CG. Optimizing radiation dose and fractionation for the definitive treatment of locally advanced non-small cell lung cancer. J Thorac Dis. 2018;10(Suppl 21):2465–73.CrossRef
3.
Taysi S, et al. Melatonin reduces oxidative stress in the rat lens due to radiation-induced oxidative injury. Int J Radiat Biol. 2008;84(10):803–8.CrossRefPubMed
4.
Chang DS, Lasley FD, Das IJ, Mendonca MS, Dynlacht JR. Acute effects of total body irradiation (TBI). Basic Radiotherapy Physics and Biology. Cham, Switzerland: Springer; 2014. pp. 259–63.CrossRef
5.
Porter SR, Fedele S, Habbab KM. Xerostomia in head and neck malignancy. Oral Oncol. 2010;46(6):460–3.CrossRefPubMed
6.
Nagler RM. The enigmatic mechanism of irradiation-induced damage to the major salivary glands. Oral Dis. 2002;8:141–6.CrossRefPubMed
7.
Lombaert IM, Brunsting JF, Wierenga PK, Kampinga HH, de Haan G, Coppes RP. Cytokine treatment improves parenchymal and vascular damage of salivary glands after irradiation. Clin Cancer Res. 2008;14:7741–50.CrossRefPubMed
8.
Sumita Y, et al. Bone marrow-derived cells rescue salivary gland function in mice with head and neck irradiation. Int J Biochem Cell Biol. 2011;43:80–7.CrossRefPubMed
9.
Xu L, et al. Resveratrol attenuates radiation-induced salivary gland dysfunction in mice. Laryngoscope. 2013;123:E23–29.CrossRefPubMed
10.
11.
Tembhurne SV, Feroz S, More BH, Sakarkar DM. A review on therapeutic potential of Nigella sativa (kalonji) seeds. J Med Plants Res. 2014;8(3):167–77.CrossRef
12.
Hosseini M, Mohammadpour T, Karami R, Rajaei Z, Reza Sadeghnia H, Soukhtanloo M. Effects of the hydro-alcoholic extract of Nigella sativa on scopolamine-induced spatial memory impairment in rats and its possible mechanism. Chin J Integr Med. 2015;21:438–44.CrossRefPubMed
13.
Beheshti F, et al. Nigella sativa prevented liver and renal tissue damage in lipopolysaccharide-treated rats. Saudi J Kidney Dis Transpl. 2018;29(3):554–66.CrossRefPubMed
14.
Fanoudi S, Alavi MS, Hosseini M, Sadeghnia HR. Nigella sativa and thymoquinone attenuate oxidative stress and cognitive impairment following cerebral hypoperfusion in rats. Metab Brain Dis. 2019;34:1001–10.CrossRefPubMed
15.
Ahlatci A, et al. Radiation-modifying abilities of Nigella sativa and thymoquinone on radiation-induced nitrosative stress in the brain tissue. Phytomedicine. 2014;21(5):740–4.CrossRefPubMed
16.
Assayed ME. Radioprotective effects of black seed (Nigella sativa) oil against hemopoietic damage and immunosuppression in gamma-irradiated rats. Immunopharmacol Immunotoxicol. 2010;32(2):284–96.CrossRefPubMed
17.
Demir E, et al. The effects of Nigella sativa oil, thymoquinone, propolis, and caffeic acid phenethyl ester on radiation-induced cataract. Wien Klin Wochenschr. 2016;128:587–95.CrossRefPubMed
18.
Alkis H, Demir E, Taysi MR, Sagir S, Taysi S. Effects of Nigella sativa oil and thymoquinone on radiation-induced oxidative stress in kidney tissue of rats. Biomed Pharmacother. 2021;139:111540.CrossRefPubMed
19.
Luff M, et al. Nigella sativa oil mitigates xerostomia and preserves salivary function in radiotherapy-treated mice. Laryngoscope Investig Otolaryngol. 2023;8(4):912–20.CrossRefPubMedPubMedCentral
20.
Bayoumi KA, Abdel Fattah A, Gaballah IF. Possible protective potential of atorvastatin and black seed (nigella sativa) oil in amikacin induced nephrotoxicity in adult male albino rats. Egypt J Forensic Appl Toxicol. 2020;20(3):55–65.
21.
De Vasconcelos Catão MH, Nonaka CF, de Albuquerque RL, Bento PM, de Oliveira Costa R. Effects of red laser, infrared, photodynamic therapy, and green LED on the healing process of third-degree burns: clinical and histological study in rats. Lasers Med Sci. 2015;30(1):421–8.CrossRefPubMed
22.
Yang G, Wang Y, Cui Z, Due B, Yin G. Expression and analysis of EPOR after radiation injury of salivary glands in rats. J Odontol. 2018;2(1):101–4.
23.
Singh AK, Pandey P, Tewari M, Pandey HP, Gambhir IS, Shukla HS. Free radicals hasten head and neck cancer risk: a study of total oxidant, total antioxidant, DNA damage, and histological grade. J Postgrad Med. 2016;62(2):96–101.CrossRefPubMedPubMedCentral
24.
Liem IH, et al. Evidence for early and persistent impairment of salivary gland excretion after irradiation of head and neck tumours. Eur J Nucl Med. 1996;23:1485–90.CrossRefPubMed
25.
Valdez IH, Atkinson JC, Ship JA, Fox PC. Major salivary gland function in patients with radiation-induced xerostomia: flow rates and sialochemistry. Int J Radiat Oncol Biol Phys. 1993;25(1):41–7.CrossRefPubMed
26.
Coppes RP, Vissink A, Konings AW. Comparison of radiosensitivity of rat parotid and submandibular glands after different radiation schedules. Radiother Oncol. 2002;63(3):321–8.CrossRefPubMed
27.
Gomes CC, Ramos-Perez FM, Perez DE, Novaes PD, Bóscolo FN, Almeida SM. Radioprotective effect of vitamin E in parotid glands: a morphometric analysis in rats. Braz Dent J. 2013;24:183–7.CrossRefPubMed
28.
29.
30.
Xu L, et al. Simvastatin attenuates radiation-induced salivary gland dysfunction in mice. Drug Des Devel Ther. 2016;13:2271–8.ADS
31.
Nam K, et al. Post-irradiated human submandibular glands display high collagen deposition, disorganized cell junctions, and an increased number of adipocytes. J Histochem Cytochem. 2016;64(6):343–52.CrossRefPubMedPubMedCentral
32.
Nor-Eldin EK, Elsayed HM. The possible radioprotective effects of vitamin E, Nigella sativa oil, and melatonin against X-ray induced early acute changes in cerebral and cerebellar cortices in albino rats: histological and Immunohistochemical. Egypt J Histol. 2019;42(4):767–82.
33.
Korany NS, Ezzat BA. Prophylactic effect of green tea and Nigella sativa extracts against fenitrothion-induced toxicity in rat parotid gland. Arch Oral Biol. 2011;56(11):1339–46.CrossRefPubMed
34.
Mouket S, Demir E, Yucel A, Taysi S. Nigella sativa oil reduces oxidative/nitrosative stress in the salivary gland of rats exposed to total cranial irradiation. Drug Chem Toxicol. 2022;20:1–6.
35.
Kaplan M, et al. Radioprotective effect of nigella sativa oil on heart tissues of rats exposed to XXXerostomiaXXX. Int J Cardiovasc Sci. 2022;35:214–9.
36.
Shahzad M, et al. Suppressive effects of black seed oil on ovalbumin induced acute lung remodelling in E3 rats. Swiss Med Wkly. 2010;140(4950):w13128.PubMed
37.
Majdalawieh AF, Hmaidan R, Carr RI. Nigella sativa modulates splenocyte proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity. J Ethnopharmacol. 2010;131(2):268–75.CrossRefPubMed
38.
Umar S, Zargan J, Umar K, Ahmad S, Katiyar CK, Khan HA. Modulation of the oxidative stress and inflammatory cytokine response by thymoquinone in the collagen induced arthritis in Wistar rats. Chem Biol Interact. 2012;197(1):40–6.CrossRefPubMed
39.
Amin SM, et al. Radioprotective effect of Nigella sativa Oil (NSO) against radiation-induced hepatic toxicity and haematological alteration in irradiated albino mice. Int J Radiat Res. 2023;21(1):89–95.
40.
Üstün K, et al. Radio-protective effects of Nigella sativa oil on oxidative stress in tongue tissue of rats. Oral Dis. 2014;20(1):109–13.CrossRefPubMed
Metadata
Title
Will Nigella sativa oil protect parotid glands of rats against cranium gamma irradiation? Histological and immunohistochemical evaluation
Authors
Salwa Farid Ahmed
Mostafa A. Bakr
Publication date
01-12-2024
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2024
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-024-04410-8

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