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01-12-2018 | Original Paper

Validation and application of a novel APC antibody in western blotting, immunoprecipitation, and immunohistochemistry

Authors: Nami O. Yamada, Wenduerma, Shuji Matsuda, Takao Senda

Published in: Medical Molecular Morphology | Issue 4/2018

Abstract

Adenomatous polyposis coli (APC) is a large protein with multiple binding partners, suggesting diverse functions besides its well-known role in the destruction of β-catenin. To elucidate these complex functions, it is crucial to evaluate the precise subcellular distribution of APC within a cell and tissue. However, most of the commercially available anti-APC antibodies can only be used for limited applications, resulting in the use of independently generated antibodies. This has led to various discrepancies between studies as a common antibody has not been established. In this study, we generated an antibody against the c-terminal domain of human APC, designated APC-C antibody, and evaluated its specificity and application in various immunological methods. Our data indicate that this novel APC-C antibody is a specific and versatile antibody that can be used in western blotting, immunoprecipitation, immunocytochemistry, and immunohistochemistry. Widespread use of this APC antibody will help enhance our understanding of APC’s function in both normal and cancer cell biology.

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Title: Validation and application of a novel APC antibody in western blotting, immunoprecipitation, and immunohistochemistry
Authors: Nami O. Yamada
Wenduerma
Shuji Matsuda
Takao Senda
Publication date: 01-12-2018
Publisher: Springer Japan
Published in: Medical Molecular Morphology / Issue 4/2018
Print ISSN: 1860-1480
Electronic ISSN: 1860-1499
DOI: https://doi.org/10.1007/s00795-018-0196-9

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Validation and application of a novel APC antibody in western blotting, immunoprecipitation, and immunohistochemistry

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