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01-09-2017 | Original Article

Utility of PAS and β-catenin staining in histological categorisation and prediction of prognosis of hepatoblastomas

Authors: Goutam Bera, Ram Narayan Das, Paromita Roy, Ranajoy Ghosh, Nelofar Islam, Prafulla Kumar Mishra, Uttara Chatterjee

Published in: Pediatric Surgery International | Issue 9/2017

Abstract

Aims

The aim of this study was to assess the usefulness of PAS, β-catenin and Ki-67 in categorising hepatoblastomas (HBs) and their significance in predicting prognosis. In addition, we have also documented the various chemotherapy induced histologic changes in HBs.

Method

In this retrospective observational study of 29 cases of hepatoblastomas, 27 cases were considered for statistical analysis, excluding two cases of diagnostic core biopsies. All clinicopathological parameters and follow up data were collected. All HB cases including the mixed epithelial and mesenchymal HBs were classified into two groups: fetal predominant and embryonal predominant type according to the predominant epithelial component. PAS, β-catenin and Ki-67 staining were done and their correlation with histologic subtypes was assessed. Kaplan–Meier survival analysis was performed in relation to histology, PAS, β-catenin and Ki-67 staining characteristics.

Result

Diffuse nuclear staining of β-catenin was significantly more common in embryonal predominant type (p = 0.036), whereas strong PAS positivity was significantly associated with fetal predominant type (p = 0.002). But no significant correlation was seen between histologic type and Ki-67 staining (p = 0.42). Survival analysis showed cases with diffuse PAS positivity, focal nuclear β-catenin staining and low Ki-67 LI had better survival.

Conclusion

PAS is a simple stain and can be utilised in histological categorisation of HB and also predicting its outcome. Nuclear β-catenin staining which is significantly common in embryonal elements in HB predicts shorter survival.

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Title: Utility of PAS and β-catenin staining in histological categorisation and prediction of prognosis of hepatoblastomas
Authors: Goutam Bera
Ram Narayan Das
Paromita Roy
Ranajoy Ghosh
Nelofar Islam
Prafulla Kumar Mishra
Uttara Chatterjee
Publication date: 01-09-2017
Publisher: Springer Berlin Heidelberg
Published in: Pediatric Surgery International / Issue 9/2017
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-017-4115-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Utility of PAS and β-catenin staining in histological categorisation and prediction of prognosis of hepatoblastomas

Abstract

Aims

Method

Result

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Aims

Method

Result

Conclusion

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