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Pediatric Nephrology
Published in: Pediatric Nephrology 10/2017

01-10-2017 | Original Article

Urinary podocalyxin as a possible novel marker of intrauterine nephrogenesis and extrauterine podocyte injury

Authors: Taihei Hayashi, Shuko Tokuriki, Takashi Okuno, Genrei Ohta, Aiko Igarashi, Yusei Ohshima

Published in: Pediatric Nephrology | Issue 10/2017

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Abstract

Background

The number of nephrons at birth is determined during fetal development and is modulated thereafter by postnatal podocyte injury. Hyperfiltration, caused by a reduced number of nephrons, is a risk factor for chronic kidney disease. It is therefore important to monitor the formation of nephrons.

Methods

Urine samples were collected from infants within 1–2 days of birth, with follow-up sampling for preterm infants at 37–39 weeks of corrected age. Urinary levels of podocalyxin (PCX), β2-microglobulin (β2MG), N-acetyl-ß-D-glucosaminidase (NAG), total protein (TP), microalbumin (mAlb) and creatinine were measured and the relationship between these markers evaluated.

Results

Seventy-nine neonates were enrolled in this study. Urinary levels of PCX at birth were higher than normal adult reference values, with levels increasing up to a gestational age of 36 weeks (p = 0.0242). At 37–39 weeks corrected age, urinary levels of PCX decreased to adult levels. The levels of PCX in the urine at birth were not correlated to urinary levels of β2MG, NAG, TP and mAlb.

Conclusions

An increased urinary level of PCX may be a marker of both active nephron formation and podocyte injury sustained at birth. As such, changes in urinary levels of PCX are likely to reflect adaptation of renal function to the extra-uterine environment.
Literature
1.
Carmody JB, Charlton JR (2013) Short-term gestation, long-term risk: prematurity and chronic kidney disease. Pediatrics 131:1168–1179CrossRefPubMed
2.
Barker DJ, Osmond C (1986) Infant mortality, childhood nutrition, and ischaemic heart disease in England and Wales. Lancet 1:1077–1081CrossRefPubMed
3.
Hinchliffe SA, Sargent PH, Howard CV, Chan YF, van Velzen D (1991) Human intrauterine renal growth expressed in absolute number of glomeruli assessed by the disector method and Cavalieri principle. Lab Investig 64:777–784PubMed
4.
Hodgin JB, Rasoulpour M, Markowitz GS, D’Agati VD (2009) Very low birth weight is a risk factor for secondary focal segmental glomerulosclerosis. Clin J Am Soc Nephrol 4:71–76CrossRefPubMedPubMedCentral
5.
Ikezumi Y, Suzuki T, Karasawa T, Yamada T, Hasegawa H, Nishimura H, Uchiyama M (2013) Low birthweight and premature birth are risk factors for podocytopenia and focal segmental glomerulosclerosis. Am J Nephrol 38:149–157CrossRefPubMed
6.
Vikse BE, Irgens LM, Leivestad T, Hallan S, Iversen BM (2008) Low birth weight increases risk for end-stage renal disease. J Am Soc Nephrol 19:151–157CrossRefPubMedPubMedCentral
7.
White SL, Perkovic V, Cass A, Chang CL, Poulter NR, Spector T, Haysom L, Craig JC, Salmi IA, Chadban SJ, Huxley RR (2009) Is low birth weight an antecedent of CKD in later life? A systematic review of observational studies. Am J Kidney Dis 54:248–261CrossRefPubMed
8.
9.
Mundel P, Shankland SJ (2002) Podocyte biology and response to injury. J Am Soc Nephrol 13:3005–3015CrossRefPubMed
10.
Yu D, Petermann A, Kunter U, Rong S, Shankland SJ, Floege J (2005) Urinary podocyte loss is a more specific marker of ongoing glomerular damage than proteinuria. J Am Soc Nephrol 16:1733–1741CrossRefPubMed
11.
Kent AL, Brown L, Broom M, Broomfield A, Dahlstrom JE (2012) Increased urinary podocytes following indomethacin suggests drug-induced glomerular injury. Pediatr Nephrol 27:1111–1117CrossRefPubMed
12.
Kerjaschki D, Sharkey DJ, Farquhar MG (1984) Identification and characterization of podocalyxin—the major sialoprotein of the renal glomerular epithelial cell. J Cell Biol 98:1591–1596CrossRefPubMed
13.
Nielsen JS, McNagny KM (2009) The role of podocalyxin in health and disease. J Am Soc Nephrol 20:1669–1676CrossRefPubMed
14.
15.
Kanda Y (2013) Investigation of the freely available easy-to-use software ‘EZR’ for medical statistics. Bone Marrow Transplant 48:452–458CrossRefPubMed
16.
Hara M, Yamagata K, Tomino Y, Saito A, Hirayama Y, Ogasawara S, Kurosawa H, Sekine S, Yan K (2012) Urinary podocalyxin is an early marker for podocyte injury in patients with diabetes: establishment of a highly sensitive ELISA to detect urinary podocalyxin. Diabetologia 55:2913–2919CrossRefPubMedPubMedCentral
17.
Langhendries JP, Gillain N, Battisti O, Carlier B, Bertrand JM (1987) Normal values for urinary N-acetyl-beta-glucosaminidase excretion in preterm and term babies. Arch Dis Child 62:483–486CrossRefPubMedPubMedCentral
18.
Gubhaju L, Sutherland MR, Horne RS, Medhurst A, Kent AL, Ramsden A, Moore L, Singh G, Hoy WE, Black MJ (2014) Assessment of renal functional maturation and injury in preterm neonates during the first month of life. Am J Physiol Ren Physiol 307:F149–F158CrossRef
19.
Kanwar YS, Wada J, Lin S, Danesh FR, Chugh SS, Yang Q, Banerjee T, Lomasney JW (2004) Update of extracellular matrix, its receptors, and cell adhesion molecules in mammalian nephrogenesis. Am J Physiol Ren Physiol 286:F202–F215CrossRef
20.
Fernandez D, Larrucea S, Nowakowski A, Pericacho M, Parrilla R, Ayuso MS (2011) Release of podocalyxin into the extracellular space. Role of metalloproteinases. Biochim Biophys Acta 1813:1504–1510CrossRefPubMed
21.
Toth-Heyn P, Drukker A, Guignard JP (2000) The stressed neonatal kidney: from pathophysiology to clinical management of neonatal vasomotor nephropathy. Pediatr Nephrol 14:227–239CrossRefPubMed
22.
El Hamel C, Chianea T, Thon S, Lepichoux A, Yardin C, Guigonis V (2016) Normal values of urine total protein- and albumin-to-creatinine ratios in term newborns. Pediatr Nephrol 32:113–118CrossRefPubMed
23.
24.
Rodriguez MM, Gomez AH, Abitbol CL, Chandar JJ, Duara S, Zilleruelo GE (2004) Histomorphometric analysis of postnatal glomerulogenesis in extremely preterm infants. Pediatr Dev Pathol 7:17–25CrossRefPubMed
Metadata
Title
Urinary podocalyxin as a possible novel marker of intrauterine nephrogenesis and extrauterine podocyte injury
Authors
Taihei Hayashi
Shuko Tokuriki
Takashi Okuno
Genrei Ohta
Aiko Igarashi
Yusei Ohshima
Publication date
01-10-2017
Publisher
Springer Berlin Heidelberg
Published in
Pediatric Nephrology / Issue 10/2017
Print ISSN: 0931-041X
Electronic ISSN: 1432-198X
DOI
https://doi.org/10.1007/s00467-017-3676-6

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