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Open Access 01-05-2012 | General Gynecology

Up-regulation of inhibitors of DNA binding/differentiation gene during alendronate-induced osteoblast differentiation

Authors: Ae Ra Kang, Young Rim Oh, Heung Yeol Kim, Min Jung Park, Bo Sun Joo, Won Jun Choi, Ji Young Lee, Min Hyung Jung, Yong Il Ji, Jong Soon Choi

Published in: Archives of Gynecology and Obstetrics | Issue 5/2012

Abstract

Purpose

To investigate the effect of alendronate on the expression of Id genes in osteoblast differentiation.

Methods

C2C12 cells were treated with alendronate for various concentrations and time periods. For evaluation of alendronate-induced osteoblast differentiation in C2C12 cells, alkaline phosphatase (ALP) activity was measured. The expression of osteoblast differentiation markers such as ALP, type-1 collagen (Col 1), and osteocalcin (OCN), and the expression of Id-1 and Id-2 were measured by RT-PCR. In order to understand the mechanism underlying the regulation of Id genes, the promoter region of the Id-1 gene was identified. Database analysis of the promoter region for Id-1 using known consensus sequences identified several putative response elements, including CCAAT/enhancer-binding protein beta (C/EBPβ).

Results

Alendronate treatment significantly increased not only ALP activity but also the expression of ALP, Col 1, and OCN, Id-1 and Id-2. C/EBPβ and alendronate cooperatively increased the promoter activity and expression of Id-1.

Conclusions

These results suggest that C/EBPβ-mediated Id-1 transcriptional activation may regulate alendronate-induced osteoblast differentiation of C2C12 cells.

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Title: Up-regulation of inhibitors of DNA binding/differentiation gene during alendronate-induced osteoblast differentiation
Authors: Ae Ra Kang
Young Rim Oh
Heung Yeol Kim
Min Jung Park
Bo Sun Joo
Won Jun Choi
Ji Young Lee
Min Hyung Jung
Yong Il Ji
Jong Soon Choi
Publication date: 01-05-2012
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 5/2012
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-011-2141-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Up-regulation of inhibitors of DNA binding/differentiation gene during alendronate-induced osteoblast differentiation

Abstract

Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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