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Journal of Experimental & Clinical Cancer Research
Published in: Journal of Experimental & Clinical Cancer Research 1/2009

Open Access 01-01-2009 | Research

Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer

Authors: Chunling Huang, Xianming Liang, Ruxin Huang, Zhongying Zhang

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2009

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Abstract

Background

This study aimed to find novel biomarkers for colorectal cancer.

Methods

Fluorescent mRNA differential display PCR (DD-PCR) was used to screen the genes differentially expressed in colorectal cancer tissues and their adjacent tissues. The differentially expressed genes were confirmed by real-time PCR and then their clinical relevance (such as association with tumor location and lymph gland metastasis) was further investigated.

Results

We identified by DD-PCR a novel RNA helicase, DHX32, which showed higher expression in colorectal cancer tissues than their adjacent tissues, and this result was confirmed by real time RT-PCR. In addition, we found that the level of DHX32 gene expression in colorectal cancer was significantly associated with cancer location, lymph gland metastasis, cancer nodal status, differentiation grade, and Dukes, stage.

Conclusion

DHX32 may play an important role in the development of colorectal cancer and could serve as a novel biomarker for colorectal cancer after additional investigation.
Appendix
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Metadata
Title
Up-regulation and clinical relevance of novel helicase homologue DHX32 in colorectal cancer
Authors
Chunling Huang
Xianming Liang
Ruxin Huang
Zhongying Zhang
Publication date
01-01-2009
Publisher
BioMed Central
Published in
Journal of Experimental & Clinical Cancer Research / Issue 1/2009
Electronic ISSN: 1756-9966
DOI
https://doi.org/10.1186/1756-9966-28-11

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