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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 5/2019

01-05-2019 | Gamete Biology

Understanding mechanisms of oocyte development by follicular fluid lipidomics

Authors: Daniela Antunes Montani, Daniela Paes de Almeida Ferreira Braga, Edson Borges Jr, Mariana Camargo, Fernanda Bertuccez Cordeiro, Eduardo Jorge Pilau, Fábio Cesar Gozzo, Renato Fraietta, Edson Guimarães Lo Turco

Published in: Journal of Assisted Reproduction and Genetics | Issue 5/2019

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Abstract

Purpose

The present study aimed to provide a non-invasive approach to studying mechanisms responsible for oocyte development.

Methods

To this end, follicular fluid (FF) from 62 patients undergoing in vitro fertilization (IVF) cycles was split into two groups depending on the pregnancy outcome: pregnant (n = 28) and non-pregnant (n = 34) groups. Data were acquired by the MALDI-TOF mass spectrometry. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to the data set. A ROC curve, to predict success rate, was constructed, and the lipids were attributed.

Results

Six ions were differentially represented in FF of pregnant and non-pregnant patients, with an area under the curve of 0.962. Phosphatidic acid, phosphatidylglycerol, and triacylglycerol were hyper-represented in the pregnant group, while glucosylceramide was hyper-represented in the non-pregnant group. Enriched functions related to these lipids are steroidogenesis, cellular response, signal transduction, cell cycle, and activation of protein kinase C for the pregnant group and apoptosis inhibition for the non-pregnant group.

Conclusion

Human FF fingerprinting can both improve the understanding concerning mechanisms responsible for oocyte development and its effect on embryo implantation potential and assist in the management of IVF cycles.
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Metadata
Title
Understanding mechanisms of oocyte development by follicular fluid lipidomics
Authors
Daniela Antunes Montani
Daniela Paes de Almeida Ferreira Braga
Edson Borges Jr
Mariana Camargo
Fernanda Bertuccez Cordeiro
Eduardo Jorge Pilau
Fábio Cesar Gozzo
Renato Fraietta
Edson Guimarães Lo Turco
Publication date
01-05-2019
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 5/2019
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-019-01428-7

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