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Inflammation Research
Published in: Inflammation Research 9/2012

01-09-2012 | Original Research Paper

Two circulating neutrophil populations in acute inflammation in mice

Authors: Hildur H. Arnardottir, Jona Freysdottir, Ingibjorg Hardardottir

Published in: Inflammation Research | Issue 9/2012

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Abstract

Objective and design

Recent studies indicate that neutrophils are heterogeneous and may have an immunosuppressive role in addition to their well-known phagocytic and bactericidal function. This study examined neutrophil subpopulations in the circulation, peritoneum, spleen and bone marrow from mice at various time points after induction of acute inflammation.

Material, treatment and methods

Female C57BL/6 mice were injected intraperitoneally with lipopolysaccharide (LPS). Blood, peritoneal, spleen and bone marrow cells were collected and counted and expression of surface molecules and chemokine receptors analyzed with flow cytometry. Chemokine and cytokine concentrations in serum and peritoneal fluid were determined by ELISA.

Results

Neutrophil numbers in the circulation decreased following administration of LPS but reached similar numbers to those prior to inflammation at 8 h. At that time point, two distinct neutrophil populations were present in the circulation. These two neutrophil populations differed in size, granularity and expression of CD11b and Ly6G. Few neutrophils were recruited into the peritoneum until 24 h after administration of LPS at a time when the neutrophils in the circulation had increased their expression of the chemokine receptor CXCR2.

Conclusions

Induction of acute inflammation leads to the appearance of two circulating neutrophil subpopulations, which may differ in their activation state and function.
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Metadata
Title
Two circulating neutrophil populations in acute inflammation in mice
Authors
Hildur H. Arnardottir
Jona Freysdottir
Ingibjorg Hardardottir
Publication date
01-09-2012
Publisher
SP Birkhäuser Verlag Basel
Published in
Inflammation Research / Issue 9/2012
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-012-0484-0

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