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01-10-2011 | Original Article

Trichostatin A inhibits expression of cathepsins in experimental osteoarthritis

Authors: Wei-Ping Chen, Jia-Peng Bao, Jin-Li Tang, Peng-Fei Hu, Li-Dong Wu

Published in: Rheumatology International | Issue 10/2011

Abstract

The aim of this study was to investigate the effects of trichostatin A (TSA) on expression of cathepsins in cartilage in experimental osteoarthritis (OA). OA was induced in 18 rabbits by bilateral anterior cruciate ligament transection (ACLT). Four weeks after surgery, rabbits received intra-articular injection with TSA dissolved in the dimethylsulphoxide (DMSO) in the right knees and DMSO in the left knees once a week for 5 weeks. Rabbits were killed 7 days after the last injection. The knee joints were assessed by morphological and histological examination. Messenger RNA expression of cathepsins K, B, L, S and cystatin C was studied by real-time PCR. TSA inhibited the expression of cathepsins K, B, L, S and cystatin C accompanied with the less degradation in cartilage. The results suggest that TSA exhibits protective effects against cartilage degradation in rabbits with OA and the effects may be associated with the inhibition of cathepsins.

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Title: Trichostatin A inhibits expression of cathepsins in experimental osteoarthritis
Authors: Wei-Ping Chen
Jia-Peng Bao
Jin-Li Tang
Peng-Fei Hu
Li-Dong Wu
Publication date: 01-10-2011
Publisher: Springer-Verlag
Published in: Rheumatology International / Issue 10/2011
Print ISSN: 0172-8172
Electronic ISSN: 1437-160X
DOI: https://doi.org/10.1007/s00296-010-1481-7

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