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Journal of Orthopaedic Science

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Open Access 01-07-2013 | Original Article

Treatment of cartilage defects by subchondral drilling combined with covering with atelocollagen membrane induces osteogenesis in a rat model

Authors: Michio Hamanishi, Tomoyuki Nakasa, Naosuke Kamei, Hiromi Kazusa, Goki Kamei, Mitsuo Ochi

Published in: Journal of Orthopaedic Science | Issue 4/2013

Abstract

Background

The coverage of the atelocollagen membrane at the chondral defect after subchondral drilling might improve the beneficial effects for cartilage repair because of the prevention of scattering and accumulation of cells and growth factors from bone marrow within the chondral defect. On the other hand, it might block cells and factors derived from the synovium or cause high pressure in the chondral defect, resulting in prevention of cells and growth factors gushing out from the bone marrow, which leads to disadvantages for cartilage repair.

Method

We tested this hypothesis in a 2-mm-diameter chondral defect created in the articular cartilage of the patellar groove in a rat models. Defects were left untreated, or were drilled or drilled and covered with an atelocollagen membrane; healing was evaluated by histology and gene expression analysis using real-time polymerase chain reaction and immunohistochemistry.

Results

Membrane coverage induced bone tissue ingrowth into the punched chondral defect. At 1 week, expression of TGFβ, Sox9, Runx2, osteocalcin, Col1a1, and Col2a1 in the drilling group was significantly higher than in the covering group. At 4 weeks, expressions of TGFβ, Runx2, and Col1a1 were all significantly higher in the drilling group, while Sox9, osteocalcin, and Col2a1 were significantly higher in the covering group. Immunohistochemistry demonstrated Sox9, osteocalcin, and type II collagen on the bony reparative tissue in the covering group.

Conclusions

These results suggest that the atelocollagen membrane coverage resulted in inhibition of cartilage repair.

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Title: Treatment of cartilage defects by subchondral drilling combined with covering with atelocollagen membrane induces osteogenesis in a rat model
Authors: Michio Hamanishi
Tomoyuki Nakasa
Naosuke Kamei
Hiromi Kazusa
Goki Kamei
Mitsuo Ochi
Publication date: 01-07-2013
Publisher: Springer Japan
Published in: Journal of Orthopaedic Science / Issue 4/2013
Print ISSN: 0949-2658
Electronic ISSN: 1436-2023
DOI: https://doi.org/10.1007/s00776-013-0379-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Treatment of cartilage defects by subchondral drilling combined with covering with atelocollagen membrane induces osteogenesis in a rat model

Abstract

Background

Method

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Method

Results

Conclusions

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