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01-07-2017 | Original Article

Transformed Root Extract of Leonurus sibiricus Induces Apoptosis through Intrinsic and Extrinsic Pathways in Various Grades of Human Glioma Cells

Authors: Przemysław Sitarek, Ewa Skała, Monika Toma, Marzena Wielanek, Janusz Szemraj, Tomasz Skorski, Adam J. Białas, Tomasz Sakowicz, Tomasz Kowalczyk, Maciej Radek, Halina Wysokińska, Tomasz Śliwiński

Published in: Pathology & Oncology Research | Issue 3/2017

Abstract

This study determines the influence of transformed root (TR) extract of Leonurus sibiricus L. on various grades (I-III) of human glioma cells derived from patients. This plant occurs in southern Asia and Siberia and is widely used as a medicinal plant with various biological activities. Chromatographic profile of TR extract have revealed the presence of various polyphenolic compounds (4-hydroxybenzoic acid, gentisic acid, vanilic acid, 1,3-dicaffeoylquinic acid, α-resorcylic acid). We found TR root extract to have antiproliferative activity on glioma cells after 24 h of treatment. TR root extract induces apoptosis on various grades (I-III) of human glioma cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, enhanced S and G2/M phases of the cell cycle, and altered mRNA levels of Bax, Bcl-2, p53, Cas-3, Cas-8 and Cas-9 factors involved in apoptosis. This work for the first time demonstrate that TR extract from L. sibiricus root has the potential to activate apoptosis in grade I-III human glioma cells through the intrinsic and extrinsic pathways.

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Title: Transformed Root Extract of Leonurus sibiricus Induces Apoptosis through Intrinsic and Extrinsic Pathways in Various Grades of Human Glioma Cells
Authors: Przemysław Sitarek
Ewa Skała
Monika Toma
Marzena Wielanek
Janusz Szemraj
Tomasz Skorski
Adam J. Białas
Tomasz Sakowicz
Tomasz Kowalczyk
Maciej Radek
Halina Wysokińska
Tomasz Śliwiński
Publication date: 01-07-2017
Publisher: Springer Netherlands
Published in: Pathology & Oncology Research / Issue 3/2017
Print ISSN: 1219-4956
Electronic ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-016-0170-6

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