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Acta Neuropathologica Communications
Published in: Acta Neuropathologica Communications 1/2015

Open Access 01-12-2015 | Research

Post-mortem brain analyses of the Lothian Birth Cohort 1936: extending lifetime cognitive and brain phenotyping to the level of the synapse

Authors: Christopher M. Henstridge, Rosemary J. Jackson, JeeSoo M. Kim, Abigail G. Herrmann, Ann K. Wright, Sarah E. Harris, Mark E. Bastin, John M. Starr, Joanna Wardlaw, Thomas H. Gillingwater, Colin Smith, Chris-Anne McKenzie, Simon R. Cox, Ian J. Deary, Tara L. Spires-Jones

Published in: Acta Neuropathologica Communications | Issue 1/2015

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Abstract

Introduction

Non-pathological, age-related cognitive decline varies markedly between individuals and places significant financial and emotional strain on people, their families and society as a whole. Understanding the differential age-related decline in brain function is critical not only for the development of therapeutics to prolong cognitive health into old age, but also to gain insight into pathological ageing such as Alzheimer’s disease. The Lothian Birth Cohort of 1936 (LBC1936) comprises a rare group of people for whom there are childhood cognitive test scores and longitudinal cognitive data during older age, detailed structural brain MRI, genome-wide genotyping, and a multitude of other biological, psycho-social, and epidemiological data. Synaptic integrity is a strong indicator of cognitive health in the human brain; however, until recently, it was prohibitively difficult to perform detailed analyses of synaptic and axonal structure in human tissue sections. We have adapted a novel method of tissue preparation at autopsy to allow the study of human synapses from the LBC1936 cohort in unprecedented morphological and molecular detail, using the high-resolution imaging techniques of array tomography and electron microscopy. This allows us to analyze the brain at sub-micron resolution to assess density, protein composition and health of synapses. Here we present data from the first donated LBC1936 brain and compare our findings to Alzheimer’s diseased tissue to highlight the differences between healthy and pathological brain ageing.

Results

Our data indicates that compared to an Alzheimer’s disease patient, the cognitively normal LBC1936 participant had a remarkable degree of preservation of synaptic structures. However, morphological and molecular markers of degeneration in areas of the brain associated with cognition (prefrontal cortex, anterior cingulate cortex, and superior temporal gyrus) were observed.

Conclusions

Our novel post-mortem protocol facilitates high-resolution neuropathological analysis of the well-characterized LBC1936 cohort, extending phenotyping beyond cognition and in vivo imaging to now include neuro pathological changes, at the level of single synapses. This approach offers an unprecedented opportunity to study synaptic and axonal integrity during ageing and how it contributes to differences in age-related cognitive change.
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Metadata
Title
Post-mortem brain analyses of the Lothian Birth Cohort 1936: extending lifetime cognitive and brain phenotyping to the level of the synapse
Authors
Christopher M. Henstridge
Rosemary J. Jackson
JeeSoo M. Kim
Abigail G. Herrmann
Ann K. Wright
Sarah E. Harris
Mark E. Bastin
John M. Starr
Joanna Wardlaw
Thomas H. Gillingwater
Colin Smith
Chris-Anne McKenzie
Simon R. Cox
Ian J. Deary
Tara L. Spires-Jones
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Acta Neuropathologica Communications / Issue 1/2015
Electronic ISSN: 2051-5960
DOI
https://doi.org/10.1186/s40478-015-0232-0

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