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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2016

Open Access 01-12-2016 | Research article

A new model of implant-related osteomyelitis in the metaphysis of rat tibiae

Authors: Norbert Harrasser, Johannes Gorkotte, Andreas Obermeier, Susanne Feihl, Melanie Straub, Julia Slotta-Huspenina, Ruediger von Eisenhart-Rothe, Walter Moser, Philipp Gruner, Michael de Wild, Hans Gollwitzer, Rainer Burgkart

Published in: BMC Musculoskeletal Disorders | Issue 1/2016

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Abstract

Background

Animal models serve as an important tool to understand peri-implant infection. Most of the models use high bacterial loads (>104 colony forming units, CFU) to provide high infection rates. Therefore these animals evolve rather similarly, making comparison between groups and statistical analysis possible. On the other hand, to mimic clinical constellation of surgery-related infections the use of low amounts of bacteria would be more advantageous.

Methods

We developed a metaphyseal rat model of peri-implant bone infection with low amount of bacterial loads (102 and 103 CFU of Staphylococcus aureus) and investigated osseointegration of the implants coated with hydroxyapatite (HA) and low-dosed HA-silver (HA-Ag). Non-infected implants served as controls. After 6 weeks rats were sacrificed and implants evaluated for osseointegration and infection.

Results

Infection of implanted devices was reliably induced, independently whether 102 or 103 CFU of S. aureus were inoculated and HA or HA-Ag coated implants were used. No systemic infection was present in any of the animals at the time of sacrifice, and no animal developed acute infection requiring premature sacrifice. All CFU counts of the implant and the bone at sacrifice were significantly higher than the inoculated load (p < .05). All sterilely inserted implants showed excellent osseointegration and no infection.

Conclusions

Our present study of a rat tibia model reliably induced osteomyelitis in the metaphysis with low-doses of bacteria. The addition of low-dosed Ag to the implant coating was not able to reduce the infection rates. The results demonstrate that it is possible to develop a model of implant-related osteomyelitis in rats with low amounts of bacteria to better mimic clinical constellations. No other promoters of infection besides insertion of the screw implant were used in this model.
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Metadata
Title
A new model of implant-related osteomyelitis in the metaphysis of rat tibiae
Authors
Norbert Harrasser
Johannes Gorkotte
Andreas Obermeier
Susanne Feihl
Melanie Straub
Julia Slotta-Huspenina
Ruediger von Eisenhart-Rothe
Walter Moser
Philipp Gruner
Michael de Wild
Hans Gollwitzer
Rainer Burgkart
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2016
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-016-1005-z

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