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European Archives of Oto-Rhino-Laryngology

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Open Access 01-05-2018 | Laryngology

Towards an in vitro fibrogenesis model of human vocal fold scarring

Authors: M. Graupp, B. Rinner, M. T. Frisch, G. Weiss, J. Fuchs, M. Sundl, A. El-Heliebi, G. Moser, L. P. Kamolz, M. Karbiener, M. Gugatschka

Published in: European Archives of Oto-Rhino-Laryngology | Issue 5/2018

Abstract

Background

Vocal fold (VF) scarring remains a therapeutic dilemma and challenge in modern laryngology. To facilitate corresponding research, we aimed to establish an in vitro fibrogenesis model employing human VF fibroblasts (hVFF) and the principles of macromolecular crowding (MMC).

Methods

Fibrogenesis was promoted by addition of transforming growth factor-β1 to standard medium and medium containing inert macromolecules (MMC). Hepatocyte growth factor (HGF) and Botox type A were tested for their antifibrotic properties in various doses. Experiments were analyzed with respect to the biosynthesis of collagen, fibronectin, and α-smooth muscle actin using immunofluorescence, silver stain and western blot.

Results

MMC led to favourable enhanced deposition of collagen and other extracellular matrix components, reflecting fibrotic conditions. Low doses of HGF were able to dampen profibrotic effects. This could not be observed for higher HGF concentrations. Botox type A did not show any effects.

Conclusion

Based on the principles of MMC we could successfully establish a laryngeal fibrogenesis model employing hVFF. Our finding of dose-dependent HGF effects is important before going into clinical trials in humans and has never been shown before. Our model provides a novel option to screen various potential antifibrotic compounds under standardized conditions in a short time.

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Title: Towards an in vitro fibrogenesis model of human vocal fold scarring
Authors: M. Graupp
B. Rinner
M. T. Frisch
G. Weiss
J. Fuchs
M. Sundl
A. El-Heliebi
G. Moser
L. P. Kamolz
M. Karbiener
M. Gugatschka
Publication date: 01-05-2018
Publisher: Springer Berlin Heidelberg
Published in: European Archives of Oto-Rhino-Laryngology / Issue 5/2018
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-018-4922-7

2024 ASCO Annual Meeting

Springer Medicine

Towards an in vitro fibrogenesis model of human vocal fold scarring

Abstract

Background

Methods

Results

Conclusion

2024 ASCO Annual Meeting

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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