Top

Published in:

01-06-2019 | Tooth Extraction | Original Article

Effect of advanced platelet-rich fibrin on accelerating alveolar bone formation in dogs: a histological and immunofluorescence evaluation

Authors: Masahiro To, Chen-yao Su, Kouki Hidaka, Toshimitsu Okudera, Masato Matsuo

Published in: Anatomical Science International | Issue 3/2019

Abstract

Several methods have been developed to regenerate lost alveolar bone. Platelet-rich fibrin (PRF) is a useful adjunct for new bone formation in dentistry. To elucidate the effect of advanced PRF (A-PRF) on bone formation, we inserted A-PRF clots in sockets after tooth extraction. Premolars were extracted from beagle dogs, and A-PRF was applied to the socket. New bone formation was assessed using histological and immunofluorescence examinations, and the bone formation ratio was evaluated 14 and 30 days postoperatively. Histological examination revealed newly formed bone filling the sockets up to the center in the A-PRF group at 14 days postoperatively, while thick and regular bone trabeculae were arranged in porous bone after 30 days. Higher expressions of osteocalcin and osteopontin were observed in newly formed bone in the A-PRF group, compared to the control group. The bone formation ratio was also higher in the A-PRF group than in the control group. Thus, A-PRF application may result in enhanced new bone formation and may aid in accelerating bone formation. A-PRF was more rapid than a self-limiting process during induction of bone formation by enhancing osteoblast activity and may be useful for bone formation in clinical medicine.

Anitua E (1999) Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 14:529–535PubMed

Bowler D, Dym H (2015) Bone morphogenic protein: application in implant dentistry. Dent Clin N Am 59:493–503CrossRefPubMed

Caridade SG, Mano JF (2017) Engineering membranes for bone regeneration. Tissue Eng Part A 23:1502–1533CrossRefPubMed

Choukroun J, Diss A, Simonpieri A et al (2006a) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e56–60CrossRefPubMed

Choukroun J, Diss A, Simonpieri A et al (2006b) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:299–303CrossRefPubMed

Choukroun JI, Braccini F, Diss A, Giordano G, Doglioli P, Dohan DM (2007) Influence of platelet rich fibrin (PRF) on proliferation of human preadipocytes and tympanic keratinocytes: a new opportunity in facial lipostructure (Coleman's technique) and tympanoplasty? Rev Laryngol Otol Rhinol (Bord) 128:27–32

Clark D, Rajendran Y, Paydar S et al (2018) Advanced platelet-rich fibrin and freeze-dried bone allograft for ridge preservation: a randomized controlled clinical trial. J Periodontol 89:379–387CrossRefPubMedPubMedCentral

Dohan DM, Choukroun J, Diss A et al (2006a) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e37–44CrossRefPubMed

Dohan DM, Choukroun J, Diss A et al (2006b) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: Platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e45–50CrossRefPubMed

Dohan DM, Choukroun J, Diss A et al (2006c) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: Leucocyte activation: a new feature for platelet concentrates? Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e51–55CrossRefPubMed

Dohle E, El Bagdadi K, Sader R, Choukroun J, James Kirkpatrick C, Ghanaati S (2018) Platelet-rich fibrin-based matrices to improve angiogenesis in an in vitro co-culture model for bone tissue engineering. J Tissue Eng Regen Med 12:598–610CrossRefPubMed

Faccio R, Grano M, Colucci S, Zallone AZ, Quaranta V, Pelletier AJ (1998) Activation of alphav beta3 integrin on human osteoclast-like cells stimulates adhesion and migration in response to osteopontin. Biochem Biophys Res Commun 249:522–525CrossRefPubMed

Fujioka-Kobayashi M, Miron RJ, Hernandez M, Kandalam U, Zhang Y, Choukroun J (2017) Optimized platelet-rich fibrin with the low-speed concept: growth factor release, biocompatibility, and cellular response. J Periodontol 88:112–121CrossRefPubMed

Ge J, Yang C, Wang Y, Zheng J, Hua H, Zhu J (2018) Comparison of different grafting materials for treatment of bone defect distal to the molar in canine. Clin Implant Dent Relat Res 20:444–454CrossRefPubMed

Ghanaati S, Booms P, Orlowska A et al (2014) Advanced platelet-rich fibrin: a new concept for cell-based tissue engineering by means of inflammatory cells. J Oral Implantol 40:679–689CrossRef

Giachelli CM, Scatena M, Wada T (1997) Osteopontin: potential roles in vascular function and dystrophic calcification. J Bone Miner Metab 15:179–183CrossRef

Hakki SS, Korkusuz P, Purali N et al (2013) Periodontal ligament cell behavior on different titanium surfaces. Acta Odontol Scand 71:906–916CrossRefPubMed

Heinegard D, Andersson G, Reinholt FP (1995) Roles of osteopontin in bone remodeling. Ann N Y Acad Sci 760:213–222CrossRefPubMed

Horimizu M, Kubota T, Kawase T et al (2017) Synergistic effects of the combined use of human-cultured periosteal sheets and platelet-rich fibrin on bone regeneration: an animal study. Clin Exp Dent Res 3:134–141CrossRefPubMedPubMedCentral

Iwasaki K, Munemasa N, Tago K et al (2013) The influence of beta-tricalcium phosphate blocks containing extracellular matrix on osteogenic differentiation of rat bone marrow stromal cells. J Periodontol 84:1484–1492CrossRefPubMed

Kamal KC, Alexandru DO, Rogoveanu OC et al (2018) Immunohistochemical analysis of bone metabolism in osteonecrosis of the femoral head. Rom J Morphol Embryol 59:819–824PubMed

Kobayashi E, Fluckiger L, Fujioka-Kobayashi M et al (2016) Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig 20:2353–2360CrossRefPubMed

Marrelli M, Tatullo M (2013) Influence of PRF in the healing of bone and gingival tissues. Clinical and histological evaluations. Eur Rev Med Pharmacol Sci 17:1958–1962PubMed

Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:638–646CrossRefPubMed

Masuki H, Okudera T, Watanebe T et al (2016) Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent 2:19CrossRefPubMedPubMedCentral

Matsuo M, Okudera T, Iwamiya M (2011) Regeneration of microcirculation and alveolar bone after application of platelet-rich plasma. Microvasc Rev Commun 4:12–17CrossRef

Mihaylova Z, Mitev V, Stanimirov P, Isaeva A, Gateva N, Ishkitiev N (2017) Use of platelet concentrates in oral and maxillofacial surgery: an overview. Acta Odontol Scand 75:1–11CrossRefPubMed

Miron RJ, Zhang YF (2012) Osteoinduction: a review of old concepts with new standards. J Dent Res 91:736–744CrossRefPubMed

Takita H, Tsuruga E, Ono I, Kuboki Y (1997) Enhancement by bFGF of osteogenesis induced by rhBMP-2 in rats. Eur J Oral Sci 105:588–592CrossRefPubMed

Torres J, Tamimi F, Martinez PP et al (2009) Effect of platelet-rich plasma on sinus lifting: a randomized-controlled clinical trial. J Clin Periodontol 36:677–687CrossRefPubMed

Yang D, Liu R, Liu L, Liao H, Wang C, Cao Z (2017) Involvement of CD147 in alveolar bone remodeling and soft tissue degradation in experimental periodontitis. J Periodontal Res 52:704–712CrossRefPubMed

Yoon JS, Lee SH, Yoon HJ (2014) The influence of platelet-rich fibrin on angiogenesis in guided bone regeneration using xenogenic bone substitutes: a study of rabbit cranial defects. J Craniomaxillofac Surg 42:1071–1077CrossRefPubMed

Title: Effect of advanced platelet-rich fibrin on accelerating alveolar bone formation in dogs: a histological and immunofluorescence evaluation
Authors: Masahiro To
Chen-yao Su
Kouki Hidaka
Toshimitsu Okudera
Masato Matsuo
Publication date: 01-06-2019
Publisher: Springer Singapore
Keywords: Tooth Extraction
Tooth Extraction
Published in: Anatomical Science International / Issue 3/2019
Print ISSN: 1447-6959
Electronic ISSN: 1447-073X
DOI: https://doi.org/10.1007/s12565-019-00479-1

At a glance: The STEP trials

Springer Medicine

Effect of advanced platelet-rich fibrin on accelerating alveolar bone formation in dogs: a histological and immunofluorescence evaluation

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2019

Comparative morphology of the lingual papillae and their connective tissue cores in the tongue of the Abyssinian black-and-white colobus (Colobus guereza)

A rare variation of the hemiazygos vein draining into the persistent left superior vena cava

Correction to: Untreated incomplete isolated cleft palate: cadaveric findings

Can feline (Felis catus) flat and long bone morphometry predict sex or skull shape?

Cone-beam computed tomographic evaluation of accessory mental foramen in a Turkish population

Nerve to mylohyoid branched from the lingual nerve: previously undescribed case