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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2010

Open Access 01-12-2010 | Research

Tissue-specific human beta-defensins (HBD)1, HBD2, and HBD3 secretion from human extra-placental membranes stimulated with Escherichia coli

Authors: Guadalupe Garcia-Lopez, Pilar Flores-Espinosa, Veronica Zaga-Clavellina

Published in: Reproductive Biology and Endocrinology | Issue 1/2010

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Abstract

Background

During an ascending infection along the reproductive tract, the extra-placental membranes must act as a selective and competent barrier against pathogens. Human beta defensins (HBD)1, HBD2, and HBD3 are key elements of innate immunity that are secreted to neutralize/control the progression of infection.

Methods

Full-thickness membranes were mounted on a Transwell device, constituted by two independent chambers, 1 × 10(6) CFU/ml of Escherichia coli were added to either the amnion (AMN) or the choriodecidual (CHD) face or to both. Secretion profiles of HBD1, HBD2, and HBD3 to the culture medium were quantified by ELISA.

Results

In comparison with basal conditions, the secretion profile of HBD1 remained without significant changes; HBD2 level in CHD and AMN increased 1.9- and 1.4-times, respectively, after stimulation with bacteria. HBD3 secretion level increased significantly (7.8 +/- 1.9 pg/micrograms) in the CHD but only if the stimulus was applied on the AMN side.

Conclusions

Selective stimulation of extra-placental membranes with E. coli, results in a tissue specific secretion of HBD1, HBD2, and HBD3 mainly in the CHD, which is the first infected region during an ascending infection.
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Literature
1.
2.
Asrat T: Intra-amniotic infection in patients with preterm prelabor rupture of membranes. Pathophysiology, detection, and management. Clin Perinatol. 2001, 28 (4): 735-751. 10.1016/S0095-5108(03)00074-5.CrossRefPubMed
3.
Romero R, Mazor M: Infection and preterm labor. Clin Obstet Gynecol. 1988, 31 (3): 553-584. 10.1097/00003081-198809000-00006.CrossRefPubMed
4.
5.
Yang D, Chertov O, Bykovskaia SN, Chen Q, Buffo MJ, Shogan J, Anderson M, Schroder JM, Wang JM, Howard OM: Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science. 1999, 286 (5439): 525-528. 10.1126/science.286.5439.525.CrossRefPubMed
6.
King AE, Kelly RW, Sallenave JM, Bocking AD, Challis JR: Innate immune defences in the human uterus during pregnancy. Placenta. 2007, 28 (11-12): 1099-1106. 10.1016/j.placenta.2007.06.002.CrossRefPubMed
7.
King AE, Paltoo A, Kelly RW, Sallenave JM, Bocking AD, Challis JR: Expression of natural antimicrobials by human placenta and fetal membranes. Placenta. 2007, 28 (2-3): 161-169. 10.1016/j.placenta.2006.01.006.CrossRefPubMed
8.
Soto E, Espinoza J, Nien JK, Kusanovic JP, Erez O, Richani K, Santolaya-Forgas J, Romero R: Human beta-defensin-2: a natural antimicrobial peptide present in amniotic fluid participates in the host response to microbial invasion of the amniotic cavity. J Matern Fetal Neonatal Med. 2007, 20 (1): 15-22. 10.1080/14767050601036212.PubMedCentralCrossRefPubMed
9.
Moyo SR, Tswana SA, Nystrom L, Bergstrom S, Blomberg J, Ljungh A: Intrauterine death and infections during pregnancy. Int J Gynaecol Obstet. 1995, 51 (3): 211-218. 10.1016/0020-7292(95)80004-2.CrossRefPubMed
10.
Deb K, Chaturvedi MM, Jaiswal YK: Comprehending the role of LPS in Gram-negative bacterial vaginosis: ogling into the causes of unfulfilled child-wish. Arch Gynecol Obstet. 2004, 270 (3): 133-146. 10.1007/s00404-004-0623-0.CrossRefPubMed
11.
Duncan JR, Cock ML, Scheerlinck JP, Westcott KT, McLean C, Harding R, Rees SM: White matter injury after repeated endotoxin exposure in the preterm ovine fetus. Pediatr Res. 2002, 52 (6): 941-949.CrossRefPubMed
12.
Davies JK, Shikes RH, Sze CI, Leslie KK, McDuffie RS, Romero R, Gibbs RS: Histologic inflammation in the maternal and fetal compartments in a rabbit model of acute intra-amniotic infection. Am J Obstet Gynecol. 2000, 183 (5): 1088-1093. 10.1067/mob.2000.108888.CrossRefPubMed
13.
Zaga V, Estrada-Gutierrez G, Beltran-Montoya J, Maida-Claros R, Lopez-Vancell R, Vadillo-Ortega F: Secretions of interleukin-1beta and tumor necrosis factor alpha by whole fetal membranes depend on initial interactions of amnion or choriodecidua with lipopolysaccharides or group B streptococci. Biol Reprod. 2004, 71 (4): 1296-1302. 10.1095/biolreprod.104.028621.CrossRefPubMed
14.
Zaga-Clavellina V, Garcia-Lopez G, Flores-Herrera H, Espejel-Nunez A, Flores-Pliego A, Soriano-Becerril D, Maida-Claros R, Merchant-Larios H, Vadillo-Ortega F: In vitro secretion profiles of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and TNF alpha after selective infection with Escherichia coli in human fetal membranes. Reprod Biol Endocrinol. 2007, 5: 46-10.1186/1477-7827-5-46.PubMedCentralCrossRefPubMed
15.
Thiex NW, Chames MC, Loch-Caruso RK: Tissue-specific cytokine release from human extra-placental membranes stimulated by lipopolysaccharide in a two-compartment tissue culture system. Reprod Biol Endocrinol. 2009, 7: 117-10.1186/1477-7827-7-117.PubMedCentralCrossRefPubMed
16.
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-254. 10.1016/0003-2697(76)90527-3.CrossRefPubMed
17.
Krisanaprakornkit S, Weinberg A, Perez CN, Dale BA: Expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue. Infect Immun. 1998, 66 (9): 4222-4228.PubMedCentralPubMed
18.
Sorensen OE, Thapa DR, Rosenthal A, Liu L, Roberts AA, Ganz T: Differential regulation of beta-defensin expression in human skin by microbial stimuli. J Immunol. 2005, 174 (8): 4870-4879.CrossRefPubMed
19.
Duits LA, Ravensbergen B, Rademaker M, Hiemstra PS, Nibbering PH: Expression of beta-defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells. Immunology. 2002, 106 (4): 517-525. 10.1046/j.1365-2567.2002.01430.x.PubMedCentralCrossRefPubMed
20.
Krisanaprakornkit S, Kimball JR, Weinberg A, Darveau RP, Bainbridge BW, Dale BA: Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier. Infect Immun. 2000, 68 (5): 2907-2915. 10.1128/IAI.68.5.2907-2915.2000.PubMedCentralCrossRefPubMed
21.
O'Neil DA, Porter EM, Elewaut D, Anderson GM, Eckmann L, Ganz T, Kagnoff MF: Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J Immunol. 1999, 163 (12): 6718-6724.PubMed
22.
Ogushi K, Wada A, Niidome T, Mori N, Oishi K, Nagatake T, Takahashi A, Asakura H, Makino S, Hojo H: Salmonella enteritidis FliC (flagella filament protein) induces human beta-defensin-2 mRNA production by Caco-2 cells. J Biol Chem. 2001, 276 (32): 30521-30526. 10.1074/jbc.M011618200.CrossRefPubMed
23.
Vora P, Youdim A, Thomas LS, Fukata M, Tesfay SY, Lukasek K, Michelsen KS, Wada A, Hirayama T, Arditi M: Beta-defensin-2 expression is regulated by TLR signaling in intestinal epithelial cells. J Immunol. 2004, 173 (9): 5398-5405.CrossRefPubMed
24.
25.
Stock SJ, Kelly RW, Riley SC, Calder AA: Natural antimicrobial production by the amnion. Am J Obstet Gynecol. 2007, 196 (3): 255-10.1016/j.ajog.2006.10.908. e251-256PubMed
26.
Reznikov LL, Fantuzzi G, Selzman CH, Shames BD, Barton HA, Bell H, McGregor JA, Dinarello CA: Utilization of endoscopic inoculation in a mouse model of intrauterine infection-induced preterm birth: role of interleukin 1beta. Biol Reprod. 1999, 60 (5): 1231-1238. 10.1095/biolreprod60.5.1231.CrossRefPubMed
27.
Kent AS, Sullivan MH, Elder MG: Transfer of cytokines through human fetal membranes. J Reprod Fertil. 1994, 100 (1): 81-84. 10.1530/jrf.0.1000081.CrossRefPubMed
28.
Menon R, Swan KF, Lyden TW, Rote NS, Fortunato SJ: Expression of inflammatory cytokines (interleukin-1 beta and interleukin-6) in amniochorionic membranes. Am J Obstet Gynecol. 1995, 172 (2 Pt 1): 493-500. 10.1016/0002-9378(95)90562-6.CrossRefPubMed
29.
Utech M, Bruwer M, Nusrat A: Tight junctions and cell-cell interactions. Methods Mol Biol. 2006, 341: 185-195.PubMed
30.
Harder J, Bartels J, Christophers E, Schroder JM: Isolation and characterization of human beta -defensin-3, a novel human inducible peptide antibiotic. J Biol Chem. 2001, 276 (8): 5707-5713. 10.1074/jbc.M008557200.CrossRefPubMed
31.
Duits LA, Nibbering PH, van Strijen E, Vos JB, Mannesse-Lazeroms SP, van Sterkenburg MA, Hiemstra PS: Rhinovirus increases human beta-defensin-2 and -3 mRNA expression in cultured bronchial epithelial cells. FEMS Immunol Med Microbiol. 2003, 38 (1): 59-64. 10.1016/S0928-8244(03)00106-8.CrossRefPubMed
32.
Buhimschi IA, Jabr M, Buhimschi CS, Petkova AP, Weiner CP, Saed GM: The novel antimicrobial peptide beta3-defensin is produced by the amnion: a possible role of the fetal membranes in innate immunity of the amniotic cavity. Am J Obstet Gynecol. 2004, 191 (5): 1678-1687. 10.1016/j.ajog.2004.03.081.CrossRefPubMed
33.
Hirsch E, Saotome I, Hirsh D: A model of intrauterine infection and preterm delivery in mice. Am J Obstet Gynecol. 1995, 172 (5): 1598-1603. 10.1016/0002-9378(95)90503-0.CrossRefPubMed
34.
Vadillo-Ortega F, Sadowsky DW, Haluska GJ, Hernandez-Guerrero C, Guevara-Silva R, Gravett MG, Novy MJ: Identification of matrix metalloproteinase-9 in amniotic fluid and amniochorion in spontaneous labor and after experimental intrauterine infection or interleukin-1 beta infusion in pregnant rhesus monkeys. Am J Obstet Gynecol. 2002, 186 (1): 128-138. 10.1067/mob.2002.118916.CrossRefPubMed
35.
Koga K, Aldo PB, Mor G: Toll-like receptors and pregnancy: trophoblast as modulators of the immune response. J Obstet Gynaecol Res. 2009, 35 (2): 191-202. 10.1111/j.1447-0756.2008.00963.x.CrossRefPubMed
36.
Kim YM, Romero R, Chaiworapongsa T, Kim GJ, Kim MR, Kuivaniemi H, Tromp G, Espinoza J, Bujold E, Abrahams VM: Toll-like receptor-2 and -4 in the chorioamniotic membranes in spontaneous labor at term and in preterm parturition that are associated with chorioamnionitis. Am J Obstet Gynecol. 2004, 191 (4): 1346-1355. 10.1016/j.ajog.2004.07.009.CrossRefPubMed
37.
Hayati AR, Mohamed AE, Tan GC: An immunohistochemical study of Toll-like receptors 2 and 4 in placenta with and without infection. Malays J Pathol. 32 (1): 13-19.
Metadata
Title
Tissue-specific human beta-defensins (HBD)1, HBD2, and HBD3 secretion from human extra-placental membranes stimulated with Escherichia coli
Authors
Guadalupe Garcia-Lopez
Pilar Flores-Espinosa
Veronica Zaga-Clavellina
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2010
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-8-146

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