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Journal of Hepato-Biliary-Pancreatic Sciences

Published in:

01-07-2009 | Original article

Tissue factor expression demonstrates severe sinusoidal endothelial cell damage during rejection after living-donor liver transplantation

Authors: Masanobu Usui, Naohisa Kuriyama, Masashi Kisawada, Takashi Hamada, Shugo Mizuno, Hiroyuki Sakurai, Masami Tabata, Hiroshi Imai, Kohji Okamoto, Shinji Uemoto, Shuji Isaji

Published in: Journal of Hepato-Biliary-Pancreatic Sciences | Issue 4/2009

Abstract

Background/Purpose

Since it is well known that endothelial cells may be important targets during rejection after living-donor liver transplantation, in this study we investigated liver sinusoidal endothelial cell (SEC) damage during rejection by focusing on thrombomodulin (TM) and hyaluronic acid (HA) as plasma markers of SEC damage. We also examined tissue factor (TF) expression in SECs, because damage to endothelial cells leads to immediate activation of the coagulation system, with the damage being triggered mainly by TF.

Methods

Living-donor liver transplantation was performed at Mie University Hospital between March 2002 and December 2007; there were 8 patients with rejection (4 with acute cellular rejection and 4 with chronic rejection) and 32 patients without rejection. Liver biopsy tissue was immunostained with an anti-TF antibody, and assessed for SEC damage. In addition, total RNA was extracted from liver biopsy tissue and tested for TF mRNA expression by reverse-transcription polymerase chain reaction (RT-PCR).

Results

The plasma TM level was significantly higher in the rejection group than in the non-rejection group. TF expression was observed in SECs, in infiltrating inflammatory cells, and in the vascular endothelium in the rejection group. TF mRNA expression was significantly higher in the rejection group than in the non-rejection group.

Conclusions

We demonstrated that TF expression revealed severe SEC damage in grafted liver during both acute cellular rejection and chronic rejection.

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Title: Tissue factor expression demonstrates severe sinusoidal endothelial cell damage during rejection after living-donor liver transplantation
Authors: Masanobu Usui
Naohisa Kuriyama
Masashi Kisawada
Takashi Hamada
Shugo Mizuno
Hiroyuki Sakurai
Masami Tabata
Hiroshi Imai
Kohji Okamoto
Shinji Uemoto
Shuji Isaji
Publication date: 01-07-2009
Publisher: Springer Japan
Published in: Journal of Hepato-Biliary-Pancreatic Sciences / Issue 4/2009
Print ISSN: 1868-6974
Electronic ISSN: 1868-6982
DOI: https://doi.org/10.1007/s00534-009-0095-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Tissue factor expression demonstrates severe sinusoidal endothelial cell damage during rejection after living-donor liver transplantation

Abstract

Background/Purpose

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background/Purpose

Methods

Results

Conclusions

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