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01-12-2020 | Thrombosis | Research

Antithrombotic effect and action mechanism of Salvia miltiorrhiza and Panax notoginseng herbal pair on the zebrafish

Authors: Shi-Jun Yin, Ying-Qing Luo, Cong-Peng Zhao, Hua Chen, Zhang-Feng Zhong, Shengpeng Wang, Yi-Tao Wang, Feng-Qing Yang

Published in: Chinese Medicine | Issue 1/2020

Abstract

Background

Salvia miltiorrhiza (Danshen, DS) and Panax notoginseng (Sanqi, SQ) are famous traditional Chinese herbs, and their herbal pair (DS–SQ) has been popular used as anti-thrombotic medicines. However, there is still a lack of sufficient scientific evidence to illustrate the optimum combination ratio of these two herbs as well as its action mechanisms. The purpose of this study is to investigate the anti-thrombotic effects of DS–SQ on zebrafish and explore its possible action mechanism.

Methods

Firstly, the chemical components in DS–SQ extract were analyzed by LC–ESI–MS/MS. Then, a phenylhydrazine (PHZ)-induced zebrafish thrombosis model was developed for evaluating the anti-thrombotic effects of DS–SQ extracts with different combination ratios and their nine pure compounds. Followed, Real-time quantitative PCR (RT-qPCR) assays were performed to investigate the potential antithrombotic mechanisms of DS–SQ.

Results

Thirty-three components were tentatively identified by LC–MS analysis. DS–SQ at the ratio of 10:1 presented the best anti-thrombotic effect, and rosmarinic acid, lithospermic acid and salvianolic acid B of DS showed good anti-thrombotic activity on zebrafish thrombosis model. The RT-qPCR assays indicated that DS–SQ (10:1) could cure the PHZ-induced thrombosis by downregulating the expression of PKCα, PKCβ, fga, fgb, fgg and vWF in zebrafish.

Conclusions

DS–SQ with the combination ratio of 10:1 showed optimum anti-thrombotic effect on PHZ-induced zebrafish thrombosis model, which provided a reference for reasonable clinical applications of DS–SQ herbal pair.

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Title: Antithrombotic effect and action mechanism of Salvia miltiorrhiza and Panax notoginseng herbal pair on the zebrafish
Authors: Shi-Jun Yin
Ying-Qing Luo
Cong-Peng Zhao
Hua Chen
Zhang-Feng Zhong
Shengpeng Wang
Yi-Tao Wang
Feng-Qing Yang
Publication date: 01-12-2020
Publisher: BioMed Central
Keyword: Thrombosis
Published in: Chinese Medicine / Issue 1/2020
Electronic ISSN: 1749-8546
DOI: https://doi.org/10.1186/s13020-020-00316-y

At a glance: The STEP trials

Springer Medicine

Antithrombotic effect and action mechanism of Salvia miltiorrhiza and Panax notoginseng herbal pair on the zebrafish

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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