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Inflammation Research
Published in: Inflammation Research 11/2013

01-11-2013 | Original Research Paper

Therapeutic potential of biodegradable microparticles containing Punica granatum L. (pomegranate) in murine model of asthma

Authors: Jéssica F. F. de Oliveira, Diego V. Garreto, Mayara C. P. da Silva, Thiare S. Fortes, Rejane B. de Oliveira, Flávia R. F. Nascimento, Fernando B. Da Costa, Marcos A. G. Grisotto, Roberto Nicolete

Published in: Inflammation Research | Issue 11/2013

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Abstract

Objective and design

Among the options for treatment of diseases affecting the respiratory system, especially asthma, drug delivering systems for intranasal application represent an important therapeutic approach at the site of inflammation. The present study aimed to evaluate the therapeutic effect of biodegradable microparticles formed by poly lactic-co-glycolic acid (PLGA) containing encapsulated pomegranate extract on a murine model of asthma.

Material

The extract was acquired from the leaves of P. granatum and characterized qualitatively by HPLC. A w/o/w emulsion solvent extraction–evaporation method was chosen to prepare the microparticles containing pomegranate encapsulated extract (MP).

Treatment

OVA-sensitized BALB/c mice were used as asthma model and treated with dexamethasone and P. granatum extract in solution form or encapsulated into microparticles.

Results

MP were able to inhibit leukocytes’ recruitment to bronchoalveolar fluid, especially, eosinophils, decreasing cytokines (IL-1β and IL-5) and protein levels in the lungs.

Conclusions

This approach can be used as an alternative/supplementary therapy based on the biological effects of P. granatum for managing inflammatory processes, especially those with pulmonary complications.
Literature
1.
Barnes PJ. Pathophysiology of asthma. Eur Respir Mon. 2003;8:84–113.
2.
3.
4.
Yang EJ, Lee JS, Yun CY, Kim JH, Kim JS, Kim DH, Kim IS. Inhibitory effects of Duchesnea chrysantha extract on ovalbumin-induced lung inflammation in a mouse model of asthma. J Ethnopharmacol. 2008;118:102–7.PubMedCrossRef
5.
Yuk JE, Woo JS, Yun CY, Lee JS, Kim JH, Song GY, Yang EJ, Hur IK, Kim IS. Effects of lactose-β-sitosterol and β-sitosterol on ovalbumin-induced lung inflammation in actively sensitized mice. Int Immunopharmacol. 2007;7:1517–27.PubMedCrossRef
6.
7.
Verpoorte R. Exploration of nature’s chemodiversity: the role of secondary metabolites as leads in drug development. Drug Discov Today. 1999;3:232–8.CrossRef
8.
Koehn FE, Carter GT. The evolving role of natural products in drug discovery. Nat Rev Drug Discov. 2005;4:206–20.PubMedCrossRef
9.
Rogerio AP, Fontanari C, Borducchi E, Keller AC, Russo M, Soares EG, Albuquerque DA, Faccioli LH. Anti-inflammatory effects of Lafoensia pacari and ellagic acid in a murine model of asthma. Eur J Pharmacol. 2008;580:262–70.PubMedCrossRef
10.
Rogerio AP. SÁ-NUNES A, Faccioli LH. The activity of medicinal plants and secondary metabolites on eosinophilic inflammation. Pharmacol Res. 2010;62:298–307.PubMedCrossRef
11.
Jurenka JS. Therapeutic applications of pomegranate (Punica granatum L.): a review. Altern Med Rev. 2008;13:128–44.PubMed
12.
Fischer UA, Carle R, Kammerer DR. Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD–ESI/MSn. Food Chem. 2011;127:807–21.PubMedCrossRef
13.
Lansky EP, Newman RA. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J Ethnopharmacol. 2007;109:177–206.PubMedCrossRef
14.
Lansky E, Shubert S, Newman I. Pharmacological and therapeutic properties of pomegranate. CIHEAM–Options Mediterraneennes. 2004; 231–235.
15.
Lee SI, Kim BS, Kim KS, Lee S, Shin KS, Lim JS. Immune-suppressive activity of punicalagin via inhibition of NFAT activation. Biochem Biophys Res Commun. 2008;371:799–803.PubMedCrossRef
16.
Romier B, Van De Walle J, During A, Larondelle Y, Schneider YJ. Modulation of signalling nuclear factor-kappaB activation pathway by polyphenols in human intestinal Caco-2 cells. Br J Nutr. 2008;100:542–51.PubMedCrossRef
17.
Nicolete R, Lima KM, Rodrigues Júnior JM, Baruffi MD, Medeiros AI, Bentley MVLB, Silva CL, Faccioli LH. In vitro and in vivo activities of leukotrienes B4-loaded biodegradable microspheres. Prostaglandins Other Lipid Mediators. 2007;83:121–9.PubMedCrossRef
18.
Nicolete R, Rius C, Piqueras L, Jose PJ, Sorgi CA, Soares EG, Sanz MJ, Faccioli LH. Leukotriene B4-loaded microspheres: a new therapeutic strategy to modulate cell activation. BMC Immunol. 2008;9:1–11.CrossRef
19.
Oh YJ, Lee J, Seo JY, Rhim T, Kim S, Yoon HJ, Lee KY. Preparation of budesonide-loaded porous PLGA microparticles and their therapeutic efficacy in a murine asthma model. J Controlled Release. 2011;150:56–62.CrossRef
20.
Sung JC, Pulliam BL, Edwards DA. Nanoparticles for drug delivery to the lungs. Trends Biotechnol. 2007;25:563–70.PubMedCrossRef
21.
Pilcer G, Amighi K. Formulation strategy and use of excipients in pulmonary drug delivery. Int J Pharm. 2010;392:1–19.PubMedCrossRef
22.
Bekir J, Mars M, Souchard JP, Bouajila J. Assessment of antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of pomegranate (Punica granatum) leaves. Food Chem Toxicol. 2013;55:470–5.PubMedCrossRef
23.
Gil MI, Tomás-Barberán FA, Hess-Pierce B, Holcroft DM, Kader AA. Antioxidant Activity of Pomegranate Juice and Its Relationship with Phenolic Composition and Processing. J Agric Food Chem. 2000;48:4581–9.PubMedCrossRef
24.
Ismail T, Sestili P, Akhtar S. Pomegranate peel and fruit extracts: a review of potential anti-inflammatory and anti-infective effects. J Ethnopharmacol. 2012;143:397–405.PubMedCrossRef
25.
Wager S, Lundblad LKA, Ekman M, Irvin CG, Bates JHT. The allergic mouse model of asthma: normal smooth muscle in an abnormal lung? J Appl Physiol. 2004;96:2019–27.CrossRef
26.
Nicolete R, Secatto A, Pereira PAT, Soares EG, Faccioli LH. Leukotriene B4-loaded microspheres as a new approach to enhance antimicrobial responses in Histoplasma capsulatum-infected mice. Int J Antimicr Agets. 2009;4:365–9.CrossRef
27.
Cook-Mills JM, Deem TL. Active participation of endothelial cells in inflammation. J Leukoc Biol. 2005;77:487–95.PubMedCrossRef
28.
Balwani S, Nandi D, Jaisankar P, Ghosh B. 2-Methyl-pyran-4-one-3-O-β-D-glucopyranoside isolated from leaves of Punica granatum inhibits the TNFα-induced cell adhesion molecules expression by blocking nuclear transcription factor-кB (NF-кB). Biochimie. 2011;93:921–30.PubMedCrossRef
29.
Nicolete R, Santos DF, Faccioli LH. The uptake of PLGA micro or nanoparticles by macrophages provokes distinct in vitro inflammatory response. Int Immunopharmacol. 2011;11:1557–63.PubMedCrossRef
30.
Whelan R, Kim C, Chen M, Leiter J, Grunstein MM, Hakonarson H. Role and regulation of interleukin-1 molecules in pro-asthmatic sensitised airway smooth muscle. Eur Respir J. 2004;24:559–67.PubMedCrossRef
31.
Faccioli LH, Mokwa VF, Silva CL, Rocha GM, Araujo JI, Nahori MA, Vargaftig BB. IL-5 drives eosinophils from bone marrow to blood and tissues in a guinea-pig model of visceral larva migrans syndrome. Mediators Inflamm. 1996;5:24–31.PubMedCrossRef
32.
Rogerio AP, Sá-Nunes A, Albuquerque DA, Anibal FF, Medeiros AI, Machado ER, Souza AO, Prado JC Jr, Faccioli LH. Lafoensia pacari extract inhibits IL-5 production in toxocariasis. Parasite Immunol. 2003;25:393–400.PubMedCrossRef
33.
Lampinen M, Carlson M, Hakansson LD, Venge P. Cytokine-regulated accumulation of eosinophils in inflammatory disease. Allergy. 2004;59:793–805.PubMedCrossRef
Metadata
Title
Therapeutic potential of biodegradable microparticles containing Punica granatum L. (pomegranate) in murine model of asthma
Authors
Jéssica F. F. de Oliveira
Diego V. Garreto
Mayara C. P. da Silva
Thiare S. Fortes
Rejane B. de Oliveira
Flávia R. F. Nascimento
Fernando B. Da Costa
Marcos A. G. Grisotto
Roberto Nicolete
Publication date
01-11-2013
Publisher
Springer Basel
Published in
Inflammation Research / Issue 11/2013
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-013-0659-3

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