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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2012

Open Access 01-12-2012 | Research article

The roots of Atractylodes japonica Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells

Authors: Yunkyung Han, Hyo Won Jung, Yong-Ki Park

Published in: BMC Complementary Medicine and Therapies | Issue 1/2012

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Abstract

Background

Type 2 diabetes (T2D) is a complex metabolic disorder characterized by insulin resistance and hyperglycemia. Peroxisome proliferator-activated receptor gamma (PPARγ) is a key transcription factor and plays an important role in the regulation of genes involved in adipogenic differentiation, glucose metabolism and insulin signal transduction.

Methods

In this study, the effects of the root extract of Atractylodes japonica Koidzumi ( Atractylodis Rhizoma Alba, ARA) on the differentiation of 3T3-L1 preadipocytes and the possible mechanism of glucose transport were investigated. 3T3-L1 cells were cultured with insulin and ARA extract.

Results

In 3T3-L1 cells, ARA extract significantly enhanced adipogenic differentiation and upregulated the expression of PPARγ genes and protein in a dose-dependent manner. ARA also promoted glucose transport by increasing the glucose transporter 4 (GLUT-4), phosphatidylinositol 3-kinase (PI3K) and insulin receptor substrates-1 (IRS-1) levels.

Conclusion

Our results suggest that ARA extract may be an attractive therapeutic agent for managing T2D via promoting the differentiation of adipocytes with the upregulation of PPARγ levels and the activation of the insulin signaling pathway.
Appendix
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Literature
1.
2.
Zhou J, Zhou S: Berberine regulates peroxisome proliferator-activated receptors and positive transcription elongation factor b expression in diabetic adipocytes. Eur J Pharmacol. 2010, 649: 390-397. 10.1016/j.ejphar.2010.09.030.CrossRefPubMed
3.
Castan-Laurell I, Dray C, Knauf C, Kunduzova O, Valet P: Apelin, a promising target for type 2 diabetes treatment?. Trends Endocrinol Metab. 2012, 23: 234-241. 10.1016/j.tem.2012.02.005.CrossRefPubMed
4.
Burén J, Liu HX, Lauritz J, Eriksson JW: High glucose and insulin in combination cause insulin receptor substrate-1 and −2 depletion and protein kinase B desensitization in primary cultured rat adipocytes: possible implication for insulin resistance in type 2 diabetes. Eur J Endocrinol. 2003, 148: 157-167. 10.1530/eje.0.1480157.CrossRefPubMed
5.
Xue P, Hou Y, Zhang Q, Woods CG, Yarborough K, Liu H, Sun G, Anderson ME, Pi J: Prolonged inorganic arsenite exposure suppresses insulin-stimulated AKT S473 phosphorylation and glucose uptake in 3T3-L1 adipocytes: involvement of the adaptive antioxidant response. Biochem Biophys Res Commun. 2011, 407: 360-365. 10.1016/j.bbrc.2011.03.024.CrossRefPubMedPubMedCentral
6.
Ahn J, Lee H, Kim S, Ha T: Curcumin-induced suppression of adipogenic differentiation is accompanied by activation of Wnt/β-catenin signaling. Am J Physiol Cell Physiol. 2010, 298: 1510-1516. 10.1152/ajpcell.00369.2009.CrossRef
7.
Liu J, Lin H, Cheng P, Hu X, Lu H: Effects of ghrelin on the proliferation and differentiation of 3T3-L1 preadipocytes. J Huazhong Univ Sci Technol. 2009, 29: 227-230. 10.1007/s11596-009-0218-x.CrossRef
8.
Kim CK, Kim M, Oh SD, Lee SM, Sun B, Choi GS, Kim SK, Bae H, Kang C, Min BI: Effects of Atractylodes macrophala Koidzumi rhizome on 3T3-L1 adipogenesis and an animal model of obesity. J Ethnopharmacol. 2011, 137: 396-402. 10.1016/j.jep.2011.05.036.CrossRefPubMed
9.
Zhang WY, Lee JJ, Kim IS, Kim Y, Park JS, Myung CS: 7-O-Methylaromadendrin stimulates glucose uptake and improves insulin resistance in vitro. Biol Pharm Bull. 2010, 33: 1494-1499. 10.1248/bpb.33.1494.CrossRefPubMed
10.
Xu C, Wang LL, Liu HY, Zhou XB, Cao YL, Li S: C33H, a novel PPARα/γ dual agonist, has beneficial effects on insulin resistance and lipid metabolism. Acta Pharmacol Sin. 2006, 27: 223-228. 10.1111/j.1745-7254.2006.00263.x.CrossRefPubMed
11.
Choi K, Kim YB: Molecular mechanism of insulin resistance in obesity and type 2 diabetes. Korean J Intern Med. 2010, 25: 119-129. 10.3904/kjim.2010.25.2.119.CrossRefPubMedPubMedCentral
12.
Lee SH, Lee HJ, Lee YH, Lee BW, Cha BS, Kang ES, Ahn CW, Park JS, Kim HJ, Lee EY: Korean red ginseng (panax ginseng) improves insulin sensitivity in high fat fed Sprague–Dawley rats. Phytother Res. 2012, 26: 142-147. 10.1002/ptr.3610.CrossRefPubMed
13.
Kim D, Park KK, Lee SK, Lee SE, Hwang JK: Cornus kousa f. buerger ex miquel increases glucose uptake through activation of peroxisome proliferator-activated receptor gamma and insulin sensitization. J Ethnopharmacol. 2011, 133: 803-809. 10.1016/j.jep.2010.11.007.CrossRefPubMed
14.
Hong MH, Kim JH, Bae H, Lee NY, Shin YC, Kim SH, Ko SG: Atractylodes japonica koidzumi inhibits the production of proinflammatory cytokines through inhibition of the NF-kappaB/IkappaB signal pathway in HMC-1 human mast cells. Arch Pharm Res. 2010, 33: 843-851. 10.1007/s12272-010-0606-6.CrossRefPubMed
15.
Wang KT, Chen LG, Wu CH, Chang CC, Wang CC: Gastroprotective activity of atractylenolide III from Atractylodes ovate on ethanol-induced gastric ulcer in vitro and in vivo. J Pharm Pharmacol. 2010, 62: 381-388.CrossRefPubMed
16.
Wang KT, Chen LG, Chou DS, Liang WL, Wang CC: Anti-oxidative abilities of essential oils from Atractylodes ovate rhizome. Evid Based Complement Alternat Med. 2011, 10.1093/ecam/neq006.
17.
Lee OH, Lee HH, Kim JH, Lee BY: Effect of ginsenosides Rg3 and Re on glucose transport in mature 3T3-L1 adipocytes. Phytother Res. 2011, 25: 768-773.CrossRefPubMed
18.
Ikarashi N, Tajima M, Suzuki K, Toda T, Ito K, Ochiai W, Sugiyama K: Inhibition of preadipocyte differentiation and lipid accumulation by orengedokuto treatment of 3T3-L1 cultures. Phytother Res. 2012, 26: 91-100. 10.1002/ptr.3493.CrossRefPubMed
19.
Sharma AM, Staels B: Peroxisome proliferator-activated receptor gamma and adipose tissue-understanding obesity-related changes in regulation of lipid and glucose metabolism. J Clin Endocrinol Metab. 2007, 92: 386-395.CrossRefPubMed
20.
Yang Y, Shang W, Zhou L, Jiang B, Jin H, Chen M: Emodin with PPARγ ligand-binding activity promotes adipocyte differentiation and increases glucose uptake in 3T3-L1 cells. Biochem Biophys Res Commun. 2007, 353: 225-230. 10.1016/j.bbrc.2006.11.134.CrossRefPubMed
21.
Cho KW, Lee OH, Banz WJ, Moustaid-Moussa N, Shay NF, Kim YC: Daidzein and the daidzein metabolite, equol, enhance adipocyte differentiation and PPARgamma transcriptional activity. J Nutr Biochem. 2010, 21: 841-847. 10.1016/j.jnutbio.2009.06.012.CrossRefPubMed
22.
Oliver E, McGillicuddy F, Phillips C, Toomey S, Roche HM: The role of inflammation and macrophage accumulation in the development of obesity-induced type 2 diabetes mellitus and the possible therapeutic effects of long-chain n-3 PUFA. Proc Nutr Soc. 2010, 69: 232-243. 10.1017/S0029665110000042.CrossRefPubMed
Metadata
Title
The roots of Atractylodes japonica Koidzumi promote adipogenic differentiation via activation of the insulin signaling pathway in 3T3-L1 cells
Authors
Yunkyung Han
Hyo Won Jung
Yong-Ki Park
Publication date
01-12-2012
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2012
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-12-154

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