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European Archives of Oto-Rhino-Laryngology

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01-02-2018 | Rhinology

The role of YKL40 in the pathogenesis of CRS with nasal polyps

Authors: Yue Ma, Chunquan Zheng, Le Shi

Published in: European Archives of Oto-Rhino-Laryngology | Issue 2/2018

Abstract

Background

Many Chinese patients who experience chronic rhinosinusitis with nasal polyps (CRSwNP) have been shown to exhibit specifically enhanced TH1/TH17 responses and excessive neutrophil accumulation without demonstrating significant eosinophilia. These patients may be subject to different pathologies and therapies compared to Western patients. YKL40 can be produced by neutrophil and is associated with many inflammatory diseases, while its role in the pathogenesis of chronic rhinosinusitis (CRS) has yet to be determined.

Objective

The aim of this study was to investigate the relationship between the expression level and biologic role of YKL40 in CRS.

Methods

YKL40 expression was examined via quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), immunohistochemistry, and Western blot. Human nasal epithelia cells (HNECs) were isolated to detect YKL40 expression in response to specific inflammatory stimulation.

Results

YKL40 expression levels were significantly higher in NP patients compared to the turbinates of CRSsNP/CRSwNP and the control group and can be strongly activated by stimulation with IL-4 in vitro and suppressed by the other pro-inflammatory cytokines; lipopolysaccharide (LPS) and dexamethasone also caused significant decreases in YKL40 expression in HNECs.

Conclusions

YKL40 may play a significant role in Chinese patients with CRSwNP. The molecular mechanisms identified here may aid in the design of new therapeutic strategies for improving the clinical outcomes of Chinese patients.

Van Zele T, Claeys S, Gevaert P, Van Maele G, Holtappels G, Van Cauwenberge P, Bachert C (2006) Differentiation of chronic sinus diseases by measurement of inflammatory mediators. Allergy 61(11):1280–1289. https://doi.org/10.1111/j.1398-9995.2006.01225.x CrossRefPubMed

Tomassen P, Van Zele T, Zhang N, Perez-Novo C, Van Bruaene N, Gevaert P, Bachert C (2011) Pathophysiology of chronic rhinosinusitis. Proc Am Thorac Soc 8(1):115–120. https://doi.org/10.1513/pats.201005-036RN CrossRef

Cao PP, Li HB, Wang BF, Wang SB, You XJ, Cui YH, Wang DY, Desrosiers M, Liu Z (2009) Distinct immunopathologic characteristics of various types of chronic rhinosinusitis in adult Chinese. J Allergy Clin Immunol 124(3):478–484, 484.e471–472. https://doi.org/10.1016/j.jaci.2009.05.017

Wen W, Liu W, Zhang L, Bai J, Fan Y, Xia W, Luo Q, Zheng J, Wang H, Li Z, Xia J, Jiang H, Liu Z, Shi J, Li H, Xu G (2012) Increased neutrophilia in nasal polyps reduces the response to oral corticosteroid therapy. J Allergy Clin Immunol 129(6):1522–1528.e1525. https://doi.org/10.1016/j.jaci.2012.01.079 CrossRefPubMed

Meng G, Zhao Y, Bai X, Liu Y, Green TJ, Luo M, Zheng X (2010) Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity. J Biol Chem 285(51):39898–39904. https://doi.org/10.1074/jbc.M110.130781 CrossRefPubMedPubMedCentral

Kawada M, Hachiya Y, Arihiro A, Mizoguchi E (2007) Role of mammalian chitinases in inflammatory conditions. Keio J Med 56(1):21–27CrossRefPubMed

Mizoguchi E (2006) Chitinase 3-like-1 exacerbates intestinal inflammation by enhancing bacterial adhesion and invasion in colonic epithelial cells. Gastroenterology 130(2):398–411. https://doi.org/10.1053/j.gastro.2005.12.007 CrossRefPubMed

Lai T, Wu D, Chen M, Cao C, Jing Z, Huang L, Lv Y, Zhao X, Lv Q, Wang Y, Li D, Wu B, Shen H (2016) YKL-40 expression in chronic obstructive pulmonary disease: relation to acute exacerbations and airway remodeling. Respir Res 17:31. https://doi.org/10.1186/s12931-016-0338-3 CrossRefPubMedPubMedCentral

Zhou Y, Peng H, Sun H, Peng X, Tang C, Gan Y, Chen X, Mathur A, Hu B, Slade MD, Montgomery RR, Shaw AC, Homer RJ, White ES, Lee CM, Moore MW, Gulati M, Lee CG, Elias JA, Herzog EL (2014) Chitinase 3-like 1 suppresses injury and promotes fibroproliferative responses in Mammalian lung fibrosis. Sci Transl Med 6(240):240ra276. https://doi.org/10.1126/scitranslmed.3007096 CrossRef

10.

Park SJ, Jun YJ, Kim TH, Jung JY, Hwang GH, Jung KJ, Lee SH, Lee HM, Lee SH (2013) Increased expression of YKL-40 in mild and moderate/severe persistent allergic rhinitis and its possible contribution to remodeling of nasal mucosa. Am J Rhinol Allergy 27(5):372–380. https://doi.org/10.2500/ajra.2013.27.3941 CrossRefPubMed

11.

Rosenfeld RM, Piccirillo JF, Chandrasekhar SS, Brook I, Kumar KA, Kramper M, Orlandi RR, Palmer JN, Patel ZM, Peters A, Walsh SA, Corrigan MD (2015) Clinical practice guideline (update): adult sinusitis executive summary. Otolaryngol Head Neck Surg Off J Am Acad Otolaryngol Head Neck Surg 152(4):598–609. https://doi.org/10.1177/0194599815574247 CrossRef

12.

Wang H, Bai J, Ding M, Liu W, Xu R, Zhang J, Shi J, Li H (2013) Interleukin-17A contributes to the expression of serum amyloid A in chronic rhinosinusitis with nasal polyps. Eur Arch Otorhinolaryngol Off J Eur Fed Otorhinolaryngol Soc (EUFOS) Affil Ger Soc Otorhinolaryngol Head Neck Surg 270(6):1867–1872. https://doi.org/10.1007/s00405-012-2295-x

13.

Van Bruaene N, Derycke L, Perez-Novo CA, Gevaert P, Holtappels G, De Ruyck N, Cuvelier C, Van Cauwenberge P, Bachert C (2009) TGF-beta signaling and collagen deposition in chronic rhinosinusitis. J Allergy Clin Immunol 124(2):253–259, 259.e251–252. https://doi.org/10.1016/j.jaci.2009.04.013

14.

Zhang N, Van Zele T, Perez-Novo C, Van Bruaene N, Holtappels G, DeRuyck N, Van Cauwenberge P, Bachert C (2008) Different types of T-effector cells orchestrate mucosal inflammation in chronic sinus disease. J Allergy Clin Immunol 122(5):961–968. https://doi.org/10.1016/j.jaci.2008.07.008 CrossRefPubMed

15.

Meltzer EO, Hamilos DL, Hadley JA, Lanza DC, Marple BF, Nicklas RA, Bachert C, Baraniuk J, Baroody FM, Benninger MS, Brook I, Chowdhury BA, Druce HM, Durham S, Ferguson B, Gwaltney JM, Kaliner M, Kennedy DW, Lund V, Naclerio R, Pawankar R, Piccirillo JF, Rohane P, Simon R, Slavin RG, Togias A, Wald ER, Zinreich SJ (2004) Rhinosinusitis: establishing definitions for clinical research and patient care. J Allergy Clin Immunol 114(6 Suppl):155–212. https://doi.org/10.1016/j.jaci.2004.09.029 CrossRefPubMed

16.

Lui JK, Lutchen KR (2017) The role of heterogeneity in asthma: a structure-to-function perspective. Clin Transl Med 6(1):29. https://doi.org/10.1186/s40169-017-0159-0 CrossRefPubMedPubMedCentral

17.

Hew M, Bhavsar P, Torrego A, Meah S, Khorasani N, Barnes PJ, Adcock I, Chung KF (2006) Relative corticosteroid insensitivity of peripheral blood mononuclear cells in severe asthma. Am J Respir Crit Care Med 174(2):134–141. https://doi.org/10.1164/rccm.200512-1930OC CrossRefPubMedPubMedCentral

18.

Capone M, Maggi L, Santarlasci V, Rossi MC, Mazzoni A, Montaini G, Cimaz R, Ramazzotti M, Piccinni MP, Barra G, De Palma R, Liotta F, Maggi E, Romagnani S, Annunziato F, Cosmi L (2016) Chitinase 3-like-1 is produced by human Th17 cells and correlates with the level of inflammation in juvenile idiopathic arthritis patients. Clin Mol Allergy CMA 14:16. https://doi.org/10.1186/s12948-016-0053-0 CrossRefPubMed

19.

Specjalski K, Chelminska M, Jassem E (2015) YKL-40 protein correlates with the phenotype of asthma. Lung 193(2):189–194. https://doi.org/10.1007/s00408-015-9693-y CrossRefPubMedPubMedCentral

20.

Lai T, Chen M, Deng Z, L Y, Wu D, Li D, Wu B (2015) YKL-40 is correlated with FEV1 and the asthma control test (ACT) in asthmatic patients: influence of treatment. BMC Pulm Med 15:1. https://doi.org/10.1186/1471-2466-15-1 CrossRefPubMedPubMedCentral

21.

Zhu Z, Zheng T, Homer RJ, Kim YK, Chen NY, Cohn L, Hamid Q, Elias JA (2004) Acidic mammalian chitinase in asthmatic Th2 inflammation and IL-13 pathway activation. Science 304(5677):1678–1682. https://doi.org/10.1126/science.1095336 CrossRefPubMed

Title: The role of YKL40 in the pathogenesis of CRS with nasal polyps
Authors: Yue Ma
Chunquan Zheng
Le Shi
Publication date: 01-02-2018
Publisher: Springer Berlin Heidelberg
Published in: European Archives of Oto-Rhino-Laryngology / Issue 2/2018
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-017-4859-2

At a glance: The STEP trials

Springer Medicine

The role of YKL40 in the pathogenesis of CRS with nasal polyps

Abstract

Background

Objective

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Objective

Methods

Results

Conclusions

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