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BMC Ophthalmology

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Open Access 01-12-2015 | Research article

The role of Syk signaling in antifungal innate immunity of human corneal epithelial cells

Authors: Ying Liu, Guiqiu Zhao, Jing Lin, Cui Li, Qing Li, Chengye Che, Qian Wang, Liting Hu

Published in: BMC Ophthalmology | Issue 1/2015

Abstract

Background

Fungal keratitis is a kind of intractable and sight-threatening diseases. Spleen-tyrosine kinase (Syk) is a non-receptor tyrosine kinase, which plays an important role in the signaling pathway of the receptors. In the current study, we investigate the expression and function of Syk in human corneal epithelial cells with Aspergillus fumigatus (A. fumigatus) infection.

Methods

Cultured telomerase-immortalized human corneal epithelial cells (THCEs) were treated with A. fumigatus hyphae with or without treatment of Syk inhibitors. Activation of Syk and the role of Syk in regulating inflammatory cytokines and chemokines expression were evaluated. The mRNA expression was determined by real time PCR, and protein activation was measured by western blotting.

Results

Syk protein was detected in THCEs, and its activation was enhanced after treatment of A. fumigatus hyphae. Expression of inflammatory cytokines (IL-1β and IL-6) and chemokines (IL-8 and CXCL1) mRNA were significantly increased after stimulation of A. fumigatus hyphae in THCEs. Activation of Syk and expression of IL-1β, IL-6, IL-8 and CXCL1 by A. fumigatus hyphae were blocked by Syk inhibitors.

Conclusion

These findings demonstrate that normal human corneal epithelial cells produce Syk, and Syk activation plays an important role in regulating A. fumigatus hyphae-induced inflammatory responses in THCEs.

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Title: The role of Syk signaling in antifungal innate immunity of human corneal epithelial cells
Authors: Ying Liu
Guiqiu Zhao
Jing Lin
Cui Li
Qing Li
Chengye Che
Qian Wang
Liting Hu
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: BMC Ophthalmology / Issue 1/2015
Electronic ISSN: 1471-2415
DOI: https://doi.org/10.1186/s12886-015-0041-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The role of Syk signaling in antifungal innate immunity of human corneal epithelial cells

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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