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Obesity Surgery

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01-07-2011 | Basic Science Research

The Role of Receptor-Interacting Protein 140 in the Accumulation of Fat in Ovariectomised Rats

Authors: Won-Hsiung Liu, Yen-Mei Lee, Kwok-Keung Lam, Yuh-Fung Chen, Jhi-Joung Wang, Mao-Hsiung Yen, Pao-Yun Cheng

Published in: Obesity Surgery | Issue 7/2011

Abstract

Background

Receptor-interacting protein 140 (RIP140) is a corepressor for nuclear receptors with an important role in the inhibition of energy expenditure. Postmenopausal women have increased white adipose tissue (WAT), and excessive accumulation of adipose tissue (obesity) implies a health risk. The aim of the present work was to investigate the time course of RIP140 expression in WAT during the development of ovariectomy (OVX)-induced obesity in rats.

Methods

OVX was performed in female Sprague–Dawley (SD) rats 8 weeks old. Body weight and food intake were determined once a week. WAT of sham-operated, OVX and OVX plus 17β-estradiol therapy (OVX/E2) female SD rats was weighed and used to analyse RIP140 and uncoupling protein 1 (UCP-1) expression by Western blot.

Results

Food intake and body weight were significantly increased during the 2–8 weeks after OVX. Even though body weight increased until killing, food intake progressively decreased from 9 to 16 weeks after OVX in rats. Meanwhile, increased WAT mass and decreased RIP140 expression in WAT were observed in OVX rats. In contrast, the expression of UCP-1, a key target gene of RIP140, in WAT of OVX rats was significantly higher than in sham-operated rats. All of these alterations caused by OVX were mostly reversed by the replacement of 17β-estradiol.

Conclusions

The down-regulation of RIP140 in WAT may play a compensatory role in OVX-induced obesity in rat.

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Title: The Role of Receptor-Interacting Protein 140 in the Accumulation of Fat in Ovariectomised Rats
Authors: Won-Hsiung Liu
Yen-Mei Lee
Kwok-Keung Lam
Yuh-Fung Chen
Jhi-Joung Wang
Mao-Hsiung Yen
Pao-Yun Cheng
Publication date: 01-07-2011
Publisher: Springer-Verlag
Published in: Obesity Surgery / Issue 7/2011
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI: https://doi.org/10.1007/s11695-010-0241-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The Role of Receptor-Interacting Protein 140 in the Accumulation of Fat in Ovariectomised Rats

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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