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Open Access 01-12-2008 | Short report

The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens

Authors: Steven J Drews, AliReza Eshaghi, Daria Pyskir, Pam Chedore, Ernesto Lombos, George Broukhanski, Rachel Higgins, David N Fisman, Joanne Blair, Frances Jamieson

Published in: Diagnostic Pathology | Issue 1/2008

Abstract

The Seeplex™ TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the Mycobacterium tuberculosis complex (MTBC) targets IS6110 and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specimens, the sensitivity and specificity of this assay is equivalent to a labor-intensive traditional endpoint PCR assay and is more sensitive than a commercial real-time PCR assay.

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Title: The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens
Authors: Steven J Drews
AliReza Eshaghi
Daria Pyskir
Pam Chedore
Ernesto Lombos
George Broukhanski
Rachel Higgins
David N Fisman
Joanne Blair
Frances Jamieson
Publication date: 01-12-2008
Publisher: BioMed Central
Published in: Diagnostic Pathology / Issue 1/2008
Electronic ISSN: 1746-1596
DOI: https://doi.org/10.1186/1746-1596-3-37

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The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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