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Cancer Cell International
Published in: Cancer Cell International 1/2017

Open Access 01-12-2017 | Primary Research

The prognostic values of the expression of Vimentin, TP53, and Podoplanin in patients with cervical cancer

Authors: Jiaying Lin, Jiaqi Lu, Chao Wang, Xiaohong Xue

Published in: Cancer Cell International | Issue 1/2017

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Abstract

Purpose

Epithelial–mesenchymal transition (EMT), TP53, and Podoplanin have been implicated in the tumorigenesis and metastasis of human cancers. Nevertheless, the clinical significance of these markers in cancer patients is still not clear. In this study, we sought to determine the prognostic values of Vimentin, TP53, and Podoplanin in patients with cervical cancer.

Methods

Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis were performed to determine the messenger RNA and protein expression levels of Vimentin, TP53, and Podoplanin, respectively, in cervical squamous cell carcinoma and adjacent normal cervical tissues. Additionally, the expression levels of Podoplanin were also measured in 130 cervical cancer patients (FIGO stages Ib1–IIa2) using immunohistochemistry (IHC) staining.

Results

The mRNA expression levels of Vimentin, TP53, and Podoplanin were considerably elevated in cervical cancer tissues, compared with those in the adjacent normal cervical tissues. Additionally, the protein expression levels of Vimentin were closely correlated with the age of onset (P = 0.007), lymph node metastasis (P = 0.007), lymphatic invasion (P = 0.024), disease recurrence (P < 0.001), and the clinical prognosis of patients with cervical cancer (P < 0.001). Our multivariate analysis also suggests that Vimentin is an independent marker for survival in cervical cancer patients. Furthermore, the expression levels of Vimentin are negatively correlated with the proliferation marker Ki67 expression.

Conclusions

Our data show that Vimentin can serve as an independent prognostic marker for cervical cancer patients with primary surgery.
Registration number ChiCTR-TRC-06000236 Registered 15 December 2006
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Metadata
Title
The prognostic values of the expression of Vimentin, TP53, and Podoplanin in patients with cervical cancer
Authors
Jiaying Lin
Jiaqi Lu
Chao Wang
Xiaohong Xue
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2017
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-017-0450-6

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