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01-12-2020 | Original Article

The neuroprotective effect of betanin in trimethyltin-induced neurodegeneration in mice

Authors: Wachiryah Thong-asa, Supakorn Prasartsri, Nattakan Klomkleaw, Nutnicha Thongwan

Published in: Metabolic Brain Disease | Issue 8/2020

Abstract

Betanin, a natural food colorant with powerful antioxidative properties, has not been studied in terms of neurodegenerative disease intervention. Therefore, the present study aimed to investigate the neuroprotective effects of betanin against trimethyltin chloride (TMT) -induced neurodegeneration in mice. Forty male ICR mice were randomly divided into four groups: Sham-veh, TMT-veh, TMT-Bet50 and TMT-Bet100. In the TMT groups, neurodegeneration was induced with a one-time intraperitoneal injection of 2.6 mg/kg TMT. Betanin-treated groups (Bet) were given oral doses of 50 or 100 mg/kg dissolved in normal saline solution. Administrations were started 24 h prior to TMT injection and continued for 2 weeks. Anxious behavior and spatial cognition were evaluated, respectively. After behavioral tests, brain oxidative status, hippocampal histology and choline acetyltransferase (ChAT) activity were evaluated. Results showed that TMT significant induce anxious behavior and spatial learning and memory deficits (p < 0.05). These were found concurrently with significant decreases in CA1 ChAT activity, brain tissue catalase (CAT) and superoxide dismutase (SOD) activities with significant increase in hippocampal CA1 degeneration (p < 0.05). Betanin 100 mg/kg exhibited significant anxiolytic effect, preventive effect on CA1 degeneration and CA1 ChAT activity alteration as well as improvement of spatial learning and memory deficits (p < 0.05). These were found concurrently with significant increases of reduced glutathione, CAT and SOD activities as well as the decrease in malondialdehyde (p < 0.05). We conclude that betanin 100 mg/kg exhibits neuroprotective effects against TMT-induced neurodegeneration in mice via its anti-oxidative properties, protective against hippocampal CA1 degeneration and ChAT activity alteration. Therefore, betanin is interesting in further neurodegenerative therapeutic study and applications.

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Title: The neuroprotective effect of betanin in trimethyltin-induced neurodegeneration in mice
Authors: Wachiryah Thong-asa
Supakorn Prasartsri
Nattakan Klomkleaw
Nutnicha Thongwan
Publication date: 01-12-2020
Publisher: Springer US
Published in: Metabolic Brain Disease / Issue 8/2020
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-020-00615-1

At a glance: The STEP trials

Springer Medicine

The neuroprotective effect of betanin in trimethyltin-induced neurodegeneration in mice

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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