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Clinical Oral Investigations

Published in:

01-11-2019 | Original Article

The interactions of dendritic cells with osteoblasts on titanium surfaces: an in vitro investigation

Authors: Yang Yang, Xuzhu Wang, Richard J. Miron, Xiaoxin Zhang

Published in: Clinical Oral Investigations | Issue 11/2019

Abstract

Objectives

Osteoimmune interactions possess a critical part in the integration of materials and hosts. Dendritic cells (DCs) are the most common members of osteoimmune cells family. The titanium surfaces of dental implants tend to promote a mature dendritic cell phenotype with increased proinflammatory secretion. However, very little is known to the effects of this microenvironment on the behaviors of cells around implants, especially osteoblasts, and how the tissue integrations take place on such biomaterial surfaces. Therefore, the present study was to investigate the interactions of DCs with osteoblasts on titanium surfaces. DCs seeded on PT and SLA titanium surfaces were compared by assays for the proliferations, surface markers, and inflammatory genes expressions.

Materials and methods

DCs seeded on PT and SLA titanium surfaces were compared by assays for the proliferations, surface markers, and inflammatory genes expressions. Next, we harvested the dendritic cell–conditioned medium (CM) and investigated the effects of CM on MC3T3-E1.

Results

The results showed an increase in CD86 and proinflammatory expressions of DCs seeded on PT and SLA surfaces, as well as a decrease in anti-inflammatory cytokines. The CM from titanium surfaces inhibited the osteoblast differentiation with reduced expression of osteogenic genes RUNX2, COL1, ALP, and OCN and decreased ALP activity as well as Alizarin red staining.

Conclusion

These findings suggested that titanium surfaces switch DCs toward maturation phenotypes and thus inhibit the differentiation and mineralization of osteoblasts.

Clinical relevance

Knowing the significant impact of immune cells on osteogenesis behaviors, some efforts to decrease the immune reaction might be of clinical significance. Favorable immune environments can increase the dental implants survival rate in patients.

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Title: The interactions of dendritic cells with osteoblasts on titanium surfaces: an in vitro investigation
Authors: Yang Yang
Xuzhu Wang
Richard J. Miron
Xiaoxin Zhang
Publication date: 01-11-2019
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 11/2019
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-02852-w

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The interactions of dendritic cells with osteoblasts on titanium surfaces: an in vitro investigation

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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