Top

Published in:

01-08-2011 | Original Article

The IL-1β (+3953 T/C) gene polymorphism associates to symptomatic lumbar disc herniation

Authors: J. Paz Aparicio, I. Fernández Bances, E. López-Anglada Fernández, A. H. Montes, A. Paz Aparicio, J. Pena Vázquez, S. Ramos García, S. Antón García, P. López Fernández, E. Valle-Garay, V. Asensi

Published in: European Spine Journal | Special Issue 3/2011

Abstract

Purpose

To determine whether polymorphisms (SNPs) in the genes encoding cytokines and nitric oxide synthase (NOS) might play some role in lumbar disc herniation (LDH).

Patients and methods

Case–control study in which 179 patients were retrospectively reviewed. The case group was made of 50 patients with symptomatic LDH diagnosed by MRI while the control group was made of 129 individuals undergoing routine hip or knee arthroplasty with a lifetime lack of low back pain. SNPs in the cytokine genes of IL-1 [IL-1α (−889 C/T), IL-1β (+3953 T/C)], TNF-α (−308 G/A and −238 G/A) and NOS genes [eNOS (r 27 bp, intron 4 and −786 T/C) and iNOS (22 G/A)].

Results

The CC genotype and C allele of the IL-1β (+3953 T/C) SNP were significantly more frequent among LDH patients compared to controls. On the other hand, eNOS (−768 T/C) and iNOS (22 G/A) SNPs were significantly more common in the control group.

Conclusions

Carriers of the CC genotype of the IL-1β (+3953 T/C) SNP were more frequent among LDH patients suggesting some potential role of the IL-1β SNP on LDH pathogenesis. The eNOS (−786 T/C) and iNOS (22 G/A) SNPs were more frequent among the control subjects, suggesting their possible protective role against LDH. Genotyping these SNPs could be useful to identify persons with an increased lifetime risk of disc herniation in whom measures to avoid LDH could be implemented.

Mok FPS, Samartzis D, Karppinen J, Luk KDK, Fong DYT, Cheung KMC (2010) Prevalence, determinants, and association of Schmorl nodes of the lumbar spine with disc degeneration. A population-based study of 2449 individuals. Spine 35:1944–1952PubMedCrossRef

Arana E, Royuela A, Kovacs FM, Estremera A, Sarasibar H, Amengual G, Galarraga I, Martinez C, Muriel A, Abraira V, Gil Del Real MT, Zamora J, Campillo C (2010) Lumbar spine: agreement in the interpretation of 1.5-T MR images by using the Nordic Modic Consensus Group classification form. Radiology 254:809–817PubMedCrossRef

Tong HC, Carson JT, Haig AJ, Quint DJ, Phalke VR, Yamakawa KSJ, Miner JA (2006) Magnetic resonance imaging of the lumbar spine in asymptomatic older adults. J Back Musculoskeletal Rehabil 19:67–72

Azpeitia AJ, Martinez SM, Céspedes MM, Rebolledo VM, Millan JJM (1992) Tomografía computarizada de las hernias discales foraminales y extraforaminales. Radiología 34:345–349

McGregor AJ, Andrew T, Sambrook PN, Spector TD (2004) Structural, psychological and genetic influences on low back and neck pain: a study of adult female twins. Arthritis Rheum 51:160–167CrossRef

Patel AA, Spiker WR, Daubs M, Brodke D, Cannon-Albright LA (2011) Evidence for an inherited predisposition to lumbar disc disease. J Bone Surg Am 93:225–229PubMedCrossRef

Schumann B, Bolm.-Audorff U, Bergmann A, Elegast R, Elsner G, Grifka J, Hearting J, Jäger M, Michaelis M, Seidler A (2011) Lifestyle factors and lumbar disc disease: results of a German multi-center case-control study (EPILIFT). Arthritis Res Ther 12:R193CrossRef

Kim KS, Yoon ST, Park JS, Li J, Park MS, Hutton WC (2003) Inhibition of proteoglycan and type II collagen synthesis of disc nucleus cells by nicotine. J Neurosurg Spine 99:291–297CrossRef

Solavieva S, Kouhia S, Leino-Arjas P, Ala-Kokko L, Luoma K, Raininko R, Saarela J, Riihimäki H (2004) Interleukin-1 polymorphisms and intervertebral disc degeneration. Epidemiology 15:626–633CrossRef

10.

Le Maitre CL, Freemont AJ, Hoyland JA (2005) The role of interleukin-1 in the pathogenesis of human intervertebral disc degeneration. Arthritis Res Ther 7:R732–R745PubMedCrossRef

11.

Evans CH, Stefanovic-Racic M, Lancaster J (1995) Nitric oxide and its role in orthopaedic disease. Clin Orthop Rel Res 312:275–294

12.

Stea S, Visentin M, Donati ME, Granchi D, Ciapetti G, Sudanese A, Toni A (2002) Nitric oxide synthase in tissues around failed hip prostheses. Biomaterials 23:4833–4838PubMedCrossRef

13.

Liu GZ, Ishihara H, Osada R, Kimura T, Tsuji H (2001) Nitric oxide mediates the change of proteoglycan synthesis in the human lumbar intervertebral disc in response to hydrostatic pressure. Spine 26:134–141PubMedCrossRef

14.

UCSF Medical Center. Spine Center. Lumbar disc herniation. Available at: http://www.ucsfhealth.org/conditions/lumbar_disc_herniation/

15.

Miller SA, Dykes DD, Polesky HF (1988) A simple salting-out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res 16:1215PubMedCrossRef

16.

Takahashi H, Suguro T, Okazima Y, Motegi M, Okada Y, Kakiuchi T (1996) Inflammatory cytokines in the herniated disc of the lumbar spine. Spine 21:218–224 (Phila Pa 1976)PubMedCrossRef

17.

Solavieva S, Leino-Arjas P, Saarela J, Luoma K, Raininko R, Riihimäki H (2004) Possible association of interleukin 1 gene locus polymorphisms with low back pain. Pain 109:8–19CrossRef

18.

Solovieva S, Lohiniva J, Leino-Arjas P, Raininko R, Luoma K, Ala-Kokko L, Riihimäki H (2006) Intervertebral disc degeneration in realtion to the COLA9A3 and the IL-1ss gene polymorphism. Eur Spine J 15:613–619PubMedCrossRef

19.

Virtanen IM, Karppinen J, Taimela S, Ott J, Barral S, Kaikkonen K, Heikkilä O, Mutanen P, Noponen N, Männikkö M, Tervonen O, Natri A, Ala-Kokko L (2007) Occupational and genetic risk factors associated with intervertebral disc disease. Spine 32:1129–1134PubMedCrossRef

20.

Solovieva S, Noponen N, Männikkö M, Leino-Arjas P, Luoma K, Raininko R, Ala-Kokko L, Riihimäki H (2007) Association between the aggrecan gene variable number of tandem repeats polymorphism and intervertebral disc degeneration. Spine 32:1700–1705PubMedCrossRef

21.

Ye W, Ma RF, Su PQ, Huang DS, Liu SL, Chen WJ, Wang XG (2007) Association of single nucleotide polymorphisms of IL-1β with lumbar disc disease. Yi Chuan. 29:923–928PubMed

22.

Pociot F, Molvig J, Wogensen L, Worsaae H, Nerup J (1992) A TaqI polymorphism in the human interleukin-1β (IL-1β) gene correlates withIL-1β secretion in vitro. Eur J Clin Invest 22:396–402PubMedCrossRef

23.

Valdes AM, Hassett G, Hart DJ, Spector TD (2005) Radiographic progression of lumbar spine disc degeneration is influenced by variation at inflammatory genes: a candidate SNP association study in the Chingford cohort. Spine 30:2445–2451PubMedCrossRef

24.

Yuan HY, Tang Y, Liang YX, Lei L, Xiao GB, Wang S, Xia ZL (2010) Matrix metalloproteinase-3 and vitamin D receptor genetic polymorphisms, and their interactions with occupational exposure in lumbar disc degeneration. J Occup Health 52:23–30PubMedCrossRef

25.

Videman T, Saarela J, Kaprio J, Näkki A, Levälahti E, Gill K, Peltonen L, Battié MC (2009) Associations of 25 structural, degradative, and inflammatory candidate genes with lumbar disc desiccation, bulging, and height narrowing. Arthritis Rheum 60:470–481PubMedCrossRef

26.

Karasugi T, Semba K, Hirose Y, Kelempisioti A, Nakajima M, Miyake A, Furuichi T, Kawaguchi Y, Mikami Y, Chiba K, Kamata M, Ozaki K, Takahashi A, Mäkelä P, Karppinen J, Kimura T, Kubo T, Toyama Y, Yamamura K, Männikkö M, Mizuta H, Ikegawa S (2009) Association of the tag SNPs in the human SKT gene (KIAA1217) with lumbar disc herniation. J Bone Miner Res 24:1537–1543PubMedCrossRef

27.

Solovieva S, Lohiniva J, Leino-Arjas P, Raininko R, Luoma K, Ala-Kokko L, Riihimäki H (2002) COL9A3 gene polymorphism and obesity in intervertebral disc degeneration of the lumbar spine: evidence of gene-environment interaction. Spine 27:2691–2696PubMedCrossRef

Title: The IL-1β (+3953 T/C) gene polymorphism associates to symptomatic lumbar disc herniation
Authors: J. Paz Aparicio
I. Fernández Bances
E. López-Anglada Fernández
A. H. Montes
A. Paz Aparicio
J. Pena Vázquez
S. Ramos García
S. Antón García
P. López Fernández
E. Valle-Garay
V. Asensi
Publication date: 01-08-2011
Publisher: Springer-Verlag
Published in: European Spine Journal / Issue Special Issue 3/2011
Print ISSN: 0940-6719
Electronic ISSN: 1432-0932
DOI: https://doi.org/10.1007/s00586-011-1915-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The IL-1β (+3953 T/C) gene polymorphism associates to symptomatic lumbar disc herniation

Abstract

Purpose

Patients and methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Patients and methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Special Issue 3/2011

Vertebral growth modulation by hemicircumferential electrocoagulation: an experimental study in pigs

Individualized antibiotic prophylaxis reduces surgical site infections by gram-negative bacteria in instrumented spinal surgery

Evaluation of autologous platelet concentrate for intertransverse lumbar fusion

Beneficial effects of aerobic training in adolescent patients with moderate idiopathic scoliosis

Biomechanical, histological and histomorphometric analyses of calcium phosphate cement compared to PMMA for vertebral augmentation in a validated animal model

Impact of magnetic resonance imaging on decision making for thoracolumbar traumatic fracture diagnosis and treatment