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Journal of Experimental & Clinical Cancer Research

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Open Access 01-12-2011 | Research

The function and mechanism of COX-2 in angiogenesis of gastric cancer cells

Authors: Liping Yao, Fei Liu, Liu Hong, Li Sun, Shuhui Liang, Kaichun Wu, Daiming Fan

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2011

Abstract

Background

Here we aimed to investigate the effect of COX-2 siRNA on proliferation and angiogenesis of gastric cancer cells.

Methods

The gastric cancer cell line SGC7901 was transfected with COX-2 siRNA, then the growth and angiogenesis of cells were detected by in vitro and in vivo assay. Human microarray, RT-PCR and western blot were used to identify differentially expressed angiogenesis-related molecules in cells with decreased expression of COX-2.

Results

Down-regulation of COX-2 could significantly inhibit the in vitro and in vivo growth of gastric cancer cells, and suppress the migration and tube formation of human umbilical vein endothelial cells. Totally 23 angiogenesis-related molecules were found involved in COX-2-induced angiogenesis suppression. The results of RT-PCR and western blot showed that down-regulation of COX-2 might inhibit VEGF, Flt-1, Flk-1/KDR, angiopoietin-1, tie-2, MMP2 and OPN.

Conclusions

COX-2 might mediate tumor angiogenesis and growth, and could be considered as a target for gastric cancer therapy.

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Title: The function and mechanism of COX-2 in angiogenesis of gastric cancer cells
Authors: Liping Yao
Fei Liu
Liu Hong
Li Sun
Shuhui Liang
Kaichun Wu
Daiming Fan
Publication date: 01-12-2011
Publisher: BioMed Central
Published in: Journal of Experimental & Clinical Cancer Research / Issue 1/2011
Electronic ISSN: 1756-9966
DOI: https://doi.org/10.1186/1756-9966-30-13

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Abstract

Background

Methods

Results

Conclusions

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