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01-02-2006 | Original Article

The effects of exogenous interleukin-4 on hypoxia-induced lung injury

Authors: Hayrettin Ozturk, Hulya Ozturk, Huseyin Buyukbayram, Mehmet Cudi Tuncer

Published in: Pediatric Surgery International | Issue 2/2006

Abstract

The aim of this study was to determine the effects of recombinant human interleukin-4 (rhIL-4) on hypoxia-induced lung injury in immature rats. The study was performed on 1-day-old Sprague Dawley rat pups. Group 1 (n=7) served as nonhypoxic controls. Group 2 (untreated, n=7) rats were subjected to hypoxia–reoxygenation (H/O) and were then returned to their mothers. Group 3 (rhIL-4 treated, n=7) rats were subjected to H/O, were returned to their mothers, and were treated with rhIL-4 (75 μg/kg subcutaneously) for the next 3 days. All animals were killed on day 4 and lung specimens were obtained to determine the tissue level of malondialdehyde (MDA) and histological changes. In the untreated group, MDA levels were significantly increased compared to control and rhIL-4 groups. In the rhIL-4 treated group, MDA levels were not significantly different compared to control group. The rhIL-4 treated group had a significantly lower score of pathological lesions and alveolar hemorrhage grade values than the untreated group. The untreated animals had areas marked intraalveolar hemorrhage, capillary congestion, interstitial edema, and hypercellularity of lung that were not seen in the rhIL-4 treated rats. In conclusion, this study found beneficial effects of rhIL-4 in an experimental model on the hypoxia-induced lung injury.

Hashimoto N, Takeyoshi I, Tsutsumi H, Sunose Y et al (2002) Effects of a bradykinin B(2) receptor antagonist, FR173657, on pulmonary ischemia–reperfusion injury in dogs. J Heart Lung Transplant 21:1022–1029PubMedCrossRef

Naka Y, Roy DK, Smerling AJ et al (1995) Inhaled nitric oxide fails to confer the pulmonary protection provided by distal stimulation of the nitric oxide pathway at the level of cyclic guanosine monophosphate. J Thorac Cardiovasc Surg 110:1434–1441PubMedCrossRef

Krishnadasan B, Farivar AS, Naidu BV et al (2004) Beta-chemokine function in experimental lung ischemia–reperfusion injury. Ann Thorac Surg 77:1056–1062PubMedCrossRef

Pham S, Yoshida Y, Aeba R et al (1992) Interleukin-6, a marker for preservation injury in clinical lung transplantation. J Heart Lung Transplant 11:1017–1022PubMed

Eppinger MJ, Ward PA, Bolling SF, Deeb GM (1996) Regulatory effects of interleukin-10 on lung ischemia reperfusion injury. J Thorac Cardiovasc Surg 112:1301–1306PubMedCrossRef

Stammberger U, Carboni GL, Schneiter D et al (1999) Combined treatment with endothelin and PAF antagonists reduces post-transplant lung ischemia/reperfusion injury. J Heart Lung Transplant 18:862–868PubMedCrossRef

Farivar AS, Krishnadasan B, Naidu BV et al (2003) Endogenous interleukin-4 and interleukin-10 regulate experimental lung ischemia reperfusion injury. Ann Thorac Surg 76:253–259PubMedCrossRef

Chernoff AE, Granowitz EV, Shapiro L et al (1995) A randomized controlled trial of IL-10 in Humans. Inhibition of inflammatory cytokine production and immune responses. J Immunol 154:5492–5499PubMed

Freimann J, Estrov Z, Itoh K (1991) Phase I studies of recombinant human Interleukin 4. Proc Am Soc Clin Oncol 725:216–228

10.

Okur H, Küçükaydin M, Köse K et al (1995) Hypoxia-induced necrotizing entero-colitis in the immature rat: The role of lipid peroxidation and management by vitamin E. J Pediatr Surg 30:1416–1419PubMedCrossRef

11.

Ozturk H, Dokucu AI, Ogun C, Buyukbayram H (2002) Protective effects of recombinant human interleukin-10 on intestines of hypoxia-induced necrotizing enterocolitis in immature rats. J Pediatr Surg 37:1330–1333PubMedCrossRef

12.

Higgins RSD, Letsou GV, Sanchez JA, Eisen RN, Smith GJW, Franco KL (1993) Improved ultrastructural lung preservation with prostaglandin E1 as donor pretreatment in a primate model of heart–lung transplantation. J Thorac Cardiovasc Surg 105:956–971

13.

Egan TM, Lambert CJ, Reddick R et al (1991) A strategy to increase the donor pool: use of cadaver lungs for transplantation. Ann Thorac Surg 52:1113–1121PubMedCrossRef

14.

Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358PubMedCrossRef

15.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275PubMed

16.

Eren S, Esme H, Balci AE et al (2003) The effect of aprotinin on ischemia–reperfusion injury in an in situ normothermic ischemic lung model. Eur J Cardiothorac Surg 23:60–65PubMedCrossRef

17.

Roberts RF, Nishanian GP, Carey JN et al (1998) Addition of aprotinin to organ preservation solutions decreases lung reperfusion injury (see comments). Ann Thorac Surg 66:225–230PubMedCrossRef

18.

Martin DR, Scott F, Downes GL, Belzer FO (1972) Primary cause of unsuccessful liver and heart preservation: cold sensitivity of the ATPase system. Ann Surg 175:111–117PubMedCrossRef

19.

Huber TS, Gaines GC, Welborn MB et al (2000) Anticytokine therapies for acute inflammation and the systemic inflammatory response syndrome: IL-10 and ischemia reperfusion injury as a new paradigm. Shock 13:425–434PubMedCrossRef

20.

Freimann J, Estrov Z, Itoh K (1991) Phase I studies of recombinant human Interleukin 4. Proc Am Soc Clin Oncol 725:216–228

21.

Naidu BV, Farivar AS, Woolley SM et al (2004) Novel broad-spectrum chemokine inhibitor protects against lung ischemia–reperfusion injury. J Heart Lung Transplant 23:128–134PubMedCrossRef

22.

Bless NM, Huber-Lang M, Guo RF et al (2000) Role of CC chemokines in acute lung injury in rats. J Immunol 164:2650–2659PubMed

23.

Lentsch AB, Yoshidome H, Cheadle WG et al (1998) Chemokine involvement in hepatic ischemia/reperfusion injury in mice: roles for macrophage inflammatory protein-2 and KC. Hepatology 27:1172–1177PubMedCrossRef

24.

Miura M, Fu X, Zhang QW, Remick DG et al (2001) Neutralization of Gro and MIP-2 attenuates renal ischemia/reperfusion injury. Am J Pathol 159:2137–2145PubMed

25.

Matsumoto T, Ikeda K, Mukaida N et al (1997) Prevention of cerebral edema and infarct in cerebral reperfusion injury by an antibody to interleukin-8. Lab Invest 77:119–125PubMed

26.

Yagihashi A, Tsuruma T, Tarumi K et al (1998) Prevention of small intestinal ischemia–reperfusion injury in rat by anti-cytokine-induced neutrophil chemoattractant monoclonal antibody. J Surg Res 78:92–96PubMedCrossRef

27.

Hefner EJ, Repine EJ (1989) Pulmonary strategies of antioxidant defense. Am Rev Respir Dis 140:531–554

28.

Schmidt R, Schafer C, Luboeinski T et al (2004) Increase in alveolar antioxidant levels in hyperoxic and anoxic ventilated rabbit lungs during ischemia. Free Radic Biol Med 36:78–89PubMedCrossRef

29.

Vaughan WG, Horton JW, Walker PB (1992) Allopurinol prevents intestinal permeability changes after ischemia–reperfusion injury. J Pediatr Surg 27:968–973PubMedCrossRef

30.

Caplan MS, Sun XM, Hsueh W (1990) Hypoxia causes ischemic bowel necrosis in rats: the role of platelet-activating factor (PAF-acether). Gastroenterology 99:979–986PubMed

Title: The effects of exogenous interleukin-4 on hypoxia-induced lung injury
Authors: Hayrettin Ozturk
Hulya Ozturk
Huseyin Buyukbayram
Mehmet Cudi Tuncer
Publication date: 01-02-2006
Publisher: Springer-Verlag
Published in: Pediatric Surgery International / Issue 2/2006
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-006-1635-6

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The effects of exogenous interleukin-4 on hypoxia-induced lung injury

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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