Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2014

Open Access 01-12-2014 | Research article

The effects of Cordyceps sinensis phytoestrogen on estrogen deficiency-induced osteoporosis in Ovariectomized rats

Authors: Da-wei Zhang, Zhen-lin Wang, Wei Qi, Guang-yue Zhao

Published in: BMC Complementary Medicine and Therapies | Issue 1/2014

Login to get access

Abstract

Background

Isoflavones are naturally occurring plant chemicals belonging to the “phytoestrogen” class. The aim of the present study was to examine the effects of isoflavones obtained from Cordyceps sinensis (CSIF) on development of estrogen deficiency-induced osteoporosis in ovariectomized rats.

Methods

After the rats were treated orally with CSIF, serum alkaline phosphatase (ALP), tartarate resistant acid phosphatase (TRAP), serum osteocalcin (OC), homocysteine (HCY), C-terminal crosslinked telopeptides of collagen type I (CTX), estradiol and interferonγ (IFN-γ) level were examined. At the same time, the urine calcium, plasma calcium, plasma phosphorus and the mass of uterus, thymus and body were also examined.

Results

The beneficial effects of CSIF on improvement of osteoporosis in rats were attributable mainly to decrease ALP activity, TRAP activity, CTX level and IFN-γ level. At the same time, CSIF also increase the OC and estradiol level in ovariectomized osteopenic rats. The histological examination clearly showed that dietary CSIF can prevent bone loss caused by estrogen deficiency.

Conclusion

The significant estrogenic activity of CSIF demonstrated that CSIF has significant estrogenic effects in OVX rats.
Appendix
Available only for authorised users
Literature
1.
Zhu JS, Halpern GM, Jones K: The scientific rediscovery of an ancient Chinese herbal medicine. Cordyceps sinensis. J Altern Complement Med. 1998, 4: 289-303. 10.1089/acm.1998.4.3-289.CrossRefPubMed
2.
Kuo YC, Tsai WJ, Shiao MS, Chen CF, Lin CY: Cordyceps sinensis as an immunomodulatory agent. Am J Chin Med. 1996, 24: 111-125. 10.1142/S0192415X96000165.CrossRefPubMed
3.
Yang LY, Chen A, Kuo YC, Lin CY: Efficacy of a pure compound H1-A extracted from Cordyceps sinensis on autoimmune disease of MRL lpr/lpr mice. J Lab Clin Med. 1999, 134: 492-500. 10.1016/S0022-2143(99)90171-3.CrossRefPubMed
4.
Kuo YC, Tsai WJ, Wang JY, Chang SC, Lin CY, Shiao MS: Regulation of bronchoalveolar lavage fluids cell function by the immunomodulatory agents from Cordyceps sinensis. Life Sci. 2001, 68: 1067-1082. 10.1016/S0024-3205(00)01011-0.CrossRefPubMed
5.
Kuo YC, Lin CY, Tsai WJ, Wu CL, Chen CF, Shiao MS: Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides. Cancer Invest. 1994, 12: 611-615. 10.3109/07357909409023046.CrossRefPubMed
6.
Bok JW, Lermer L, Chilton J, Klingeman HG, Towers GH: Antitumor sterols from the mycelia of Cordyceps sinensis. Phytochemistry. 1999, 51: 891-898. 10.1016/S0031-9422(99)00128-4.CrossRefPubMed
7.
Manabe N, Azuma Y, Sugimoto M, Uchio K, Miyamoto M, Taketomo N, Tsuchita H, Miyamoto H: Effects of the mycelial extract of cultured Cordyceps sinensis on in vivo hepatic energy metabolism and blood flow in dietary hypoferric anaemic mice. Br J Nutr. 2000, 83: 197-204.CrossRefPubMed
8.
Balon TW, Jasman AP, Zhu JS: A fermentation product of Cordyceps sinensis increases whole-body insulin sensitivity in rats. J Altern Complement Med. 2002, 8: 315-323.CrossRefPubMed
9.
Koh JH, Kim JM, Chang UJ, Suh HJ: Hypocholesterolemic effect of hot-water extract from mycelia of Cordyceps sinensis. Biol Pharm Bull. 2003, 26: 4-87.
10.
Chiou WF, Chang PC, Chou CJ, Chen CF: Protein constituent contributes to the hypotensive and vasorelaxant activities of Cordyceps sinensis. Life Sci. 2000, 66: 1369-1376. 10.1016/S0024-3205(00)00445-8.CrossRefPubMed
11.
Qi W, Yan YB, Wang PJ, Lei W: The Co-effect of Cordyceps sinensis and Strontium on Osteoporosis in Ovariectomized Osteopenic Rats. Biol Trace Elem Res. 2010, 141 (1–3): 216-223.PubMed
12.
Messina MJ, Persky V, Setchell KD, Barnes S: Soy intake and cancer risk: a review of the in vitro and in vivo data. Nutr Cancer. 1994, 21: 113-131. 10.1080/01635589409514310.CrossRefPubMed
13.
Van der Schouw YT, de Kleijn MJ, Peeters PH, Grobbee DE: Phyto-oestrogens and cardiovascular disease risk. Nutr Metab Cardiovasc Dis. 2000, 10: 154-167.PubMed
14.
Webb CM, Hayward CS, Mason MJ, Llsley CD, Collins P: Coronary vasomotor and blood flow responses to isoflavone-intact soya protein in subjects with coronary heart disease or risk factors for coronary heart disease. Clin Sci (London). 2008, 115: 353-359. 10.1042/CS20070443.CrossRef
15.
Price KR, Fenwick GR: Naturally occurring oestrogens in foods—a review. Food Addit Contam. 1985, 2 (2): 73-106. 10.1080/02652038509373531.CrossRefPubMed
16.
Setchell KD, Cassidy A: Dietary isoflavones: biological effects and relevance to human health. J Nutr. 1999, 129 (3): 758S-767S.PubMed
17.
Waldman TN: Menopause: when hormone replacement therapy is not an option. Part II. J Women Health. 1998, 7: 673-683. 10.1089/jwh.1998.7.673.CrossRef
18.
Barnabei VM, Grady D, Stovall DW, Cauley JA, Lin F, Stuenkel CA, Stefanick ML, Pickar JH: Menopausal symptoms in older women and the effects of treatment with hormone therapy. Obstet Gynecol. 2002, 100: 1209-1218. 10.1016/S0029-7844(02)02369-4.PubMed
19.
Choi JN, Kim J, Lee MY, Park DK, Hong YS, Lee CH: Metabolomics revealed novel isoflavones and optimal cultivation time of Cordyceps militaris fermentation. J Agric Food Chem. 2010, 58: 4258-4267. 10.1021/jf903822e.CrossRefPubMed
20.
Weiss J, Taylor GR, Zimmermann F, Nebendahl K: Collection of Body Fluids. Laboratory Rat Book. 2000, New York: Academic Press, 485-510.CrossRef
21.
Changrani NR, Chonkar A, Adeghate E: Effects of streptozotocininduced type 1 diabetes mellitus on total protein concentrations and cation contents in the isolated pancreas, parotid, submandibular, and lacrimal glands of rats. Ann N Y Acad Sci. 2006, 1084: 503-519. 10.1196/annals.1372.019.CrossRefPubMed
22.
Bessy OA, Lowry OH, Brock MJ: A method for the rapid determination of alkaline phosphatase with five cubic millimetres of serum. J Biol Chem. 1946, 164: 321-329.
23.
Godkar P: Enzymes. Textbook of Medical Laboratory Techniques, Ed. 1994, Mumbai, India: Bhalani Publishing House, 149-167.
24.
Repo MA, Bockman RS, Betts F, Boskey AL, Alcock NW, Warrell RP: Effect of gallium on bone mineral properties. Calcif Tissue Int. 1988, 43 (5): 300-306. 10.1007/BF02556640.CrossRefPubMed
25.
Cho PW, Fox JL, Higuchi WI, Pithayanukul P: Influence of dodecylamine hydrochloride adsorption on the dissolution kinetics of hydroxyapatite. Adsorption on and surface chemistry of hydroxyapatite. Edited by: Misra DN. 1984, New York: Plenum Press, 51-70.CrossRef
26.
Mueller M, Hobiger S, Jungbauer A: Red clover extract: a source for substances that activate peroxisome proliferator-activated receptor alpha and ameliorate the cytokine secretion profile of lipopolysaccharide-stimulated macrophages. Menopause. 2010, 17 (2): 379-387. 10.1097/gme.0b013e3181c94617.CrossRefPubMed
27.
Mueller M, Jungbauer A: Red clover extract: a putative source for simultaneous treatment of menopausal disorders and the metabolic syndrome. Menopause. 2008, 15 (6): 1120-1131. 10.1097/gme.0b013e31817062ce.CrossRefPubMed
28.
Medjakovic S, Mueller M, Jungbauer A: Potential health-modulating effects of isoflavones and metabolites via activation of PPAR and AhR. Nutrients. 2010, 2 (3): 241-279. 10.3390/nu2030241.CrossRefPubMedPubMedCentral
29.
Carlson S, Peng N, Prasain JK, Wyss JM: Effects of botanical dietary sup-plements on cardiovascular, cognitive, and metabolic function in males and females.Gender. Medicine. 2008, 5 (Suppl. 1): S76-S90.
30.
Qiu L, Ye H, Chen L, Hong Y, Zhong F, Zhang T: Red clover extract ameliorates dyslipidemia in streptozotocin-induced diabetic C57BL/6 mice by activating hepatic PPAR. Phytother Res. 2012, 26 (6): 860-864. 10.1002/ptr.3641.CrossRefPubMed
31.
Kalaiselvan V, Kalaivani M, Vijayakumar A, Sureshkumar K, Venkatesku-mar K: Current knowledge and future direction of research on soy isoflavones as a therapeutic agents. Pharmacogn Rev. 2010, 4 (8): 111-117. 10.4103/0973-7847.70900.CrossRefPubMedPubMedCentral
32.
Tsunoo A, Taketomo N, Tsuboi H, Kamijo M, Nemoto A, Sasaki H, Uchida M, Meiji institute of Health Science: Cordyceps Sinensis: Its diverse effects on mammals in vitro and in vivo. Third International Symposium of the Mycological Society of Japan. 1995
33.
Yao W, Tian XY, Chen J, Setterberg RB, Lundy MW, Chmielzwski P, Froman CA, Jee WS: Rolipram, a phosphodiesterase 4 inhibitor, prevented cancellous and cortical bone loss by inhibiting endosteal bone resorption and maintaining the elevated periosteal bone formation in adult ovariectomized rats. J Musculoskelet Neuronal Interact. 2007, 7 (2): 119-130.PubMed
34.
Diel P, Schulz T, Smolnikar K, Strunck E, Vollmer G, Michna H: Ability of xeno- and phytoestrogens to modulate expression of estrogen-sensitive genes in rat uterus: estrogenicity profiles and uterotropic activity. J Steroid Biochem Mol Biol. 2000, 73: 1-10. 10.1016/S0960-0760(00)00051-0.CrossRefPubMed
35.
Tomita A: Osteoporosis-blood biochemistry. Jpn J Clin Med. 1990, 48: 2838-2844.
36.
Arai K, Komura H, Akikusa T, Iio K, Kishi H, Watanabe G, Taya K: Contributions of endogenous inhibin and estradiol to the regulation of follicle-stimulating hormone and luteinizing hormone secretion in the pregnant rat. Biol Reprod. 1997, 56: 1482-1489. 10.1095/biolreprod56.6.1482.CrossRefPubMed
37.
Havill LM, Hale LG, Newman DE, Witte SM, Mahaney MC: Bone ALP and OC reference standards in adult baboons (Papio hamadryas) by sex and age. J Med Primatol. 2006, 35: 97-105. 10.1111/j.1600-0684.2006.00150.x.CrossRefPubMed
38.
Ross PD, Knowlton W: Rapid bone loss is associated with increased levels of biochemical markers. J Bone Miner Res. 1998, 13: 297-302. 10.1359/jbmr.1998.13.2.297.CrossRefPubMed
39.
Garnero P, Sornay-Rendu E, Claustrat B, Delmas PD: Biochemical markers of bone turnover, endogenous hormones and the risk of fractures in postmenopausal women: the OFELY study. J Bone Miner Res. 2000, 15: 1526-1536. 10.1359/jbmr.2000.15.8.1526.CrossRefPubMed
40.
Ross PD, Kress BC, Parson RE, Wasnich RD, Armour KA, Mizrahi IA: Serum bone alkaline phosphatase and calcaneus bone density predict fractures: A prospective study. Osteoporos Int. 2000, 11: 76-82. 10.1007/s001980050009.CrossRefPubMed
41.
Polak-Jonkisz D, Zwolinska D: Osteocalcin as a biochemical marker of bone turnover. Nephrology. 1998, 4: 339-346. 10.1111/j.1440-1797.1998.tb00369.x.CrossRef
42.
Burgeson RE: New collagens, new concepts. Annu Rev Cell Biol. 1988, 4: 552-577.CrossRef
43.
Guerrero R, Diaz Martin MA, Diaz Diego EM, Disla T, Rapado A, De la Piedra C: New biochemical markers of bone resorption derived from collagen breakdown in the study of postmenopausal osteoporosis. Osteoporos Int. 1996, 6: 297-302.CrossRefPubMed
44.
Madyastha PR, Yang S, Ries WL, Key LL: IFN-gamma enhances osteoclast generation in cultures of peripheral blood from osteopetrotic patients and normalizes superoxide production. J. Interferon Cytokine Res. 2000, 20: 645-652. 10.1089/107999000414826.CrossRef
Metadata
Title
The effects of Cordyceps sinensis phytoestrogen on estrogen deficiency-induced osteoporosis in Ovariectomized rats
Authors
Da-wei Zhang
Zhen-lin Wang
Wei Qi
Guang-yue Zhao
Publication date
01-12-2014
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2014
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-14-484

Other articles of this Issue 1/2014

BMC Complementary Medicine and Therapies 1/2014 Go to the issue

Research article

Gamma-tocotrienol and hydroxy-chavicol synergistically inhibits growth and induces apoptosis of human glioma cells

Research article

Evaluation of antinociceptive activity of hydromethanol extract of Cyperus rotundus in mice

Research article

A legume product fermented by Saccharomyces cerevisiae modulates cutaneous atopic dermatitis-like inflammation in mice

Research article

Antihyperglycemic action of rhodiola-aqeous extract in type1-like diabetic rats

Research article

Genotoxicity evaluation of Guibi-Tang extract using an in vitro bacterial reverse mutation assay, chromosome aberration assay, and in vivo micronucleus test

Research article

Anti-inflammatory effects of sargachromenol-rich ethanolic extract of Myagropsis myagroides on lipopolysaccharide-stimulated BV-2 cells