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01-06-2008 | Original Paper

The Effects of Caffeic Acid Phenethyl Ester (CAPE) on TNBS-induced Colitis in Ovariectomized Rats

Authors: Rauf Onur Ek, Mukadder Serter, Kemal Ergin, Yuksel Yildiz, Serpil Cecen, Tulay Kavak, Cigdem Yenisey

Published in: Digestive Diseases and Sciences | Issue 6/2008

Abstract

Aim The aim of this investigation was to examine the effects of caffeic acid phenethyl ester (CAPE) on the development of colitis and antioxidant parameters in bilateral ovariectomized rats subjected to trinitrobenzene sulfonic acid (TNBS)-induced colitis. Materials and methods Twenty-one Wistar Albino ovariectomized female rats were divided into four subgroups (n = 5 or 6) (colitis control, vehicle control, CAPE 10 and 30 mg/kg, respectively). Colitis was induced using an enema of TNBS and ethanol, following which CAPE was administrated for 3 days to induce colitis and effect of CAPE was subsequently evaluated. Results Based on microscopic damage scores, there was no difference between rats of the TNBS-colitis and the vehicle-treated groups, whereas treatment with CAPE 10 and 30 mg/kg, respectively, caused a significant reduction in colon injury compared to that observed in rats of the TNBS-colitis and vehicle-treated groups. The histologies of both treatment groups were not significantly different. In terms of the biochemical analyses, myeloperoxidase levels in rats from the CAPE 10 and 30 mg/kg groups were significantly different from that of the colitis control rats; however, the levels of malondialdehyde (MDA), catalase and reduced glutathione (GSH) were only significantly different from the levels found colitis control rats in rats administered 10 mg/kg. The levels of MDA, GSH and SOD in rats given CAPE were also significantly different from those of rats in the vehicle control group. These results were consistent with histological findings. Conclusion CAPE may have a positive effect on the inflammatory bowel disease treatment process and could, therefore, be used as an adjunct therapy in colitis. These effects of CAPE may occur through anti-inflammatory and antioxidant mechanisms.

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Title: The Effects of Caffeic Acid Phenethyl Ester (CAPE) on TNBS-induced Colitis in Ovariectomized Rats
Authors: Rauf Onur Ek
Mukadder Serter
Kemal Ergin
Yuksel Yildiz
Serpil Cecen
Tulay Kavak
Cigdem Yenisey
Publication date: 01-06-2008
Publisher: Springer US
Published in: Digestive Diseases and Sciences / Issue 6/2008
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-007-0056-2

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