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Open Access 01-12-2014 | Preliminary research

The effect of volume of interest definition on quantification of lymph node immune response to a monkeypox virus infection assessed by ¹⁸F-FDG-PET

Authors: Svetlana Chefer, Richard C Reba, Christopher Z Leyson, Jurgen Seidel, Reed F Johnson, Joseph E Blaney, Peter B Jahrling, Julie Dyall

Published in: EJNMMI Research | Issue 1/2014

Abstract

Background

2-deoxy-2-[¹⁸F]fluoro-D-glucose-positron emission tomography (¹⁸F-FDG-PET) is applied in the clinic for infection assessment and is under consideration for investigating the inflammatory/immune response in lymphoid tissue in animal models of viral infection. Assessing changes in ¹⁸F-FDG uptake of lymph nodes (LNs), primary lymphoid tissues targeted during viral infection, requires suitable methods for image analysis. Similar to tumor evaluation, reliable quantitation of the LN function via multiple ¹⁸F-FDG-PET sessions will depend how the volume of interest is defined. Volume of interest definition has a direct effect on statistical outcome. The current study objective is to compare for the first time agreement between conventional and modified VOI metrics to determine which method(s) provide(s) reproducible standardized uptake values (SUVs) for ¹⁸F-FDG uptake in the LN of rhesus macaques.

Methods

Multiple ¹⁸F-FDG-PET images of LNs in macaques were acquired prior to and after monkeypox virus intravenous inoculation. We compared five image analysis approaches, SUV_max, SUV_mean, SUV_threshold, modified SUV_threshold, and SUV_{fixed volume}, to investigate the impact of these approaches on quantification of the changes in LN metabolic activity denoting the immune response during viral infection progression.

Results

The lowest data repeatability was observed with SUV_max. The best correspondence was between SUV_{fixed volume} and conventional and modified SUV_threshold. A statistically significant difference in the LN ¹⁸F-FDG uptake between surviving and moribund animals was shown using modified SUV_threshold and SUV_{fixed volume} (adjusted p = 0.0037 and p = 0.0001, respectively).

Conclusions

Quantification of the LN ¹⁸F-FDG uptake is highly sensitive to the method applied for PET image analysis. SUV_{fixed volume} and modified SUV_threshold demonstrate better reproducibility for SUV estimates than SUV_max, SUV_mean, and SUV_threshold. SUV_{fixed volume} and modified SUV_threshold are capable of distinguishing between groups with different disease outcomes. Therefore, these methods are the preferred approaches for evaluating the LN function during viral infection by ¹⁸F-FDG-PET. Validation of multiple approaches is necessary to choose a suitable method to monitor changes in LN metabolic activity during progression of viral infection.

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Title: The effect of volume of interest definition on quantification of lymph node immune response to a monkeypox virus infection assessed by 18F-FDG-PET
Authors: Svetlana Chefer
Richard C Reba
Christopher Z Leyson
Jurgen Seidel
Reed F Johnson
Joseph E Blaney
Peter B Jahrling
Julie Dyall
Publication date: 01-12-2014
Publisher: Springer Berlin Heidelberg
Published in: EJNMMI Research / Issue 1/2014
Electronic ISSN: 2191-219X
DOI: https://doi.org/10.1186/s13550-014-0049-z

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Springer Medicine

The effect of volume of interest definition on quantification of lymph node immune response to a monkeypox virus infection assessed by ¹⁸F-FDG-PET

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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