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Clinical Oral Investigations

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01-01-2018 | Original Article

The effect of triclosan on posttranslational modification of proteins through citrullination and carbamylation

Authors: Richard Bright, C. Marchant, P. M. Bartold

Published in: Clinical Oral Investigations | Issue 1/2018

Abstract

Objectives

The aim of this study was evaluate the effect of triclosan on citrullination and carbamylation, two important protein posttranslational modifications associated with inflammatory conditions such as periodontitis and rheumatoid arthritis.

Materials and methods

A range of triclosan concentrations were incubated in the presence of appropriate substrates used for the generation of either citrullinated or carbamylated proteins. The effect of triclosan on protein citrullination and carbamylation in macrophages was also assessed.

Results

Citrullination and carbamylation were both significantly decreased by triclosan at concentrations six times lower than the 0.3% triclosan approved by the FDA to use in mouthwash and toothpaste. When macrophages were exposed to triclosan, carbamylation was significantly deceased (p = 0.01), and while citrullination also decreased, this reduction was not statistically significant (p = 0.06).

Conclusion

Triclosan reduced the generation of protein citrullination and carbamylation in vitro.

Clinical relevance

Triclosan may be useful as an adjunct therapy in the management of inflammatory periodontal diseases and help to reduce posttranslational protein modification citrullination and carbamylation) in these tissues.

Eke PI et al (2012) Prevalence of periodontitis in adults in the United States: 2009 and 2010. J Dent res 91(10):914–920CrossRefPubMed

Demmer RT, Papapanou PN (2010) Epidemiologic patterns of chronic and aggressive periodontitis. Periodontol 2000 53:28–44CrossRefPubMedPubMedCentral

Arigbede AO, Babatope BO, Bamidele MK (2012) Periodontitis and systemic diseases: a literature review. J Indian Soc Periodontol 16(4):487–491CrossRefPubMedPubMedCentral

Linden GJ, Lyons A, Scannapieco FA (2013) Periodontal systemic associations: review of the evidence. J Clin Periodontol 40(Suppl 14):S8–19PubMed

Kaur S et al (2014) Does periodontal treatment influence clinical and biochemical measures for rheumatoid arthritis? A systematic review and meta-analysis. Semin Arthritis Rheum 44(2):113–122CrossRefPubMed

Akram Z, Safii SH, Vaithilingam RD et al (2016) Efficacy of non-surgical periodontal therapy in the management of chronic periodontitis among obese and non-obese patients: a systematic review and meta-analysis. Clin Oral Investig 20(5):903–914

Mercanoglu F et al (2004) Endothelial dysfunction in patients with chronic periodontitis and its improvement after initial periodontal therapy. J Periodontol 75(12):1694–1700CrossRefPubMed

Perez-Losada FL, Jané-Salas E, Sabater-Recolons MM et al (2016) Correlation between periodontal disease management and metabolic control of type 2 diabetes mellitus. A systematic literature review. Med Oral Patol Oral Cir Bucal 21(4):e440–e446

Sreenivasan PK, Gaffar A (2008) Antibacterials as anti-inflammatory agents: dual action agents for oral health. Anton Leeuw Int J gen Mol Microbiol 93(3):227–239CrossRef

10.

Gaffar A et al (1995) The effect of triclosan on mediators of gingival inflammation. J Clin Periodontol 22(6):480–484CrossRefPubMed

11.

Barros SP et al (2010) Triclosan inhibition of acute and chronic inflammatory gene pathways. J Clin Periodontol 37(5):412–418CrossRefPubMed

12.

Modeer T, Bengtsson A, Rolla G (1996) Triclosan reduces prostaglandin biosynthesis in human gingival fibroblasts challenged with interleukin-1 in vitro. J Clin Periodontol 23(10):927–933CrossRefPubMed

13.

Fine DH et al (2006) The antimicrobial effect of a triclosan/copolymer dentifrice on oral microorganisms in vivo. J am Dent Assoc 137(10):1406–1413CrossRefPubMed

14.

Vazquez J et al (2005) Clinical efficacy of a triclosan/copolymer/NaF dentifrice and a commercially available breath-freshening dentifrice on hydrogen sulfide-forming bacteria. Oral dis 11(Suppl 1):64–66CrossRefPubMed

15.

Hu D et al (2008) A clinical investigation of the efficacy of two dentifrices for controlling oral malodor and plaque microflora overnight. J Clin Dent 19(3):106–110PubMed

16.

Davies RM, Ellwood RP, Davies GM (2004) The effectiveness of a toothpaste containing triclosan and polyvinyl-methyl ether maleic acid copolymer in improving plaque control and gingival health: a systematic review. J Clin Periodontol 31(12):1029–1033CrossRefPubMed

17.

Riley P, Lamont T (2013) Triclosan/copolymer containing toothpastes for oral health. Cochrane Database Syst Rev, 12:Cd010514

18.

Blinkhorn A et al (2009) Is there a role for triclosan/copolymer toothpaste in the management of periodontal disease? Br Dent J 207(3):117–125CrossRefPubMed

19.

Bartold PM, Marshall RI, Haynes DR (2005) Periodontitis and rheumatoid arthritis: a review. J Periodontol 76(11 Suppl):2066–2074CrossRefPubMed

20.

El-Gabalawy H (2009) The preclinical stages of RA: lessons from human studies and animal models. Best Pract Res Clin Rheumatol 23(1):49–58CrossRefPubMed

21.

Shi J et al (2011) Autoantibodies recognizing carbamylated proteins are present in sera of patients with rheumatoid arthritis and predict joint damage. Proc Natl Acad Sci U S A 108(42):17372–17377CrossRefPubMedPubMedCentral

22.

Zeltz C, Gullberg D (2014) Post-translational modifications of integrin ligands as pathogenic mechanisms in disease. Matrix Biol 40:5–9CrossRefPubMed

23.

Vossenaar ER et al (2003) PAD, a growing family of citrullinating enzymes: genes, features and involvement in disease. BioEssays 25(11):1106–1118CrossRefPubMed

24.

Wegner N et al (2010) Peptidylarginine deiminase from Porphyromonas gingivalis citrullinates human fibrinogen and alpha-enolase: implications for autoimmunity in rheumatoid arthritis. Arthritis Rheum 62(9):2662–2672CrossRefPubMedPubMedCentral

25.

Jang B et al (2012) Peptidylarginine deiminase modulates the physiological roles of enolase via citrullination: links between altered multifunction of enolase and neurodegenerative diseases. Biochem J 445(2):183–192CrossRefPubMed

26.

Valesini G et al (2015) Citrullination and autoimmunity. Autoimmun rev 14(6):490–497CrossRefPubMed

27.

Mohanan S et al (2012) Potential role of peptidylarginine deiminase enzymes and protein citrullination in cancer pathogenesis. Biochem res Int 2012:895343CrossRefPubMedPubMedCentral

28.

Sirpal S (2009) Myeloperoxidase-mediated lipoprotein carbamylation as a mechanistic pathway for atherosclerotic vascular disease. Clin Sci (Lond) 116(9):681–695CrossRef

29.

Pietrement C et al (2013) Chronic increase of urea leads to carbamylated proteins accumulation in tissues in a mouse model of CKD. PLoS One 8(12):e82506CrossRefPubMedPubMedCentral

30.

Wang Z et al (2007) Protein carbamylation links inflammation, smoking, uremia and atherogenesis. Nat Med 13(10):1176–1184CrossRefPubMed

31.

Rosenstein ED et al (2004) Hypothesis: the humoral immune response to oral bacteria provides a stimulus for the development of rheumatoid arthritis. Inflammation 28(6):311–318CrossRefPubMed

32.

Bright R et al (2015) Is there a link between carbamylation and citrullination in periodontal disease and rheumatoid arthritis? Med Hypotheses 84(6):570–576

33.

Knipp M, Vasak M (2000) A colorimetric 96-well microtiter plate assay for the determination of enzymatically formed citrulline. Anal Biochem 286(2):257–264CrossRefPubMed

34.

Rosen G et al (1995) Proteases of treponema-denticola outer sheath and extracellular vesicles. Infect Immun 63(10):3973–3979PubMedPubMedCentral

35.

Sivashanmugam A et al (2009) Practical protocols for production of very high yields of recombinant proteins using Escherichia coli. Protein Sci 18(5):936–948CrossRefPubMedPubMedCentral

36.

Ok E et al (2005) Carbamylated low-density lipoprotein induces death of endothelial cells: a link to atherosclerosis in patients with kidney disease. Kidney Int 68(1):173–178CrossRefPubMed

37.

Marchant C et al (2013) Effect of Porphyromonas gingivalis on citrullination of proteins by macrophages in vitro. J Periodontol 84(9):1272–1280CrossRefPubMed

38.

Zhou Y et al (2015) Characterization of the hypercitrullination reaction in human neutrophils and other leukocytes. Mediat Inflamm 2015:236451CrossRef

39.

Holzer M et al (2011) Protein carbamylation renders high-density lipoprotein dysfunctional. Antioxid Redox Signal 14(12):2337–2346CrossRefPubMedPubMedCentral

40.

Bratthauer GL (2010) The avidin-biotin complex (ABC) method and other avidin-biotin binding methods. Methods Mol Biol 588:257–270CrossRefPubMed

41.

Taylor CR, Levenson RM (2006) Quantification of immunohistochemistry—issues concerning methods, utility and semiquantitative assessment II. Histopathology 49(4):411–424CrossRefPubMed

Title: The effect of triclosan on posttranslational modification of proteins through citrullination and carbamylation
Authors: Richard Bright
C. Marchant
P. M. Bartold
Publication date: 01-01-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 1/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2137-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The effect of triclosan on posttranslational modification of proteins through citrullination and carbamylation

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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